Temperature-dependent stimulation and inhibition of adenylate cyclase by Aplysia bag cell peptides

Add time:08/01/2019    Source:sciencedirect.com

The bag cell peptides (α-, β-, and γ-BCP) are secreted by the neuroendocrine bag cells of Aplysia, and provide feedback modulation of bag cell excitability and cAMP levels. We report here that if 200–500 mM NaCL is included in the assay buffer, the BCPs alter adenylate cyclase activity in a manner consistent with their effects on cAMP levels in intact bag cells. Specifically, β-BCP and the related peptide A from the atrial gland stimulate the enzyme, while the effects of α-BCP(1–7) and γ-BCP are temperature dependent, stimulating at 30°C and inhibiting at 15°C. Both stimulation and inhibition require GTP, suggesting mediation by Gs and Gi. The ionic requirements of stimulation and inhibition differ: C− is necessary to support stimulation, but not inhibition. Moreover, pertussis toxin blocks inhibition, but does not affect stimulation. These results suggest that the temperature-sensitive mechanism lies upstream from the G-protein in the signal transduction pathway.

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