Neuropeptide processing activity in Aplysia bag cell homogenates

Add time:08/06/2019    Source:sciencedirect.com

The neurosecretory bag cells of the mollusk, Aplysia, generate a peptide egg-laying hormone (ELH) from a 29,000 Dalton precursor protein by proteolytic cleavage to a 6–9,000 Dalton intermediate, followed by cleavage of the intermediate. We report here the initial characterization of these cleavage activities. Homogenates of bag cells in low ionic strength buffer process endogenous precursor to a peptide which is indistinguishable from ELH in molecular weight and isoelectric point. Non-specific proteolysis in the homogenates is not detectable. The pH optimum for cleavage of the precursor and the intermediate is 5.5–6.5. The cleavage activities exhibit a substantial degree of membrane association, and the inhibitor profile of each is characteristic of a thiol protease without a metal cofactor requirement. Precursor cleavage activity differs from that of the intermediate cleaving activity in inhibitor profile, solubility, and, slightly, in pH optimum.

We also recommend Trading Suppliers and Manufacturers of bag cell peptide (Aplysia) (2-8) (cas 104186-26-7). Pls Click Website Link as below: cas 104186-26-7 suppliers

Prev:A calfluxin-related peptide is present in the bag cells and atrial gland of Aplysia
Next:The bag cell egg-laying hormones of Aplysia brasiliana and Aplysia californica are identical)