A unique glycosphingolipid-splitting enzyme (ceramide-glycanase from leech) cleaves the linkage between the oligosaccharide and the ceramide

Add time:08/08/2019    Source:sciencedirect.com

SummaryA novel type of enzyme which hydrolyzes the linkage between the ceramide and the sugar chain in various glycosphingolipids has been found in the leech, Hirudomedicinalis. This enzyme releases the intact oligosaccharide from LacCer, GbOse3Cer, GbOse4Cer, GbOse5Cer, nLcOse4Cer, GM3, GM2, GM1, GD1a and GT1 with the concurrent release of ceramides. By using tritium-labeled GM1 as substrate we found the optimum pH of this enzyme to be between pH 4 and 5. Since the enzyme cleaves the linkage between the ceramide and the sugar chain in various glycosphingolipids with no apparent preference toward the sugar chain, we propose to call this enzyme ceramide-glycanase.

We also recommend Trading Suppliers and Manufacturers of CERAMIDE GLYCANASE (cas 106769-66-8). Pls Click Website Link as below: cas 106769-66-8 suppliers

Prev:Synthesis of bi-fluorescence-labeled lactoside: A substrate for continual assay of CERAMIDE GLYCANASE (cas 106769-66-8)
Next:High-Performance Liquid Chromatography Analysis of Ganglioside Carbohydrates at the Picomole Level after CERAMIDE GLYCANASE (cas 106769-66-8) Digestion and Fluorescent Labeling with 2-Aminobenzamide)