Experimental ApproachesPulmonary Microvascular and Alveolar Epithelial Permeability Characteristics in N-Nitroso-N-METHYLURETHANE (cas 105-40-8)-Injected Dogs

Add time:08/16/2019    Source:sciencedirect.com

The subcutaneous injection of 5 to 6 mg/kg of body weight of N-nitroso-N-methylurethane (NNNMU) has been reported to cause acute alveolar injury in animals. To determine the permeability characteristics of the alveolar epithelium, we employed the in vivo saline-filled dog lung model and determined the time to 50 percent equilibration in minutes of a specific tracer in the blood and the lung liquid (T½) for endogenous serum albumin (MW 69,000 daltons, molecular radius 35 Å) and exogenously administered 500,000 MW polydispersed dextrans (@ molecular radius 200 A). Compared to control animals, T½ decreased (permeability increased) in NNNMU-injected dogs from 3,500±100 to 682±160 minutes for albumin and from 20,000±250 to 2,790±750 minutes for 500,000 MW dextran (P < 0.001). To determine the permeability characteristics of the pulmonary microvasculature, we employed the right lymph duct cannulation dog model and measured lymph flow/30 minutes, lymph albumin and dextran concentration, and lymph/plasma albumin and dextran ratios in control and NNNMU-injected dogs. Compared to control animals, lymph flow was significantly greater in NNNMU dogs, 2.07±1.1 vs .71±.50 ml/30 minutes (P < 0.01), respectively. We conclude that NNNMU injection increases permeability in both the alveolar epithelium and the pulmonary microvasculature.

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