Calcium uptake in mitochondria and vesicles of heart and skeletal muscle in presence of potassium, sodium, K-STROPHANTHIN (cas 11005-63-3) and pentobarbital☆

Add time:08/23/2019    Source:sciencedirect.com

45Ca++ uptake in mitochondria and vesicles of heart and skeletal muscle was measured in the presence of a low concentration of calcium. The following results were obtained. (1) In mitochondria there was a rapid and brief Ca++ uptake if succinate was present; when ATP was added, Ca++ uptake was slow and prolonged; cyanide prevented the rapid Ca++ accumulation and azide inhibited the slow Ca++ uptake. In vesicles ATP was required for Ca++ uptake and this was increased by oxalate, phosphate, or an ATP regenerating system. (2) K-STROPHANTHIN (cas 11005-63-3) (10−8–10−4M) had no significant effect on Ca++ uptake in mitochondria or vesicles. (3) Pentobarbital (1–6 mM) inhibited Ca++ uptake in mitochondria of heart and white and red skeletal muscle but had no effect on Ca++ uptake in vesicles. (4) The K+:Na+ ratio in the incubation medium had an effect on Ca++ uptake in mitochondria and vesicles of heart muscle and in mitochondria of red skeletal muscle. In mitochondria Ca++ uptake was minimal at the K+:Na+ ratio of 1, and raising the K+: Na+ ratio up to 10 approximately doubled Ca++ uptake. In vesicles the substitution of Na+ for K+ in the incubation medium diminished Ca++ uptake, if Ca++ uptake was not increased by oxalate, phosphate, or an ATP regenerating system.

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