Enzymatic synthesis of β-[U-14C]alanine and d-[1,2,3-14C]pantothenate of high specific radioactivity
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Add time:08/16/2019 Source:sciencedirect.com
β-[U-14C]Alanine can be synthesized in >95% yield from l-[U-14C]aspartic acid using the aspartate 1-decarboxylase of Escherichia coli and converted to d-[1,2,3-14C]pantothenate in a 10–20% yield using the pantothenate synthetase of E. coli. Sufficiently pure preparations of both enzymes are readily obtained.
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