Separation of 5′-dimethoxytrityl-2′-deoxynucleoside-3′-omethylphosphonamidite diastereomers by normal phase chromatography

Add time:08/16/2019    Source:sciencedirect.com

Rp and Sp diastereomers of the 5′-dimethoxytrityl-2′-deoxynucleoside-3′-Omethylphosphonamidites of N6-Benzoyl-adenine, N2-isobutyryl-guanine, N4-benzoylcytosine, and thymine were separated by liquid chromatography on a normal phase silica column eluted with 1.0% EtOH in CHCl3.

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