Isolation of mutant adenosine deaminase by coformycin (cas 11033-22-0) affinity chromatography☆

Add time:08/18/2019    Source:sciencedirect.com

Adenosine deaminase is a purine salvage enzyme that catalyzes the deamination of adenosine and deoxyadenosine. Deficiency of the enzyme activity is associated with T-cell and B-cell dysfunction. Mutant adenosine deaminase has been isolated from heterozygous and homozygous deficient lymphoblast cell lines with the aid of an affinity matrix consisting of coformycin (cas 11033-22-0) (a potent inhibitor of the enzyme) as the affinity ligand, bound to 3,3′-iminobispropylamine-derivatized Sepharose. Routinely, 80–90% of adenosine deaminase in crude cell homogenates could be bound to the material. Adenosine deaminase was specifically eluted by enzyme inhibitors or less efficiently by high substrate concentrations. Protein preparations isolated from several different deficient cell lines were highly purified and exhibited molecular weights identical to wild-type adenosine deaminase. This method produces a protein that is suitable for structural studies.

We also recommend Trading Suppliers and Manufacturers of coformycin (cas 11033-22-0). Pls Click Website Link as below: cas 11033-22-0 suppliers

Prev:A novel metabolic effect of the adenosine deaminase inhibitor coformycin (cas 11033-22-0), a potentiator of antiviral adenosine analogues
Next:S-adenosylhomocysteine hydrolase activity, deoxyadenosine triphosphate accumulation, and competence of thymocyte and spleen leucocyte response to mitogens in coformycin (cas 11033-22-0)-treated mice☆)