Generation and characterization of a monoclonal antibody specific for human herpesvirus 6 variant A immediate–early 2 protein

Add time:08/26/2019    Source:sciencedirect.com

Two variants (A and B) of human herpesvirus 6 (HHV-6) can be isolated from humans, with each variant having unique biological properties. HHV-6 variant typing is mainly done following amplification of viral genomic DNA followed by restriction endonuclease digestion. Our objective was to generate a monoclonal antibody (mAb) that would allow us to discriminate between variants A and B of HHV-6. BALB/c mice were immunized with a recombinant glutathione-S-transferase protein fused to the immediate–early (IE) 2 protein from HHV-6 variant A. Following splenocytes fusion, one IgG1 κ light chain mAb (P6H8) was isolated and found to react specifically with variant A IE2 protein in immunofluorescence and western blot assays. The P6H8 antibody represents a useful tool for both fundamental research and clinical applications allowing for the discrimination of infections caused by HHV-6 variants A or B.

We also recommend Trading Suppliers and Manufacturers of human herpesvirus 6 p41 protein (cas 138415-18-6). Pls Click Website Link as below: cas 138415-18-6 suppliers

Prev:Research paperGrowth of human herpesvirus 6 in HEPG2 cells
Next:Sensitivity of human herpesvirus 6 and other human herpesviruses to the broad-spectrum antiinfective drug artesunate)