Synthesis and hybridizing properties of isoDNAs including 3′-O,4′-C-ethyleneoxy-bridged 5-methyluridine derivatives

Add time:08/28/2019    Source:sciencedirect.com

3′,4′-Ethyleneoxy-bridged 5-methyluridine derivatives with methyl groups in the bridge, (R)-Me-3′,4′-EoNA-T and (S)-Me-3′,4′-EoNA-T, were synthesized, and these two analogs and unsubstituted 3′,4′-EoNA-T were successfully incorporated into a 2′,5′-linked oligonucleotide (isoDNA). Their duplex-forming ability with complementary DNA and complementary RNA, and triplex-forming ability with double-stranded DNA, were evaluated by UV-melting experiments. The results indicated that isoDNAs, including these 3′,4′-EoNA analogs, could hybridize exclusively with complementary RNA. In particular, 3′,4′-EoNA-T and (R)-Me-3′,4′-EoNA-T modifications within isoDNA could stabilize the duplexes with complementary RNA compared with unmodified or 3′,4′-BNA-modified isoDNAs.

We also recommend Trading Suppliers and Manufacturers of 5'-O-(DIMETHOXYTRITYL)-2'-DEOXYNEBULARINE (cas 108310-90-3). Pls Click Website Link as below: cas 108310-90-3 suppliers

Prev:4,4′-Dimethoxytrityl group derived from secondary alcohols: Are they removed slowly under acidic conditions?
Next:Activation of human RNase L by 2′- and 5′-O-methylphosphonate-modified oligoadenylates)