Molecular characterization of a short NEUROPEPTIDE F (cas 136697-70-6) signaling system in the tsetse fly, Glossina morsitans morsitans
-
Add time:09/09/2019 Source:sciencedirect.com
Neuropeptides of the short NEUROPEPTIDE F (cas 136697-70-6) (sNPF) family are widespread among arthropods and found in every sequenced insect genome so far. Functional studies have mainly focused on the regulatory role of sNPF in feeding behavior, although this neuropeptide family has pleiotropic effects including in the control of locomotion, osmotic homeostasis, sleep, learning and memory. Here, we set out to characterize and determine possible roles of sNPF signaling in the haematophagous tsetse fly Glossina morsitans morsitans, a vector of African Trypanosoma parasites causing human and animal African trypanosomiasis. We cloned the G. m. morsitans cDNA sequences of an sNPF-like receptor (Glomo-sNPFR) and precursor protein encoding four Glomo-sNPF neuropeptides. All four Glomo-sNPF peptides concentration-dependently activated Glomo-sNPFR in a cell-based calcium mobilization assay, with EC50 values in the nanomolar range. Gene expression profiles in adult female tsetse flies indicate that the Glomo-sNPF system is mainly restricted to the nervous system. Glomo-snpfr transcripts were also detected in the hindgut of adult females. In contrast to the Drosophila sNPF system, tsetse larvae lack expression of Glomo-snpf and Glomo-snpfr genes. While Glomo-snpf transcript levels are upregulated in pupae, the onset of Glomo-snpfr expression is delayed to adulthood. Expression profiles in adult tissues are similar to those in other insects suggesting that the tsetse sNPF system may have similar functions such as a regulatory role in feeding behavior, together with a possible involvement of sNPFR signaling in osmotic homeostasis. Our molecular data will enable further investigations into the functions of sNPF signaling in tsetse flies.
We also recommend Trading Suppliers and Manufacturers of NEUROPEPTIDE F (cas 136697-70-6). Pls Click Website Link as below: cas 136697-70-6 suppliers
Prev:ReportDrosophila NEUROPEPTIDE F (cas 136697-70-6) Signaling Independently Regulates Feeding and Sleep-Wake Behavior
Next:Regulation of sleep by the short NEUROPEPTIDE F (cas 136697-70-6) (sNPF) in Drosophila melanogaster) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- NEUROPEPTIDE F (cas 136697-70-6) peptides act through unique signaling pathways to affect cardiac activity09/24/2019
- Regulation of sleep by the short NEUROPEPTIDE F (cas 136697-70-6) (sNPF) in Drosophila melanogaster09/10/2019
- ReportDrosophila NEUROPEPTIDE F (cas 136697-70-6) Signaling Independently Regulates Feeding and Sleep-Wake Behavior09/08/2019
- The short NEUROPEPTIDE F (cas 136697-70-6) modulates olfactory sensitivity of Bactrocera dorsalis upon starvation09/07/2019
- Characterization of a NEUROPEPTIDE F (cas 136697-70-6) receptor in the tsetse fly, Glossina morsitans morsitans09/06/2019
- Chapter 41 - NEUROPEPTIDE F (cas 136697-70-6)09/05/2019
- Functional characterization of mosquito short NEUROPEPTIDE F (cas 136697-70-6) receptors09/04/2019
- Optogenetic activation of short NEUROPEPTIDE F (cas 136697-70-6) (sNPF) neurons induces sleep in Drosophila melanogaster09/03/2019
-
Health and Chemical more >