Oxidative stress induced by oxime reactivators of acetylcholinesterase in vitro

Add time:09/26/2019    Source:sciencedirect.com

In this study, we determined the effect of methoxime (MMB-4), asoxime (HI-6), obidoxime (LüH-6), trimedoxime (TMB-4), and pralidoxime (2-PAM) on redox homeostasis in vitro. Cultured human hepatoma cells (HepG2) were exposed to oximes at concentrations equivalent to their IC50 (assessed using MTT assay) and evaluated 1, 4 and 24 h after incubation. Additionally, intact, early and late apoptotic and necrotic cells were quantified by microcapillary flow cytometry. Intracellular levels of oxygen/nitrogen species were determined using two fluorescent probes (2′,7′-dichlorodihydrofluorescein diacetate and dihydroethidium). Malondialdehyde and 3-nitrotyrosine were measured by LC-MS/MS. Non-protein thiols and non-protein disulfides were evaluated using HPLC-UV to reflect antioxidant capacity. Oxidative and nitrosative stress was induced by LüH-6, TMB-4 and MMB-4, whereas 2-PAM and HI-6 appeared as weak oxidative stressors with no activity towards nitrosative stress in HepG2 cells. Based on these results, bisquartenary oxime reactivators containing two functional oxime groups at the position 4 of pyridinium ring appear as more intense oxidative and nitrosative inducers. Activation of apoptosis and necrosis do not seem to correlate with generation of RONS. On the other hand, both processes rather reflect MDA concentrations, i.e. the damage of biomolecules.

We also recommend Trading Suppliers and Manufacturers of Acetone oxime (cas 127-06-0). Pls Click Website Link as below: cas 127-06-0 suppliers

Prev:Original articleSynthesis and anti-inflammatory activity of novel (substituted)benzylidene Acetone oxime (cas 127-06-0) ether derivatives: Molecular modeling study
Next:Controlled synthesis of poly(Acetone oxime (cas 127-06-0) acrylate) as a new reactive polymer: Stimuli-responsive reactive copolymers)