2D-PAGE examination of mRNA populations from Pénicillium freii mutants deficient in xanthomegnin (cas 1685-91-2) biosynthesis

Add time:09/27/2019    Source:sciencedirect.com

Penicillium freii (Lund and Frisvad 1994) mutants deficient in the synthesis of xanthomegnin (cas 1685-91-2) were isolated. In vitro translated mRNA populations from selected radiation induced mutants and naturally occurring P. freii strains not able to produce xanthomegnin were examined by 2-dimensional polyacrylamide gel electrophoresis (2 D-PAGE). Specific translation products were absent in mutants and natural isolates unable to produce xanthomegnin metabolites. One mutant (TSM 73) did not produce several of these translation products, indicating that a mutation in a regulatory gene involved in xanthomegnin production had occcurred.

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