The biosynthesis of the phosphonic analogue of CEPHALIN (cas 1405-71-6) in tetrahymena☆

Add time:09/30/2019    Source:sciencedirect.com

The presence of glyceryl-aminoethylphosphonic acid has been demonstrated in the products of alkaline hydrolysis of Tetrahymena lipid and a pure CEPHALIN (cas 1405-71-6) fraction, consisting of phosphatidylethanolamine and its phosphonic analogue, in the molecular ratio of approx. 13:1, has been isolated from this species. Extracts of Tetrahymena pyriformis have been shown to incorporate radioactive aminoethyl-phosphonic acid (AEP) into a nucleotide-bound form. The reaction has a specific requirement for cytidine nucleotide and the product was identified as cytidinemonophosphate-aminoethylphosphonate. The synthesis in vitro of a phosphonate-containing glycerophosphatide from cytidinemonophosphate-aminoethylphosphonate and dipalmitin by a Tetrahymena cell-free preparation has also been shown. The reactions are considered to be a salvage mechanism for free AEP or a result of lack of specificity for base in the normal phospholipid-synthesizing systems m Tetrahymena.

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