Reference

Determination of catechols

Determination of catechols. (Honda, Susumu; Kanto Chemical Co., Inc., Japan). Jpn. Kokai Tokkyo Koho JP 59042450 A2 9 Mar 1984 Showa, 7 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: G01N031-22; G01N021-76. APPLICATION: JP 82-151807 2 Sep 1982. DOCUMENT TYPE: Patent CA Section: 2 (Mammalian Hormones) Section cross-reference(s): 9 A sample contg. catechols (epinephrine [51-43-4], norepinephrine [107-91-5], dopamine [51-61-6], isoproterenol [7683-59-2], L-dopa [59-92-7], 3,4-dihydoxyphenylacetic acid [102-32-9], 3,4-dihydroxymandelic acid [775-01-9], 3,4-dihydroxybenzoic acid [99-50-3], pyrocatechol [120-80-9]) is treated with 2-cyanoacetamide [107-91-5] for fluorometric detn. 51-43-4 and 120-80-9 are also in the experiment. Unlike conventional methods, this method is highly sensitive. The method can be used in the anal. of urine and blood serum samples. Absorbance was linearly related to concns. of dopamine, norepinephrine, and epinephrine (up to 10-4M). .

N-Acetylcyanamide, the major urinary metabolite of cyanamide in rat, rabbit, dog, and man

N-Acetylcyanamide, the major urinary metabolite of cyanamide in rat, rabbit, dog, and man. Shirota, F. N.; Nagasawa, H. T.; Kwon, C. H.; Demaster, E. G. (Med. Res. Lab., Veterans Adm. Med. Cent., Minneapolis, MN 55417, USA). Drug Metab. Dispos., 12(3), 337-44 (English) 1984. CODEN: DMDSAI. ISSN: 0090-9556. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The structure of the major urinary metabolite of cyanamide [420-04-2], the active component of the alc. deterrent agents Temposil, Dipsan, and Abstem, in rats, rabbits, and dogs was established to be N-acetylcyanamide [107-91-5] by chem. synthesized, and conversion of the metabolite and synthetic product to identical derivs., viz. to N-benzyl- and N-(p-nitrobenzyl)-N-acetylcyanamide. The latter derivs. were analyzed by pulsed pos./neg. ion chem. ionization mass spectroscopy. Urine from patients receiving cyanamide for treatment was shown to contain N-acetylcyanamide by chem. ionization mass spectrometric anal. of the isolated p-nitrobenzyl deriv., thereby establishing that N-acetylcyanamide is also a metabolite in man. The major portion (87%) of the 1st 27 h urinary radioactivity excreted by the dog after receiving a los dose of [14C]cyanamide (0.04 mmol/kg, orally) was N-acetylcyanamide, as detd. by inverse isotope diln. and measurement of the specific radioactivity of its N-p-nitrobenzyl deriv. This indicates that at low doses, acetylation is also a major route of biotransformation of cyanamide in the dog. Hepatic N-acetyltransferase [9012-30-0], isolated from the rabbit and dog, catalyzed the transfer of the acetyl group from acetyl-S-CoA to [14C]cyanamide producing N-acetyl[14C]cyanamide. The enzyme isolated from the liver of a rapid acetylator phenotype rabbit was twice as effective as the dog enzyme in catalyzing this transfer. Thus, the enzyme responsible for this biotransformation of cyanamide is an acetyl-S-CoA-dependent N-acetyltransferase.