54827-17-7Relevant articles and documents
Enzyme mimicking inorganic hybrid Ni@MnO2 for colorimetric detection of uric acid in serum samples
Pal, Jaya,Pal, Tarasankar
, p. 83738 - 83747 (2016)
Reliable and inexpensive detection of uric acid in the absence of any peroxide or enzyme is very challenging for the development of a new cost effective clinical method. Herein, we report an easy, cost effective and nonenzymetic method for selective detection and quantification of uric acid using 3,3′,5,5′-tetramethylbenzidine (TMB) and a Ni@MnO2 hybrid nanomaterial. The ultra-long porous core-shell Ni@MnO2 hybrid nanomaterial, obtained from a simple redox transformation reaction between a prickly nickel nanowire and KMnO4 solution, is reported for the first time. The growth mechanism is studied from time-dependent FESEM and TEM images. The hybrid nanomaterial exhibited intrinsic oxidase-like activity that helps catalytic oxidation of TMB (colourless) to a blue colored product which becomes colourless in the presence of uric acid. Moreover, this colorimetric method has been exploited for the assay of serum uric acid in real samples. Our proposed method can detect as low as 0.24 μM of uric acid with a linear range from 1 to 40 μM. Therefore, we believe that this method would be useful for the detection of uric acid in clinical laboratory.
Versatile, concise and convenient process for the commercial scale preparation of highest pure 3,3'5,5'-Tetramethylbenzidine (TMB) and its salts, a chromogenic substrate used in staining procedures in immunohistochemistry and visualizing reagent in enzyme-linked immunosorbent assays
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Paragraph 0031-0032, (2021/04/23)
The present invention relates to a process for the preparation of 3,3′,5,5′-Tetramethylbenzidine represented by formula I, and processes for the preparation of intermediates used in the preparation of 3,3′,5,5′-Tetramethylbenzidine.
Synthesis method of biochemical preparation TMB
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, (2018/03/26)
The invention discloses a synthesis method of a biochemical preparation TMB. The synthesis method includes following steps: synthesizing 1,2, 2', 6, 6'-azo tetratoluidine-N, N-dioxide; synthesizing 1,2, 2', 6, 6'-tetramethyl diphenylhydrazine; synthesizing 3,TMB (1/2)H2SO4; preparing 4,TMB 2HCl; preparing TMB. The synthesis method is simple in synthesis route, cheap and easy-to-obtain in raw material, low in synthesis cost and high in yield.
A 3, 3 ', 5, 5' - tetramethyl benzidine and its hydrochloride synthesis method (by machine translation)
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Paragraph 0020-0022; 0024; 0025, (2019/01/08)
The present invention provides a synthetic 3, 3 ', 5, 5' - tetramethyl benzidine and its hydrochloride method, which belongs to the technical field of organic synthesis. In order to 2, 6 - dimethyl aniline as the raw material, first of all through the potassium permanganate oxidation of 2, 2 ', 6, 6' - tetramethyl azobenzene, then concentrated hydrochloric acid and zinc powder for the one-step method of 2, 2 ', 6, 6' - tetramethyl azobenzene reduction, rearrangement, and through processing and getting the 3, 3 ', 5, 5' - tetramethyl benzidine and its hydrochloride. In the present invention the used raw materials are cheap and easily obtained, operation process is simple and convenient, mild reaction conditions, higher yield. (by machine translation)
Diverse applications of TMB-based sensing probes
Huang, Li,Cao, Ying-Juan,Sun, Xiang-Ying,Liu, Bin,Shen, Jiang-Shan
supporting information, p. 5667 - 5676 (2018/08/17)
Extending the research on 3,3′,5,5′-tetramethylbenzidine (TMB) and its derivatives in analytical chemistry is important, considering that TMB is widely used as an enzyme catalytic substrate. In this work, two TMB derivatives, TMBS and TMBB, were synthesized via a facile and one-step condensation reaction between the -NH2 group of TMB and the -CHO group of salicylaldehyde or benzaldehyde. Because at low pH the two Schiff base compounds can release TMB which can emit strong fluorescence, the probes could show dual-modal signal responses, fluorescence and UV-vis absorption, towards the pH. Practical applications of pH sensing in Chinese rice vinegar and lemon juice samples were successfully demonstrated. On the basis of these findings, a catalytic chromogenic reaction was developed to monitor the pH with the naked eye, too. Furthermore, considering the chemical equilibrium reaction between CO2 and H2O and that glucose oxidase (GOD) can catalyse the dehydrogenation and oxidation reaction of β-d-glucose to produce gluconic acid, both of which can result in lowering the pH values of the two Schiff base systems, highly sensitive and selective dual-modal sensing systems for detecting CO2 and β-d-glucose have also been successfully established. Therefore, the two synthesized TMB derivatives can demonstrate their robust application potential.
Novel cyclic bicarbodiimide compound and preparation method of novel cyclic bicarbodiimide compound
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Paragraph 0039, (2017/08/29)
The invention relates to a novel cyclic bicarbodiimide compound (I) and a preparation method of the novel cyclic bicarbodiimide compound. Compared with monocarbodiimide, the novel cyclic bicarbodiimide compound has a higher molecular weight and a higher melting point; the disadvantage that the monocarbodiimide is easily transferred outward from compounds including polyester, polyurethane and like at high temperature is overcome; the anti-hydrolytic property of a material is improved; meanwhile, the defects that an existing bicarbodiimide compound is easily irregularly curled, easily forms a rubber state at room temperature and is not easy to crystallize and purify are overcome; the anti-hydrolytic effect is good and the bicarbodiimide compound has a good anti-hydrolytic property; the service life of a polyester material is improved; the bicarbodiimide compound can be used as an anti-hydrolytic stabilizer and is used for polyurethane and polyester elastomers, and especially can be used for photovoltaic industry, biodegradable materials and the like. The formula (I) is shown in the description.
New role of graphene oxide as active hydrogen donor in the recyclable palladium nanoparticles catalyzed ullmann reaction in environmental friendly ionic liquid/supercritical carbon dioxide system
Cheng, Jinsheng,Zhang, Gencheng,Du, Jin,Tang, Longhua,Xu, Jingying,Li, Jinghong
experimental part, p. 3485 - 3494 (2011/10/02)
An economical and green pathway: graphene oxide (GO)-supported palladium nanoparticles (Pd NPs) catalyzed a reductive Ullmann reaction of aryl chloride towards biaryl with high conversion and selectivity in ionic liquid (IL)-supercritical carbon dioxide (ScCO2). The combination of IL and ScCO2 provides superior advantages in product separation, catalyst recycling and reuse of the reaction media over traditional organic solvents. Further investigations showed that GO, the novel catalyst support bearing abundant carboxylic, hydroxyl, epoxy and aldehyde groups, can replace the traditional active hydrogen donor readily with much enhanced product separation efficiency. The use of IL, e.g. [hmim][Tf2N], led to obvious improved stability of the Pd NPs, which was helpful for catalyst recycling. Carbon dioxide, a naturally abundant, nonflammable, relatively nontoxic, economical and recyclable "greenhouse" gas, was found to significantly promote the selectivity of the graphene oxide-based Pd NP-catalyzed reductive Ullmann reaction of aryl chloride. Investigations showed that the Pd NP catalyst and IL can be recycled for more than 5 runs without obvious loss of conversion, indicating the economical viability of this process.
An economical, green pathway to biaryls: Palladium nanoparticles catalyzed ullmann reaction in ionic liquid/ supercritical carbon dioxide system
Cheng, Jinsheng,Tang, Longhua,Xu, Jingying
experimental part, p. 3275 - 3286 (2011/02/23)
In this paper, an economical, green pathway involving the palladium nanoparticles (Pd NPs) catalyzed reductive Ullmann reaction of an aryl chloride to afford a biaryl with high conversion and selectivity in an ionic liquid (IL)/supercritical carbon dioxide (ScCO2) system was developed. The combination of IL and ScCO2 provides superior advantages in product separation, catalyst recycling and reuse of reaction media over traditional organic solvents. Further investigations showed that the Bronsted-acidic imidazolium IL {e.g., (1-butyl-3-(sulfobutyl)-imidazolium) hydrogen sulfate, [bmim(HSO3C4)][HSO4]}, can replace the traditional active hydrogen donor readily with much enhanced product separation efficiency, the use of IL also led to an obvious improved stability of the Pd NPs, which was very helpful for catalyst recycling. Carbon dioxide, a naturally abundant, non-flammable, relatively non-toxic, economical and recyclable "greenhouse" gas, was found to significantly promote the selectivity of the Pd NPs-catalyzed aluminium-induced reductive Ullmann reaction of aryl chlorides. Investigations showed that the Pd NPs catalyst and IL can be recycled for at least five runs, indicating the economic viability of this process. Copyright
Functionalized Photoreactive Compounds
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, (2008/12/08)
The present invention concerns functionalized photoreactive compounds of formula (I), that are particularly useful in materials for the alignment of liquid crystals. Due to the adjunction of an electron withdrawing group to specific molecular systems bearing an unsaturation directly attached to two unsaturated ring systems, exceptionally high photosensitivities, excellent alignment properties as well as good mechanical robustness could be achieved in materials comprising said functionalized photoreactive compounds of the invention.
Antimicrobial compositions
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, (2008/06/13)
The present invention relates to an enzymatic method for killing or inhibiting microbial cells or microorganisms, e.g. in laundry, on hard surfaces, in water systems, on skin, on teeth or on mucous membranes. The present invention also relates to the use of said enzymatic composition for preserving food products, cosmetics, paints, coatings, etc.
