Journal of the American Chemical Society p. 14336 - 14347 (2007)
Update date:2022-09-26
Topics:
Nolan, Elizabeth M.
Fischbach, Michael A.
Koglin, Alexander
Walsh, Christopher T.
The present work reveals that four proteins, MceCDIJ, encoded by the MccE492 gene cluster are responsible for the remarkable post-translational tailoring of microcin E492 (MccE492), an 84-residue protein toxin secreted by Klebsiella pneumonaie RYC492 that targets neighboring Gram-negative species. This modification results in attachment of a linearized and monoglycosylated derivative of enterobactin, a nonribosomal peptide and iron scavenger (siderophore), to the MccE492m C-terminus. MceC and MceD derivatize enterobactin by C-glycosylation at the C5 position of a N-(2,3-dihydroxybenzoyl)serine (DHB-Ser) moiety and regiospecific hydrolysis of an ester linkage in the trilactone scaffold, respectively. Mcel and MceJ form a protein complex that attaches C-glycosylated enterobactins to the C-terminal serine residue of both a C10 model peptide and full-length MccE492. In the enzymatic product, the C-terminal serine residue is covalently attached to the C4′ oxygen of the glucose moiety. Nonenzymatic and base-catalyzed migration of the peptide to the C6′ position affords the C6′ glycosyl ester linkage observed in the mature toxin, MccE492m, isolated from bacterial cultures.
View MoreJiangxi Global Natural Spice Co., Ltd.
website:https://www.jxhqxl.com/
Contact:+86-796-8106598
Address:No.89, Wenshan Road,South Industrial Park, Jishui County,Jiangxi Province
CGeneTech (Suzhou, China) Co., Ltd.
Contact:+86-512-62956962
Address:Room 101,Bld C11,218 Xinghu Rd.,Suzhou industrial Park
Shanghai ZaiQi Bio-Tech Co., Ltd.,
Contact:+86-21-5482 4098
Address:Bldg. No.7, No.201 MinYi Rd,Songjiang CaoHeJing High-Tech Park Shanghai 201516 P,R,China
website:http://www.truewingroup.com
Contact:86-311-66699812
Address:NO.600 ZHONGSHAN EAST ROAD SHIJIAZHUANG
Contact:+86-518-81061113
Address:No. 8 Lingzhou Road, Lianyungang, Jiangsu, China
Doi:10.1016/j.tetlet.2018.03.066
(2018)Doi:10.1021/jm070669h
(2007)Doi:10.1021/ja00741a013
(1971)Doi:10.1007/BF02159419
()Doi:10.1016/j.ejmech.2011.04.011
(2011)Doi:10.1016/j.bmc.2014.04.016
(2014)