S-Ribosylhomocysteine analogues with the carbon-5 and sulfur atoms replaced by a vinyl or (fluoro)vinyl unit

Article abstract of DOI:10.1016/j.bmc.2008.03.028

Treatment of the protected ribose or xylose 5-aldehyde with sulfonyl-stabilized fluorophosphonate gave (fluoro)vinyl sulfones. Stannyldesulfonylation followed by iododestannylation afforded 5,6-dideoxy-6-fluoro-6-iodo-d-ribo or xylo-hex-5-enofuranoses. Coupling of the hexenofuranoses with alkylzinc bromides gave 10-carbon ribosyl- and xylosylhomocysteine analogues incorporating a fluoroalkene. The fluoroalkenyl and alkenyl analogues were evaluated for inhibition of Bacillus subtilis S-ribosylhomocysteinase (LuxS). One of the compounds, 3,5,6-trideoxy-6-fluoro-d-erythro-hex-5-enofuranose, acted as a competitive inhibitor of moderate potency (K_I = 96 μM).

Full text of DOI:10.1016/j.bmc.2008.03.028

Available online at www.sciencedirect.com
Bioorganic & Medicinal Chemistry 16 (2008) 5090–5102
S-Ribosylhomocysteine analogues with the carbon-5 and
sulfur atoms replaced by a vinyl or (fluoro)vinyl unit
Stanislaw F. Wnuk,a,* Jennifer Lalama,a,ꢀ Craig A. Garmendia,a
Jenay Robert,a Jinge Zhub and Dehua Peib
aDepartment of Chemistry & Biochemistry, Florida International University, Miami, FL 33199, USA
bDepartment of Chemistry and Ohio State Biochemistry program, The Ohio State University, Columbus, OH 43210, USA
Received 13 January 2008; revised 5 March 2008; accepted 10 March 2008
Available online 14 March 2008
Abstract—Treatment of the protected ribose or xylose 5-aldehyde with sulfonyl-stabilized fluorophosphonate gave (fluoro)vinyl
sulfones. Stannyldesulfonylation followed by iododestannylation afforded 5,6-dideoxy-6-fluoro-6-iodo-D-ribo or xylo-hex-5-enof-
uranoses. Coupling of the hexenofuranoses with alkylzinc bromides gave 10-carbon ribosyl- and xylosylhomocysteine analogues
incorporating a fluoroalkene. The fluoroalkenyl and alkenyl analogues were evaluated for inhibition of Bacillus subtilis S-ribosyl-
homocysteinase (LuxS). One of the compounds, 3,5,6-trideoxy-6-fluoro-D-erythro-hex-5-enofuranose, acted as a competitive inhib-
itor of moderate potency (KI = 96 lM).
ꢀ 2008 Elsevier Ltd. All rights reserved.
1. Introduction
Several substrate analogues of SRH (e.g., 1 and 2) showed
submicromolar inhibition of LuxS.4e,h
S-Adenosyl-L-homocysteine (SAH) is a byproduct of
many methyltransferase reactions and a potent inhibitor
of the methyltransferases. In eukaryotes and some bacte-
ria, detoxification of SAH is mediated by SAH hydrolase
(EC 3.3.1.1), which effects hydrolytic cleavage of SAH to
L-homocysteine (Hcy) and adenosine (Fig. 1).1 Hcy ap-
pears to be a risk factor for coronary artery diseases.2
Alternatively, most bacteria utilize enzyme 50-methylthio-
adenosine (MTA)/SAH nucleosidase (EC 3.2.2.9) to
irreversibly cleave SAH yielding adenine and S-ribosyl-
L-homocysteine (SRH).3 The SRH is then converted to
Hcy and 2,4-dihydroxy-2,3-pentadione (DPD) by a
metalloenzyme S-ribosylhomocysteinase (LuxS).4 DPD5
spontaneously cyclizes and complexes with borate to
form a furanosyl borate diester, which acts as a type 2
autoinducer for bacterial interspecies quorum sensing.6
Since quorum sensing regulates many bacterial behaviors
such as virulence and biofilm formation, LuxS and other
proteins involved in quorum sensing have been proposed
as attractive targets for novel antibacterial drug design.7
We have previously observed that SAH hydrolase is
capable of the addition of water across 50,60 isolated
double bond of adenosine analogues
3 and 4
(Fig. 2).1c,8 The resulting adduct (or its derivative)
caused covalent modification and inactivation8b of the
enzyme, a process which required the catalytic activity
of the enzyme. Since LuxS catalyzes a similar reaction
as SAH hydrolase (i.e., overall elimination of Hcy), we
designed analogues of SRH with the vinyl or halovinyl
moieties incorporated in place of the carbon-5 and sul-
fur atoms (e.g., 5). We envisaged that these ribosyl ana-
logues might serve as mechanistic probes to study the
mechanism of action of LuxS and evaluate the similari-
ties between SAH hydrolase and LuxS. As mentioned
above, LuxS inhibitors may provide a novel class of
antibacterial agents. We now describe the syntheses of
SRH analogues with the carbon-5 and sulfur atoms re-
placed by vinyl or (6-fluoro)vinyl motifs and discuss
their interactions with LuxS enzyme.
Keywords: LuxS enzyme, Negishi coupling; S-Ribosylhomocysteine;
Vinyl fluorides; Vinyl stannanes.
2. Chemistry
*
Corresponding author. Tel.: +1 305 348 6195; fax: +1 305 348
3772; e-mail: wnuk@fiu.edu
Present address: Azopharma, Inc., Miramar, FL, USA.
Our initial plan to prepare compound 5 and its congen-
ers is illustrated in Scheme 1. Treatment of the diacetone
ꢀ
0968-0896/$ - see front matter ꢀ 2008 Elsevier Ltd. All rights reserved.
doi:10.1016/j.bmc.2008.03.028

S. F. Wnuk et al. / Bioorg. Med. Chem. 16 (2008) 5090–5102
5091
H2N
CO2H
S+
H2N
CO2H
S
NH2
N
NH2
N
NH2
N
N
N
N
N
N
N
HO
H2N
CO2H
SH
a
N
N
N
O
O
O
+
SAHH
Methyl
transferases
OH
OH
OH
OH
OH
OH
SAM
SAH
NH2
N
b
N
Pfs
OH
H2N
CO2H
N
H
N
HO
OH
O
–
B(OH)4
HO
B
O
OH
S
O
LuxS
CH3
O
OH
O
HO
HO
CH3
O
HO
O
O
H2O
H2N
CO2H
OH
OH
DPD
AI-2
SRH
SH
Figure 1. Reaction pathways for SAH detoxification in eukaryotes (a) and the majority of bacteria (b). The latter is also utilized by the bacteria to
produce the type 2 autoinducer.
NH2
O
O
the non-stabilized ylides.9b Similarly, Wittig-treatment
of ribo 5-aldehyde 9 gave 11; a nine-carbon analogue
of SRH. Unfortunately, our attempts to add bromine
(CH2Cl2/0 ꢁC) across the double bond of 10 or 11 (as
well as 16) produced a complex mixture which did not
give the desired SRH analogues of type 5 bearing a (6-
bromo)vinyl unit when treated with DBU.10
OH
O
HO
S
5'
S
5
OH
OH
N
H
HO
O
OH
NH2
HO OH
A
2
1
NH2
6
X
6'
Y
O
O
HO2C
OH
X
HO OH
HO OH
In an alternative approach, we attempted a synthesis of
6-bromoalkenyl analogues 5 (X = Br) via Pd-catalyzed
monoalkylation11–13 of the readily available (gem-dibro-
mo)vinyl sugar precursors (e.g., 12 and 13) with the cor-
responding alkylzinc reagents. Thus, dibromolefination
of xylo 5-aldehyde 8 by the Corey–Fuchs procedure14
gave 5-(dibromomethylene)-5-deoxyxylose 12 (81%
from 6; Scheme 2). Analogous treatment of the ribo
5-aldehyde 9 afforded 13.15 Treatment of 12 with 3 equiv
of 4-ethoxy-4-oxobutylzinc bromide in the presence of
Pd(PPh3)4 at 55 ꢁC gave monoalkylated 5,6,7,8,9-penta-
deoxy-a-D-xylo-dec-5(E)-enofuranuronate 14 (18%,
3J5-6 = 15.4 Hz) and dialkylated 18 (48%) products, but
did not yield the desired 6-bromoalkenyl product 15.
Analogous Negishi coupling of 5-(dibromomethylene)-
5-deoxyribose 13 afforded only dialkylated product 19
(54%). Changing catalyst [(Pd2(dba)3)], solvent (THF),
reaction temperature (rt to 60 ꢁC) as well as adding
additives (CuI, tricyclohexylphosphine) did not lead to
the formation of 15 or 17 but instead produced dialky-
lated byproducts 18 and 19 (3–49%) in agreement with
a recent report.13b
3 X = I, Br, Cl, or F; Y = H
4 X = Br;Y = Br or F
5 X = H or halogen
Figure 2. Inhibitors of LuxS enzyme (1 and 2).4e,h 50-Deoxy-50-
(halomethylene)adenosine analogues (3 and 4) which serve as suicide
substrates for SAH hydrolase8 and targeted SRH analogues (5) in
which the sulfur and C5 atoms are replaced by a vinyl unit.
3-O-benzoylglucose 6 or allose 7 with periodic acid
selectively removed the 5,6-O-isopropylidene group.
Subsequent oxidative cleavage of the exposed vicinal
diol9a gave the corresponding 5-aldehydes 8 and 9,
respectively, in high yields (Scheme 1). Wittig olefination
of aldehyde 8 with the ylide derived from commer-
cially available [4-ethoxy-4-oxobutyltriphenylphoshoni-
um bromide provided a complex mixture of products.
Column chromatography yielded protected 5,6,7,
8-tetradeoxy-a-D-xylo-non-5(Z)-enofuranuronate 10 (18%
yield). The stereochemistry was assigned as Z, based
on the magnitude of the coupling constants for olefinic
protons (3J5-6 = 11.1 Hz), and literature precedence for
the Wittig condensations of aliphatic aldehydes with
We next explored stereoselective coupling of the gem-
dihalovinyl sugars containing two different halogens.
We chose 5-deoxy-5-(fluoroiodomethylene) hexenofura-
noses 26 and 27 because the iodo and fluoro substituents
are known to have quite different reactivity towards oxi-
dative–addition in Pd-mediated couplings.11b,16,17 The
precursors 26 and 27 were prepared employing McCar-
thy’s stannyldesulfonylation methodology.18,19 Thus,
treatment of the xylo aldehyde 8 with the enolate gener-
ated from the sulfonyl-stabilized fluorophosphonate20
O
O
O
O
a
O
b
O
O
O
X
X
X
O
O
O
O
O
O
Y
Y
Y
6 X = OBz, Y = H
7 X = H, Y = OBz
8 X = OBz, Y = H
9 X = H, Y = OBz
10 X = OBz, Y = H
11 X = H, Y = OBz
Scheme 1. Reagent: (a) H5IO6/EtOAc; (b) Ph3PCH2CH2CH2CO2Et/
HMDS/THF.

5092
S. F. Wnuk et al. / Bioorg. Med. Chem. 16 (2008) 5090–5102
Br
Br
N-iodosuccinimide (NIS) quantitatively afforded 6-flu-
O
O
O
oro-6-iodo-xylo-hex-5-enofuranoses 26 with retention
of the E/Z configuration. The ribo analogue 27 (E/Z,
3:2; 57% overall yield from 9) was similarly prepared.
The isomeric ratio for the fluorinated sugars could be
X
a
X
O
O
O
O
Y
Y
8 X = OBz, Y = H
12 X = OBz, Y = H
9 X = H, Y = OBz
13 X = H, Y = OBz
3
distinguished by the magnitude of the JF-H5 in the
NMR spectra.
b
O
O
Pd-mediated cross-coupling of the xylo analogue 26 (E/
Z, 4:1) with 2 equiv of 4-ethoxy-4-oxobutylzinc bro-
mide resulted in selective consumption of (E)-26 to af-
ford (Z)-29 in 61% isolated yield or 76% based on
consumption of (E)-26 (Scheme 4). A small amount
of (E)-29 was also isolated, although monocoupling
with gem-dihalovinyl substrates is considered to be
trans selective.12,13b,16 Similar monoalkylation of the
ribo analogue 27 (E/Z, 3:2) with BrZn(CH2)3COOEt
yielded (Z)-30 [54%, 90% based on the conversion of
(E)-27]21 and (E)-30 [12%, 30% from (Z)-27]. Coupling
of the (iodo)vinyl (E)-28, prepared as depicted in
Scheme 3 (9 ! 22 ! 25 ! 28), with BrZn(CH2)3COO-
Et gave the unfluorinated analogue (E)-16 (56%) with
the retention of configuration. Treatment of (Z)-29
with NH3/MeOH removed the benzoyl group and con-
verted the ethyl ester into a methyl ester (Z)-31 (74%).
Subsequent removal of the isopropylidene group with
aqueous trifluoroacetic acid (TFA) at 0 ꢁC gave (Z)-
33 (61%; a/b, 1:1). Successive treatment of (Z)-30 with
NH3/MeOH followed by TFA/H2O gave (Z)-34 (52%
overall yield; a/b, 3:7); a 10-carbon 6-fluoroalkenyl ana-
logue of SRH.
O
X
O
O
O
O
+
X
O
O
O
O
O
Z
Y
Y
18 X = OBz, Y = H
19 X = H, Y = OBz
14 X = OBz, Y = H, Z = H
15 X = OBz, Y = H, Z = Br
16 X = H, Y = OBz, Z = H
17 X = H, Y = OBz, Z = Br
Scheme 2. Reagents: (a) PPh3/CBr4; (b) BrZn(CH2)3CO2Et/Pd(PPh3)
4/benzene/D.
PhSO2
O
O
X
O
X
a
Z
O
O
O
O
Y
Y
20 X = OBz, Y = H, Z = F
21 X = H, Y = OBz, Z = F
22 X = H, Y = OBz, Z = H
8 X = OBz, Y = H
9 X = H, Y = OBz
b
I
Bu3Sn
c
O
O
X
Z
Z
X
O
O
O
Y
O
Y
The 5,6-dideoxy-6-fluorohex-5-enofuranoses 42 and 43,
depurinated analogues of 3 (X = F), were synthesized
by protiodestannylation of the (fluoro)vinyl stannanes
23 and 24. Thus, treatment of 23 (E/Z, 7:3) with NH3/
MeOH at 25 ꢁC resulted in the removal of 3-O-benzoyl
group to give 36 (Scheme 5). However, prolonged heat-
ing of 36 (or 23) with NH3/MeOH at 65 ꢁC for 48 h ef-
fected protiodestannylation to yield a separatable
mixture of (E)-39 (29%) and (Z)-39 (48%). Treatment
of (E)-39 with TFA/H2O at 0 ꢁC gave (E)-42 (a/b,
ꢀ1:1). Analogous debenzoylation and protiodestannyla-
tion of 24 (E/Z, 1:1) with NH3/MeOH yielded (E)-40
(32%) and (Z)-40 (26%). Acid-catalyzed removal of the
isopropylidene group in (E)-40 gave 5,6-dideoxy-6-flu-
oro-D-ribo-hex-5-enofuranose (E)-43 (67%; a/b, ꢀ1:4).
Alternatively, concomitant protiodestannylation and
removal of acetone unit in 36 or 37 with TFA also affor-
ded 42 and 43.
26 X = OBz, Y = H, Z = F
27 X = H, Y = OBz, Z = F
28 X = H, Y = OBz, Z = H
23 X = OBz, Y = H, Z = F
24 X = H, Y = OBz, Z = F
25 X = H, Y = OBz, Z = H
Scheme 3. Reagents and conditions: (a) PhSO2CHFPO(OEt)2 or
PhSO2CH2PO(OEt)2/LHMDS/ THF/ꢁ78 ꢁC; (b) Bu3SnH/AIBN/tol-
uene/85 ꢁC; (c) NIS/CH2Cl2.
gave (fluoro)vinyl sulfones 20 (E/Z, 7:3; 76%; Scheme 3).
The stereoselective radical-mediated stannyldesulfonyla-
tion of 20 with Bu3SnH produced (fluoro)vinyl stann-
anes 23 (E/Z, 7:3; 95%). Iododestannylation of 23 with
I
O
O
O
a
X
Z
O
X
O
Z
O
O
O
Y
Y
29 X = OBz, Y = H, Z = F
30 X = H, Y = OBz, Z = F
16 X = H, Y = OBz, Z = H
26 X = OBz, Y = H, Z = F
27 X = H, Y = OBz, Z = F
28 X = H, Y = OBz, Z = H
The 3,5,6-trideoxy 6-fluorohex-5-enofuranose 44, which
lacks a hydroxyl group at C3 and therefore cannot par-
ticipate in the second enolization step of the LuxS-cata-
lyzed reaction,4b was also prepared. Thus, oxidation of
b
9a
the diacetone 3-deoxyglucose22 with H5IO6
and
O
X
O
O
O
OH
c
X
in situ treatment of the rather unstable 3-deoxyribose
5-aldehyde with the enolate generated from the sulfo-
nyl-stabilized fluorophosphonate20 gave the (flu-
oro)vinyl sulfones 35 (48%; E/Z, 2:1). Subjection of 35
to the stannyldesulfonylation/protiodestannylation18b
sequence afforded 3-deoxy (6-fluoro)vinyl sugar 41,
which was deprotected to yield 44 (12% from 35). Alter-
O
O
O
F
F
O
OH
Y
Y
31 X = OH, Y = H
32 X = H, Y = OH
33 X = OH, Y = H
34 X = H, Y = OH
Scheme 4. Reagents: (a) BrZn(CH2)3CO2Et/Pd(PPh3)4/benzene/D;
(b) NH3/MeOH; (c) TFA/H2O.

S. F. Wnuk et al. / Bioorg. Med. Chem. 16 (2008) 5090–5102
5093
Bu3Sn
Z
A
1
0.8
0.6
0.4
0.2
0
O
X
O
X
a
or
b
[SRH] = 20 μM
[SRH] = 7 μM
F
F
O
O
O
O
Y
Y
23 X = OBz, Y = H, Z = Bu3Sn
24 X = H, Y = OBz, Z = Bu3Sn
35 X = Y = H, Z = PhSO2
36 X = OH, Y = H
37 X = H, Y = OH
38 X = Y = H
d
c
F
F
O
X
O
X
0.0
0.50
1.0
[I] (mM)
1.5
2.0
2.5
d
OH
O
O
OH
Y
Y
1200
B
42 X = OH, Y = H
43 X = H, Y = OH
44 X = Y = H
39 X = OH, Y = H
40 X = H, Y = OH
41 X = Y = H
1000
800
600
400
200
0
Scheme 5. Reagents and conditions: (a) NH3/MeOH/25 ꢁC; (b)
Bu3SnH/AIBN/toluene/85 ꢁC; (c) NH3/MeOH/65 ꢁC or NH3/MeOH/
CsF/65 ꢁC; (d) TFA/H2O.
natively, treatment of vinyl stannanes 38 with TFA ef-
fected simultaneous protiodestannylation and removal
of the acetone unit to give 44 (23% from 35; E/Z,
ꢀ1:3, a/b ꢀ1:4).
-0.10 -0.050 0.0
0.050 0.10
0.15
1/[S] (μM-1)
Figure 3. Inhibition of Co2+-substituted B. subtilis LuxS by compound
44. (A) Plot of remaining LuxS activity (relative to that in the absence
of inhibitor) as a function of [I]. (B) Lineweaver–Burke plot of data
from part A to show the competitive inhibition mode.
3. Inhibition of LuxS
The (6-fluoro)vinyl xylo- (42) and ribo-hexofuranoses
(43) and their 3-deoxy analogue 44 as well as (6-flu-
oro)vinyl xylo- and ribo-decofuranoses (33 and 34) were
evaluated4h as potential inhibitors of Bacillus subtilis
S-ribosylhomocysteinase (LuxS). Compound 44 exhib-
ited competitive inhibition of moderate potency, with
a KI value of 96 3 lM (Fig. 3). None of the other
compounds showed significant inhibition under the
assay conditions.
dried by reflux over and distillation from CaH2 under
an argon atmosphere except THF (K/benzophenone).
TLC was performed on Merck kieselgel 60-F254 with
MeOH/CHCl3 (1:9) and EtOAc/MeOH (95:5) as devel-
oping systems, and products were detected with
254 nm light or by visualization with Ce(SO4)2/
(NH4)6Mo7O24Æ4H2O/H2SO4/H2O reagent. Merck kie-
selgel 60 (230–400 mesh) was used for column chroma-
tography. Elemental analyses were determined by
Galbraith Laboratories, Knoxville, TN.
4. Summary and conclusions
We have developed synthesis of six-, nine-, and 10-car-
bon analogues of ribosyl- and xylosylhomocysteines in
which the carbon-5 and sulfur atoms are replaced by a
vinyl or (fluoro)vinyl unit. These fluoroalkenyl and alke-
nyl analogues of SRH were synthesized employing either
the Wittig reaction or Pd-catalyzed coupling routes.
They were evaluated against B. subtilis S-ribosylhomo-
cysteinase (LuxS). Only 3,5,6-trideoxy-6-fluoro-D-ery-
thro-hex-5-enofuranose acted as competitive inhibitor
of moderate potency with KI = 96 lM.
5.1. Ethyl 3-O-benzoyl-5,6,7,8-tetradeoxy-1,2-O-isopro-
pylidene-a-D-xylo-non-5(Z)-enofuranuronate (10)
Step (a). H5IO6 (150 mg, 0.66 mmol) was added to a
stirred solution of 6 (200 mg, 0.55 mmol) in dried
EtOAc at ambient temperature. A precipitate appeared
within the first 5 min and the resulting solution was stir-
red for 90 min. The precipitate was filtered off and was
washed with EtOAc (2· 5 mL). The combined organic
layer was washed with NaHCO3/H2O (10 mL), NaCl/
H2O (10 mL), dried (Na2SO4), and evaporated to
yield 3-O-benzoyl-1,2-O-isopropylidene-a-D-xylo-pentodi-
aldo-1,4-furanose (8; 160 mg, 95%; approximately 90%
5. Experimental
1
1
1H (Me4Si) NMR spectra were determined with solution
in CDCl3 at 400 or 600 MHz, 13C (Me4Si) at 100.6 MHz
and 19F (CFCl3) at 376.5 MHz unless otherwise noted.
Mass spectra (MS) were obtained by atmospheric pres-
sure chemical ionization (APCI) and HRMS by electron
impact techniques unless otherwise noted. Reagent
grade chemicals were used as received. Solvents were
pure based on H NMR): H NMR d 1.35 and 1.48
(2· s, 2 · 3, 2· CH3), 4.76 (d, J4-3 = 3.2 Hz, 1, H4),
4.88 (d, J2-1 = 3.1 Hz, 1, H2), 5.77 (d, J1-2 = 3.1 Hz, 1,
H1), 6.18 (d, J3-4 = 3.3 Hz, 1, H3), 7.42–8.01 (m, 5,
Ar), 9.78 (s, 1, H5). Step (b). LHMDS (1 M/THF;
0.69 mL, 0.69 mmol) was added dropwise to a stirred
solution of Ph3PCH2CH2CH2CO2Et/Br (314 mg,

5094
S. F. Wnuk et al. / Bioorg. Med. Chem. 16 (2008) 5090–5102
0.69 mmol) in anhydrous THF (4 mL) in a flame-dried
flask under N2 at ambient temperature. After 15 min,
a solution of the crude, preferentially freshly prepared,
aldehyde 8 (160 mg of the material from Step (a)) in
THF (2 mL) was added via syringe and stirring was con-
tinued overnight. EtOAc (30 mL) and NaHCO3/H2O
(10 mL) were added and the separated organic was
washed with NaCl/H2O (10 mL), dried (Na2SO4), and
evaporated. Column chromatography (10 ! 30% hex-
24.5 mmol) in dried CH2Cl2 (100 mL) at 0 ꢁC (ice-bath)
for 30 min followed by stirring at ambient temperature
under N2 for 3 h] was added to the solution of freshly
prepared aldehyde 8 [prepared as described for 10 (Step
(a) from 6 (4.68 g, 12.9 mmol) and dried for 1 h under
vacuum prior to use] in CH2Cl2 (75 mL). After stirring
for 14 h at ambient temperature, the reaction mixture
was partitioned (NaHCO3/H2O/CHCl3), and the organ-
ic layer was washed (H2O, brine), dried (MgSO4), and
the volatiles were evaporated. Column chromatography
(15 ! 25% EtOAc/hexane) gave 12 (4.68 g, 81% overall
1
anes/EtOAc) gave 10 (39 mg, 18%) as an oil: H NMR
d 1.24 (t, J = 7.2 Hz, 3, CH3), 1.37 and 1.62 (2· s,
1
2 · 3, 2· CH3), 2.41 (t, J8-7/7 = 6.9 Hz, 2, H8/80), 2.50
from 6) as a solidifying viscous oil: H NMR d1.34 and
0
(‘q’, J7-6/8/8 = 7.3 Hz, 2, H7/70), 4.15 (q, J = 7.1 Hz, 2,
CH2), 4.71 (d, J2-1 = 3.8 Hz, 1, H2), 5.22 (dd,
1.58 (2· s, 2 · 3, 2· CH3), 4.68 (d, J2-1 = 3.7 Hz, 1, H2),
5.04 (dd, J4-5 = 7.6 Hz, J4-3 = 3.0 Hz, 1, H4), 5.54 (d, J3-4 =
0
J4-5 = 7.7 Hz, J4-3 = 2.8 Hz, 1, H4), 5.46 (d, J3-4
2.8 Hz, H3), 5.58 (dd, J5-6 = 11.1 Hz, J5-4 = 7.9 Hz, 1,
0
H5), 5.68 (dt, J6-5 = 11.1 Hz, J6-7/7 = 7.1 Hz, 1, H6),
=
3.0 Hz, 1, H3), 6.00 (d, J1-2 = 3.7 Hz, 1, H1), 6.60 (d,
J5-4 = 7.6 Hz, 1, H5), 7.48 (t, J = 7.6 Hz, 2 Ar), 7.60
(tt, J = 1.3, 7.6 Hz, 1 Ar), 8.02 (‘dd’, J = 1.4, 7.7 Hz, 2
Ar); 13C NMR d 24.96 and 25.51 (CMe2), 75.71 (C3),
78.18 (C4), 82.04 (C2), 93.26 (C6), 103.30 (C1), 111.26
(CMe2), 127.31 (Bz), 127.73 (Bz), 128.44 (Bz), 130.49
(C5), 132.37 (Bz), 163.81 (Bz); MS m/z 451 (5, MH+
[81Br2]), 449 (10, MH+ [81/79Br2]), 447 (5, MH+ [79Br2]).
6.05 (d, J1-2 = 3.7 Hz, 1, H1), 7.48–8.04, (m, 5, Ar);
13C NMR d 14.62 (CH3), 24.08 (C7), 26.62 and 27.19
(CMe2), 34.23 (C8), 60.90 (CH2), 75.54 (C2), 78.59
(C3), 84.20 (C4), 105.02 (C1), 112.47 (CMe2), 123.90
(C6), 128.91 (Bz), 129.78 (Bz), 130.15 (Bz), 133.85
(Bz), 134.39 (C5), 165.64 (Bz), 173.09 (C9); MS m/z
391 (100, MH+). HRMS (AP-ESI) m/z calcd for
C21H26O7Li (MLi+) 397.1839; found: 397.1833.
5.4. Ethyl 3-O-benzoyl-5,6,7,8,9-pentadeoxy-1,2-O-iso-
propylidene-a-D-xylo-dec-5(E)-enofuranuronate (14) and
ethyl 3-O-benzoyl-6-[3-(ethoxycarbonyl)propyl]-5,6,7,8,9-
pentadeoxy-1,2-O-isopropylidene-a-D-xylo-dec-5-eno-
furanuronate (18)
5.2. Ethyl 3-O-benzoyl-5,6,7,8-tetradeoxy-1,2-O-isopro-
pylidene-a-D-ribo-non-5(Z)-enofuranuronate (11)
Step (a). Oxidation of 7 (200 mg, 0.55 mmol) with H5IO6
(150 mg, 0.66 mmol)asdescribedfor10, gave3-O-benzoyl -
1,2-O-isopropylidene-a-D-ribo-pentodialdo-1,4-furanose
(9; 145 mg, 85%; approximately 90% pure, 1H NMR): 1H
NMR d1.39 and 1.61 (2· s, 2 · 3, 2· CH3), 4.64 (dd, J4-5
= 2.2 Hz, J4-3 = 9.2 Hz, 1, H4), 5.01 (t, J2-1/3 = 4.2 Hz, 1,
H2), 5.13 (dd, J3-4 = 9.2 Hz, J3-2 = 4.6 Hz, 1, H3), 6.02
(d, J1-2 = 3.4 Hz, 1, H1), 7.48–8.03 (m, 5, Ar), 9.77 (d,
J5-4 = 2.2 Hz, 1, H5). Step (b). Treatment of the crude 9
Pd[P(Ph)3]4 (22 mg, 0.014 mmol) was added to a stirred
solution of 12 (42 mg, 0.094 mmol) in anhydrous benzene
(3 mL) in a flame-dried flask under N2 at ambient tem-
perature. After 2 min, 4-ethoxy-4-oxobutylzinc bromide
(0.5 M/THF; 0.56 mL, 129 mg, 0.28 mmol) was added
and the resulting mixture was heated at 55 ꢁC for 6 h.
The reaction mixture was cooled down to ambient tem-
perature and was partitioned between EtOAc (30 mL)
and NaHCO3/H2O (10 mL). The separated organic layer
was washed with H2O (10 mL), NaCl/H2O (10 mL),
dried (Na2SO4), and evaporated. Column chromatogra-
phy (10 ! 30% EtOAc/hexanes) gave recovered 12
(7 mg, 13%), 14 (7 mg, 18%), and 18 (19 mg, 48%). Com-
(145 mg)
with
Ph3P(CH2)3CO2Et/Br
0.60 mmol) and LHDMS (1 M/THF; 0.60 mmol,
(275 mg,
1
0.60 mL) as described for 10, gave 11 (18 mg, 12%): H
NMR d 1.26 (t, J = 7.1 Hz, 3, CH3), 1.36 and 1.62 (2· s,
1
2 · 3, 2· CH3), 2.38 (t, J8-7/7 = 8.2 Hz, 2, H8/80),
pound 14 had: H NMR d 1.23 (t, J = 7.1 Hz, 3, CH3),
0
2.46–2.55 (m, 1, H7), 2.55–2.67 (m, 1, H70), 4.15
(q, J = 7.1 Hz, 2, CH2), 4.74 (dd, J3-4 = 9.1 Hz,
J3-2 = 4.8 Hz, 1, H3), 4.96 (‘t’, J2-1/3 = 4.3 Hz, 1, H2),
5.20 (t, J4-3/5 = 8.7 Hz, 1, H4), 5.50 (ddt, J5-6 = 10.9 Hz,
0
1.28 and 1.58 (2· s, 2 · 3, 2· CH3), 1.68 (quint, J8-7/7 /9/9
0
0
= 7.5 Hz, 2, H8/80), 2.07 (‘q’, J7-6/8/8 = 7.0 Hz, 2, H7/
0
70), 2.23 (t, J9-8/8 = 7.4 Hz, 2, H9/90), 4.15 (q,
0
J = 7.1 Hz, 2, CH2), 4.70 (d, J2-1 = 3.7 Hz, 1, H2), 4.86
(dd, J4-5 = 7.1 Hz, J4-3 = 2.8 Hz, 1, H4), 5.42 (d,
J3-4 = 2.7 Hz, 1, H3), 5.56 (dd, J5-6 = 15.4 Hz,
J5-4 = 8.7 Hz, J5-7/7
=
1.0 Hz, 1, H5), 5.72 (dt,
0
J6-5 = 10.9 Hz, J6-7/7 = 7.1 Hz, 1, H6), 5.93 (d,
J1-2 = 3.8 Hz, 1, H1), 7.48–8.04, (m, 5, Ar); 13C NMR d
14.30 (CH3), 24.01 (C7), 26.99 and 27.04 (CMe2), 34.53
(C8), 60.89 (CH2), 73.39 (C4), 77.34 (C2), 77.56 (C3),
104.61 (C1), 113.40 (CMe2), 127.26 (C5), 128.90 (Bz),
129.80 (Bz), 130.28 (Bz), 133.80 (Bz), 135.14 (C6),
166.25 (Bz), 173.06 (C9). HRMS (AP-ESI) m/z calcd for
C21H26O7Li (MLi+) 397.1839; found: 397.1828.
0
J5-4 = 7.3 Hz, 1, H5), 5.92 (dt, J6-5 = 15.4 Hz, J6-7/7
= 6.9 Hz, 1, H6), 6.03 (d, J1-2 = 3.7 Hz, 1, H1), 7.46–
8.05, (m, 5, Ar); 13C NMR d 14.59 (CH3), 23.34 (C8),
26.69 and 27.16 (CMe2), 30.10 (C7), 34.05 (C9), 60.74
(CH2), 75.90 (C2), 81.58 (C3), 85.44 (C4), 105.00 (C1),
112.52 (CMe2), 128.35 (C5), 130.17 (Bz), 130.65 (Bz),
132.47 (Bz), 133.60 (Bz), 135.50 (C6), 165.89 (Bz),
173.74 (C10); MS m/z 405 (100, MH+). Compound 18
1
5.3. 3-O-Benzoyl-5,6-dideoxy-6,6-dibromo-1,2-O-isopro-
pylidene-a-D-xylo-hex-5-enofuranose (12)
had: H NMR d 1.21 (t, J = 7.1 Hz, 3, CH3), 1.29 (t,
J = 7.1 Hz, 3, CH3), 1.36 and 1.61 (2· s, 2 · 3, 2· CH3),
1.69 (quint, J = 7.5 Hz, 2H), 1.71–1.80 (m, 2H), 2.05 (t,
J = 7.5 Hz, 2H), 2.10–2.26 (m, 4H), 2.33 (t, J = 7.2 Hz,
2H), 4.09 (q, J = 7.1 Hz, 2, CH2), 4.18 (q, J = 7.1 Hz,
(Dibromomethylene)triphenylphosphorane [generated
in situ by stirring CBr4 (8.09 g, 24.5 mmol), Ph3P
(6.46 g, 24.5 mmol) and activated Zn (dust; 1.60 g,
2, CH2), 4.70 (d, J2-1 = 3.8 Hz, 1, H2), 5.14 (dd, J4-5
=

S. F. Wnuk et al. / Bioorg. Med. Chem. 16 (2008) 5090–5102
5095
8.7 Hz, J4-3 = 2.9 Hz, 1, H4), 5.35 (d, J5-4 = 8.4 Hz, 1,
H5), 5.41 (d, J3-4 = 2.8 Hz, 1, H3), 6.02 (d, J1-2
nyl)methylphosphonate20 (260 mg, 0.84 mmol) in
anhydrous THF (8 mL) in a flame-dried flask under
N2 at ꢁ78 ꢁC. After 30 min, a solution of 8 (265 mg,
0.82 mmol) in THF (4 mL) was added and stirring
was continued for 1.5 h. EtOAc (30 mL) and NH4Cl/
H2O (10 mL) were added and the reaction mixture
was allowed to warm to ambient temperature. The sep-
arated organic layer was washed with NaHCO3/H2O
(10 mL), NaCl/H2O (10 mL), dried (Na2SO4), and
evaporated. Column chromatography (10 ! 30%
EtOAc/hexanes) gave 20 (166 mg, 76%; E/Z, 7:3) as
inseparable mixture of isomers: HRMS (AP-ESI) m/z:
calcd for C22H22FO7S (MH+) 449.1065; found:
449.1071. 19F NMR d ꢁ110.25 (d, JF-H5 = 18.8 Hz,
0.30F, Z), ꢁ119.30 (d, JF-H5 = 32.1 Hz, 0.70F, E).
=
3.8 Hz, 1, H1), 7.47–8.06, (m, 5, Ar); 13C NMR d 14.60
(CH3), 14.65 (CH3), 26.65 and 27.15 (CMe2), 23.32 and
23.84 (C8/80), 30.53 and 36.22 (C7/70), 34.04 and 34.08
(C9/90), 60.59 (CH2), 60.75 (CH2), 75.90 (C4), 78.71
(C3), 84.24 (C2), 104.84 (C1), 112.36 (CMe2), 119.09
(C5), 146.90 (C6), 128.92 (Bz), 128.92 (Bz), 129.81 (Bz),
130.17 (Bz), 133.83 (Bz), 165.70 (Bz), 173.62 and 173.75
(C10/100); MS m/z 519 (100, MH+).
5.5. Ethyl 3-O-benzoyl-5,6,7,8,9-pentadeoxy-1,2-O-iso-
propylidene-a-D-ribo-dec-5(E)-enofuranuronate (16)
Treatment (55 ꢁC, 3 h) of 28 (E; 20 mg, 0.048 mmol) with
4-ethoxy-4-oxobutylzinc bromide (0.5 M/THF; 0.19 mL,
65 mg, 0.096 mmol) as described for 14/18 gave 16 (11 mg,
1
Compound (E)-20 had: H NMR d 1.35 and 1.56 (2·
s, 2 · 3, 2· CH3), 4.73 (d, J2-1 = 3.7 Hz, 1, H2), 5.23
(dt, J4-5 = 7.4 Hz, J4-3/F = 2.3 Hz, 1, H4), 5.49 (d,
J3-4 = 3.1 Hz, 1, H3), 6.03–6.05 (m, 1, H1), 6.43 (dd,
J5-F = 32.4 Hz, J5-4 = 7.2 Hz, 1, H5), 7.48–8.03 (m, 10,
Ar); 13C NMR d 26.55 and 27.10 (CMe2), 73.78 (C4),
78.21 (C3), 83.77 (C2), 105.31 (C1), 113.13 (CMe2),
1
56%): H NMR d 1.24 (t, J = 7.1 Hz, 3, CH3), 1.35 and
0
0
1.59 (2· s, 2 · 3, 2· CH3), 1.72 (quint, J8-7/7 /9/9 = 7.4 Hz,
2, H8/80), 2.13 (‘q’, J7-6/8/8 = 7.2 Hz, 2, H7/70), 2.28 (t,
0
J9-8/8 = 7.6 Hz, 2, H9/90), 4.10 (q, J = 7.1 Hz, 2, CH2),
0
4.65 (dd, J4-5 = 7.6 Hz, J4-3 = 8.9 Hz, 1, H4), 4.74 (dd,
J3-4 = 9.2 Hz, J3-2 = 4.6 Hz, 1, H3), 4.96 (t, J2-1/3
= 4.3 Hz, 1, H2), 5.53 (dd, J5-6 = 15.4 Hz, J5-4 = 7.3 Hz,
1, H5), 5.89 (d, J1-2 = 4.0 Hz, 1, H1), 5.91 (dt, J6-5
2
112.10 (d, J5-F = 3.3 Hz, C5), 128.99 (Ph), 129.08
(Bz), 129.13 (Bz), 129.79 (Ph), 129.94 (Ph), 130.12
(Ph), 130.17 (Bz), 134.25 (Bz), 135.02 (Ph), 156.00 (d,
1J6-F = 300.0 Hz, C6), 165.36 (Bz). Compound (Z)-20
0
= 15.8 Hz, J6-7/7 = 6.8 Hz, 1, H6), 7.46–8.05, (m, 5, Ar);
13C NMR d 14.62 (CH3), 24.40 (C8), 26.92 and 27.00
(CMe2), 31.99 (C7), 33.89 (C9), 60.65 (CH2), 76.92 (C2),
77.65 (C3), 78.60 (C4), 104.37 (C1), 113.35 (CMe2),
127.04 (C5), 128.85 (Bz), 129.83 (Bz), 130.28 (Bz),
133.76 (Bz), 136.28 (C6), 166.29 (Bz), 173.84 (C10); MS
m/z 405 (100, MH+).
had: H NMR d 1.38 and 1.69 (2· s, 2 · 3, 2· CH3),
1
4.76 (d, J2-1 = 3.7 Hz, 1, H2), 5.68 (d, J3-4 = 2.8 Hz, 1,
H3), 5.99 (dd, J5-F = 19.3 Hz, J5-4 = 8.6 Hz, 1, H5),
6.05–6.07 (m, 1, H1), 6.07–6.09 (m, 1, H4), 7.48–8.03
(m, 10, Ar); 13C NMR d 26.98 and 27.32 (CMe2),
3
73.15 (d, J4-F = 10.14 Hz, C4), 79.06 (C3), 83.93
2
(C2), 105.37 (C1), 113.35 (CMe2), 114.10 (d, J5-F
5.6. Ethyl 3-O-benzoyl-5,6,7,8,9-pentadeoxy-6-[3-(ethoxy-
carbonyl)propyl]-1,2-O-isopropylidene-a-D-ribo-dec-5-eno-
furanuronate (19)
= 15.0 Hz, C5), 128.99 (Ph), 129.08 (Bz), 129.13 (Bz),
129.79 (Ph), 129.94 (Ph), 130.12 (Ph), 130.17 (Bz),
134.25 (Bz), 135.02 (Ph), 155.58 (d, J6-F = 292.3 Hz,
1
C6), 165.36 (Bz). Note: Freshly prepared aldehyde 8,
dried under vacuum for 2 h at ambient temperature
prior to the use, gave the best results.
Treatment of 1315 (42 mg, 0.094 mmol) with Pd[P(Ph)3]4
(22 mg, 0.014 mmol) and 4-ethoxy-4-oxobutylzinc bro-
mide (0.56 mL, 129 mg, 0.28 mmol) as described for
14/18 gave 19 (26 mg, 54%): 1H NMR d 1.22 (t,
J = 7.1 Hz, 6, 2· CH3), 1.37 and 1.62 (2· s, 2 · 3, 2·
CH3), 1.75 (quint, J = 7.5 Hz, 4H), 2.11 (t, J = 7.0 Hz,
2H), 2.24 (t, J = 9.0 Hz, 2H), 2.26 (t, J = 9.0 Hz, 2H),
2.32 (t, J = 7.2 Hz, 2H), 4.10 (q, J = 7.1 Hz, 2, CH2),
4.16 (q, J = 7.1 Hz, 2, CH2), 4.74 (dd, J3-4 = 9.0 Hz,
J3-2 = 4.8 Hz, 1, H3), 4.95 (t, J2-3 = 4.3 Hz, 1, H2),
5.01 (t, J4-5 = 9.0 Hz, 1, H4), 5.26 (d, J5-4 = 8.9 Hz, 1,
H5), 5.89 (d, J1-2 = 3.9 Hz, 1, H1), 7.40–8.10 (m, 5,
Ar); 13C NMR d 14.55 (CH3), 14.61 (CH3), 21.48 (C8/
80), 23.11 and 27.03 (CMe2), 30.08 and 30.12 (C7/70),
32.34 and 34.04 (C9/90), 60.73 (CH2), 60.83 (CH2),
73.86 (C4), 78.71 (C3), 84.24 (C2), 104.37 (C1), 113.28
(CMe2), 122.65 (C5), 128.92 (Bz), 128.92 (Bz), 129.81
(Bz), 130.17 (Bz), 133.83 (Bz), 143.90 (C6), 165.70
(Bz), 170.57 and 171.62 (C10/100); MS m/z 519 (100,
MH+).
5.8. (E/Z)-3-O-Benzoyl-5,6-dideoxy-6-fluoro-1,2-O-isopro-
pylidene-6-phenylsulfonyl-a-D-ribo-hex-5-enofuranose (21)
Treatment of 9 (200 mg, 0.68 mmol) with diethyl
fluoro(phenylsulfonyl)methylphosphonate20
(212 mg,
0.68 mmol) and LHMDS (0.68 mL, 114 mg, 0.68 mmol)
as described for 20 gave 21 (216 mg, 71%; E/Z, 6:4):
HRMS (AP-ESI) m/z: calcd for C22H22FO7S (MH+)
449.1065; found: 449.1069. 19F NMR d ꢁ108.98 (d,
JF-H5 = 22.6 Hz, 0.40F, Z), ꢁ121.25 (d, JF-H5
=
1
30.1 Hz, 0.60F, E). Compound (E)-21 had: H NMR d
1.28 and 1.38 (2· s, 2 · 3, 2· CH3), 4.85 (dd, J3-4
9.0 Hz, J3-2 = 4.7 Hz, 1, H3), 5.00 (‘t’, J2-1/3 = 4.5 Hz, 1,
H2), 5.10 (t, J4-3/5 = 8.2 Hz, 1, H4), 5.94 (d, J1-2
=
=
3.7 Hz, 1, H1), 6.37 (dd, J5-F = 31.3 Hz, J5-4= 8.3 Hz, 1,
H5), 7.44–8.20 (m, 10, Ar); 13C NMR d 26.86 and
26.93 (CMe2), 71.36 (d, J4-F = 2.2 Hz, C4), 76.64 (d,
3
4J3-F = 1.8 Hz, C3), 77.54 (C2), 104.96 (C1), 114.10
(CMe2), 114.22 (d, J5-F = 3.1 Hz, C5), 128.88 (Ph),
2
5.7. (E/Z)-3-O-benzoyl-5,6-dideoxy-6-fluoro-1,2-O-isopro-
pylidene-6-phenylsulfonyl-a-D-xylo-hex-5-enofuranose (20)
129.13 (Bz), 129.18 (Bz), 129.88 (Ph), 130.28 (Ph),
=
1
133.98 (Bz), 135.10 (Ph), 136.96 (Ph), 156.91 (d, J6-F
1
LHMDS (0.84 mL, 140 mg, 0.84 mmol) was added
dropwise to a solution of diethyl fluoro(phenylsulfo-
306.0 Hz, C6), 165.96 (Bz). Compound (Z)-21 had: H
NMR d 1.28 and 1.38 (2· s, 2 · 3, 2· CH3), 4.84 (dd,

5096
S. F. Wnuk et al. / Bioorg. Med. Chem. 16 (2008) 5090–5102
J3-4 = 9.2 Hz, J3-2 = 4.6 Hz, 1, H3), 5.01 (‘t’, J2-1/3
4.6 Hz, 1, H2), 5.86 (dd, J5-F = 19.8 Hz, J5-4 = 9.9 Hz,
=
(m, 1, H4), 5.98 (d, J1-2 = 3.8 Hz, 1, H1 ), 6.02 (dd, J5-F =
34.3 Hz, J5-4 = 9.2 Hz, 1, H5), 7.47–8.06 (m, 5, Ar); 13C
NMR d 10.33 (Bu), 11.15 (Bu), 17.90 (Bu), 27.42 and
27.54 (CMe2), 28.24 (Bu), 74.73 (d, J4-F = 22.2 Hz,
H5), 5.96 (d, J1-2 = 3.7 Hz, 1, H1), 6.07 (t, J4-3/5
=
10.4 Hz, 1, H4), 7.44–8.20 (m, 10, Ar); 13C NMR d
27.17 and 27.39 (CMe2), 70.71 (d, J4-F = 8.5 Hz, C4),
3
3
4
C4), 77.38 (d, J3-F = 1.4 Hz, C3), 77.52 (C2), 104.53
(C1), 113.47 (CMe2), 121.24 (d, J5-F = 9.5 Hz, C5),
2
77.17 (C3), 77.86 (C2), 104.82 (C1), 114.37 (CMe2),
116.23 (d, J5-F = 16.2 Hz, C5), 128.95 (Ph), 129.22
2
128.75 (Bz), 129.88 (Bz), 130.34 (Bz), 133.73 (Bz),
166.35 (Bz), 180.03 (d, J6-F = 254.3 Hz, C6).
2
(Bz), 129.46 (Bz), 129.80 (Ph), 130.02 (Ph), 133.61
=
296.3 Hz, C6), 166.30 (Bz).
1
(Bz), 134.00 (Ph), 135.19 (Ph), 156.62 (d, J6-F
5.11. (E/Z)-3-O-Benzoyl-5,6-dideoxy-6-fluoro-1,2-O-isopro-
pylidene-6-tributylstannyl-a-D-ribo-hex-5-enofuranose (24)
5.9. (E)-3-O-Benzoyl-5,6-dideoxy-1,2-O-isopropylidene-
6-phenylsulfonyl-a-D-ribo-hex-5-enofuranose (22)
Treatment of 21 (300 mg, 0.70 mmol; E/Z, 3:2) with
Bu3SnH (407 mg, 0.38 mL, 1.4 mmol) and AIBN
(86 mg, 0.53 mmol) as described for 23 gave 24
(397 mg, 95%; E/Z, 3:2): MS m/z 599 (89, MH+,
120Sn), 597 (63, MH+, 118Sn), 595 (33, MH+, 116Sn),
541 (100, M-57, 120Sn), 539 (78, M-57, 118Sn), 537 (42,
M-57, 116Sn); 19F NMR d ꢁ87.58 (d, JF-H5 = 33.1 Hz,
84% of 0.40F, Z), ꢁ87.58 (ddd, JF-Sn = 226.7 Hz,
JF-H5 = 32.8 Hz, JF-H4 = 4.1 Hz, 16% of 0.40F), ꢁ94.80
(d, JF-H5 = 51.1 Hz, 84% of 0.60F, E), ꢁ94.80 (ddd,
JF-Sn = 213.9 Hz, JF-H5 = 50.8 Hz, JF-1H4 = 4.5 Hz, 16%
of 0.60F, E). Compound (E)-24 had: H NMR d 0.70–
1.70 (m, 27, 3· Bu), 1.24 and 1.26 (2· s, 2 · 3, 2·
CH3), 4.62 (dd, J3-4 = 9.3 Hz, J3-2 = 4.7 Hz, 1, H3),
Treatment of 9 (150 mg, 0.50 mmol) with diethyl (phenyl-
sulfonyl)methylphosphonate20 (146 mg, 0.50 mmol) and
LHMDS (0.50 mL, 84 mg, 0.50 mmol) as described for
20 gave 22 (166 mg, 82%): H NMR d 1.33 and 1.55 (2·
s, 2 · 3, 2· CH3), 4.76 (dd, J3-4 = 9.5 Hz, J3-2 = 4.6 Hz, 1,
H3), 4.92 (ddd, J4-5 = 3.7 Hz, J4-6 = 1.7 Hz, J4-3 = 9.5 Hz,
1
1, H4), 5.01 (‘t’, J2-3/1 = 4.2 Hz, 1, H2), 5.90 (d, J1-2
=
3.7 Hz, 1, H1), 6.79 (dd, J6-4 = 1.8 Hz, J6-5 = 15.0 Hz, 1,
H6), 7.09 (dd, J5-6 = 15.0 Hz, J5-4 = 3.8 Hz, 1, H5),
7.50–8.05 (m, 10, Ar); MS m/z 431 (100, MH+).
5.10. (E/Z)-3-O-Benzoyl-5,6-dideoxy-6-fluoro-1,2-O-iso-
propylidene-6-tributylstannyl-a-D-xylo-hex-5-enofura-
nose (23)
4.86–4.87 (m, 1, H2), 4.90 (dd, J5-F = 51.0 Hz, J5-4
=
8.4 Hz, 1, H5), 5.28 (‘t’, J4-3/5 = 8.9 Hz, 1, H4), 5.79 (d,
J1-2 = 3.9 Hz, 1, H1), 7.57–8.06 (m, 5, Ar); 13C NMR
d 11.15 (Bu), 14.03 (Bu), 29.96 (Bu), 27.48 and 27.59
Bu3SnH (407 mg, 0.38 mL, 1.4 mmol) was added drop-
wise to a degassed solution of 20 (300 mg, 0.70 mmol;
E/Z, 7:3) in anhydrous toluene (5 mL) in a flame-dried
flask under N2 at ambient temperature. After an addi-
tional 10 min of degassing with N2, AIBN (86 mg,
0.53 mmol) was added and the reaction mixture was re-
fluxed at 110 ꢁC with stirring for 5 h. The volatiles were
evaporated and the residue was partitioned between
EtOAc (50 mL) and NaHCO3/H2O (30 mL). The organ-
ic layer was washed with NaCl/H2O (30 mL), dried
(Na2SO4), and evaporated. Column chromatography
(hexanes ! 10% EtOAc/hexanes) gave 23 (794 mg,
95%; E/Z, 7:3) as an inseparable mixture: MS m/z 599
(89, MH+, 120Sn), 597 (63, MH+, 118Sn), 595 (33,
MH+, 116Sn), 541 (100, M-57, 120Sn), 539 (78, M-57,
118Sn), 537 (42, M-57, 116Sn); 19F NMR d ꢁ87.67 (d,
3
(CMe2), 29.23 (Bu), 70.55 (d, J4-F = 18.1 Hz, C4),
77.36 (C3), 77.48 (C2), 104.50 (C1), 113.54 (CMe2),
120.59 (d, J5-F = 3.8 Hz, C5), 128.77 (Bz), 129.81 (Bz),
2
1
130.38 (Bz), 133.69 (Bz), 166.44 (Bz), 176.10 (d, J6-
1
F = 260.0 Hz, C6). Compound (Z)-24 had: H NMR d
0.70–1.70 (m, 27, 3· Bu), 1.28 and 1.30 (2· s, 2 · 3, 2·
CH3), 4.47 (‘t’, J4-3/5 = 9.3 Hz, 1, H4), 4.71 (dd, J3-4
=
9.0 Hz, J3-2 = 4.8 Hz, 1, H3), 4.85–4.86 (m, 1, H2),
5.81 (d, J1-2 = 3.8 Hz, 1, H1), 5.83 (dd, J5ꢁF = 33.7 Hz,
J5-4 = 9.5 Hz, 1, H5), 7.57–8.06 (m, 5, Ar); 13C NMR
d 11.15 (Bu), 14.03 (Bu), 29.96 (Bu), 27.48 and 27.59
3
(CMe2), 29.23 (Bu), 74.72 (d, J4-F = 22.0 Hz, C4),
77.16 (C3), 77.67 (C2), 104.28 (C1), 113.13 (CMe2),
121.18 (d, J5-F = 9.9 Hz, C5), 128.77 (Bz), 129.76 (Bz),
2
1
130.28 (Bz), 130.80 (Bz), 166.24 (Bz), 177.00 (d, J6-F
255.0 Hz, C6).
=
JF-H5 = 34.3 Hz, 84% of 0.30F, Z), ꢁ87.67 (dd, JF-Sn
=
229.5 Hz, JF-H5 = 34.8 Hz, 16% of 0.30F, Z), ꢁ92.73 (d,
JF-H5 = 52.7 Hz, 84% of 0.70F, E), ꢁ92.73 (ddd, JF-Sn
=
5.12. (E)-3-O-Benzoyl-5,6-dideoxy-1,2-O-isopropylidene-
6-tributylstannyl-a-D-ribo-hex-5-enofuranose (25)
213.1 Hz, JF-H5 = 52.7 Hz, JF-H4 = 4.9 Hz, 16% of
0.70F, E). Compound (E)-23 had: 1H NMR d 0.90–
1.60 (m, 27, 3· Bu), 1.34 and 1.36 (2· s, 2 · 3, 2·
CH3), 4.71 (d, J2-1 = 3.8 Hz, 1, H2), 5.10 (dd, J5-F
= 52.6 Hz, J5-4 = 7.4 Hz, 1, H5), 5.32 (d, J3-4 = 3.0 Hz,
1, H3), 5.47–5.49 (m, 1, H4), 6.02 (d, J1-2 = 3.8 Hz, 1,
H1), 7.47–8.06 (m, 5, Ar); 13C NMR d 10.33 (Bu),
11.15 (Bu), 17.90 (Bu), 27.42 and 27.54 (CMe2), 28.24
(Bu), 70.56 (d, ꢁ3J4-F = 17.6 Hz, C4), 77.21 (C3),
Treatment of 22 (E; 300 mg, 0.70 mmol) with Bu3SnH
(407 mg, 0.38 mL, 1.4 mmol) and AIBN (86 mg,
0.53 mmol) as described for 23 gave 25 (385 mg, 95%):
1H NMR d 1.51–1.90 (m, 33, 3· Bu and 2· CH3), 4.66
(dd, J4-5 = 6.5 Hz, J4-3 = 8.4 Hz, 1, H4), 4.77 (dd,
J3-4 = 9.2 Hz, J3-2 = 4.7 Hz, 1, H3), 4.97 (t, J2-1/3
=
77.52 (C2), 104.31 (C1), 113.07 (CMe2), 120.53 (d, 2J5-F
=
3.6 Hz, 1, H2), 5.93 (d, J1-2 = 3.8 Hz, 1, H1), 6.10
(dd, J5-6 = 19.1 Hz, J5-4 = 6.5 Hz 1, H5), 6.50 (dd,
J6-5 = 19.1 Hz, J6-4 = 0.9 Hz, 1, H6), 7.57–8.06 (m, 5,
Ar); MS m/z 581 (89, MH+, 120Sn), 579 (63, MH+,
118Sn), 577 (33, MH+, 116Sn), 523 (100, M-57, 120Sn),
521 (78, M-57, 118Sn), 519 (42, M-57, 116Sn).
3.9 Hz, C5), 128.75 (Bz), 129.83 (Bz), 130.25 (Bz), 133.61
(Bz), 166.16 (Bz), 177.14 (d, 2J6-F = 262.0 Hz, C6). Com-
pound (Z)-23 had: 1H NMR d 0.90–1.60 (m, 27, 3· Bu),
1.38 and 1.69 (2· s, 2 · 3, 2· CH3), 4.69 (d, J1-2
=
3.9 Hz, 1, H2), 4.75 (d, J3-4 = 7.9 Hz, 1, H3), 5.47-5.49

S. F. Wnuk et al. / Bioorg. Med. Chem. 16 (2008) 5090–5102
5097
5.13. (E/Z)-3-O-Benzoyl-5,6-dideoxy-6-fluoro-6-iodo-1,2-
O-isopropylidene-a-D-xylo-hex-5-enofuranose (26)
16.2 Hz, C5), 128.91 (Bz), 129.49 (Bz), 130.37 (Bz),
133.95 (Bz), 166.21 (Bz).
A solution of NIS (50 mg, 0.23 mmol) in anhydrous
CH2Cl2 (3 mL) was added dropwise to a stirred solution
of 23(90 mg, 0.15 mmol; E/Z, 7:3) in CH2Cl2 (5 mL) under
N2 at ꢁ20 ꢁC. After 1 h, CHCl3 (30 mL) and diluted NaH-
SO3/H2O (10 mL) were added. The separatedorganic layer
was washed with NaHCO3/H2O (10 mL), NaCl/H2O
(10 mL), dried (Na2SO4), and evaporated. Column chro-
matography (hexanes ! 30% EtOAc/hexanes) gave 26
(155 mg, 83%; E/Z, 8:2) as an inseparable mixture: MS
5.15. (E)-3-O-Benzoyl-5,6-dideoxy-6-iodo-1,2-O -isopro-
pylidene-a-D-ribo-hex-5-enofuranose (28)
Treatment of 25 (150 mg, 0.25 mmol) with NIS (85 mg,
0.38 mmol) as described for 26 gave 28 (93 mg, 87%): 1H
NMR d 1.35 and 1.62 (2· s, 2 · 3, 2· CH3), 4.67 (dd, J2-3
=
9.2 Hz, J2-1 = 4.6 Hz, 1, H2), 4.77 (dd, J3-4 = 3.4 Hz,
J3-2 = 9.2 Hz, 1, H3), 4.97 (t, J4-3/5 = 4.2 Hz, 1, H4),
5.91 (d, J1-2 = 3.8 Hz, 1, H1), 6.61–6.70 (m, 2, H5/6),
7.49–8.08 (m, 5, Ar); 13C NMR d 26.95 and 26.96
(CMe2), 76.37 (C4), 77.59 (C3), 79.61 (C2), 81.32 (C6),
104.43 (C1), 113.68 (CMe2), 128.89 (Bz), 129.60 (Bz),
130.94 (Bz), 133.89 (Bz), 141.58 (C5), 166.08 (Bz); MS
m/z 417 (100, MH+).
m/z 435 (100, MH+); 19F NMR d ꢁ56.54 (d, JF-H5
=
15.8 Hz, 0.20F, Z), ꢁ60.98 (d, JF-H5 = 33.1 Hz, 0.80F,
1
E). Compound (E)-26 had: H NMR d 1.35 and 1.38
(2· s, 2 · 3, 2· CH3), 4.69 (d, J2-1 = 3.8 Hz, 1, H2), 5.28
(ddd, J4-5 = 9.8 Hz, J4-3 = 2.9 Hz, J4-6 = 1.6 Hz, 1, H4),
5.45 (d, J3-4 = 3.0 Hz, 1, H3), 5.58 (dd, J5-F = 33.0 Hz,
J5-4 = 8.7 Hz, 1, H5), 5.99 (d, J1-2 = 3.7 Hz, 1, H1),
7.47–8.06 (m, 5, Ar); 13C NMR d 26.60 and 27.12
5.16. Ethyl 3-O-benzoyl-5,6,7,8,9-pentadeoxy-6-fluoro-1,
2-O-isopropylidene-a-D-xylo-dec-5-(E/Z)-enofuranuro-
nate (29)
3
(CMe2), 74.04 (d, J4-F = 4.4 Hz, C4), 77.88 (C3), 83.73
1
(C2), 104.74 (C1), 107.89 (d, J6-F = 338.7 Hz, C6),
2
112.85 (CMe2), 116.80 (d, J5-F = 5.4 Hz, C5), 129.04
(Bz), 129.50 (Bz), 130.18 (Bz), 134.09 (Bz), 165.54 (Bz).
Pd[P(Ph)3]4 (5 mg, 0.004 mmol) was added to a stirred
solution of 26 (30 mg, 0.07 mmol; E/Z, 4:1) in anhy-
drous benzene (3 mL) under N2 at ambient temperature.
After 2 min, 4-ethoxy-4-oxobutylzinc bromide (0.5 M/
THF; 0.28 mL, 65 mg, 0.14 mmol) was added and the
resulting mixture was heated at 55 ꢁC for 5 h. EtOAc
(30 mL) and NaHCO3/H2O (10 mL) were added and
the separated organic layer was washed with H2O
(10 mL), NaCl/H2O (10 mL), dried (Na2SO4), and then
was evaporated. Column chromatography (10 ! 30%
EtOAc/hexanes) gave (Z)-29 (18 mg, 61%, 76% based
on the conversion of the E-isomer), (E)-29 (2 mg, 7%,
35% based on the conversion of the Z-isomer) and more
polar byproduct tentatively assigned as 3-O-debenzoy-
1
Compound (Z)-26 had: H NMR d 1.35 and 1.68 (2· s,
2 · 3, 2· CH3), 4.71 (d, J1-2 = 3.7 Hz, 1, H2), 4.84 (dd,
J4-5 = 8.7 Hz, J4-3 = 2.7 Hz, 1, H4), 5.48 (d, J3-4
=
3.0 Hz, 1, H3), 5.77 (dd, J5-F = 15.4 Hz, J5-4 = 8.8 Hz, 1,
H5), 6.03 (d, J1-2 = 3.7 Hz, 1, H1), 7.47–8.06 (m, 5, Ar);
13C NMR d 26.78 and 27.27 (CMe2), 77.88 (C3), 80.06
3
(d, J4-F = 8.2 Hz, C4), 83.84 (C2), 105.04 (C1), 112.94
(d, 2J5-F = 16.9 Hz, C5), 112.95 (CMe2), 114.78 (d, 1J6-F
=
332.0 Hz, C6), 129.04 (Bz), 129.40 (Bz), 130.18 (Bz),
134.14 (Bz), 165.54 (Bz).
5.14. (E/Z)-3-O-Benzoyl-5,6-dideoxy-6-fluoro-6-iodo-1,2-
O-isopropylidene-a-D-ribo-hex-5-enofuranose (27)
lated-(Z)-26 [ꢀ5%, TLC; 19F NMR d ꢁ57.21 (JF-H5
=
1
16.4 Hz)]. Compound (Z)-29 had: H NMR d 1.22 (t,
J = 7.1 Hz, 3, CH3), 1.36 and 1.61 (2· s, 2 · 3, 2·
Treatment of 24 (250 mg, 0.42 mmol; E/Z, 3:2) with NIS
(142 mg, 0.63 mmol) as described for 26 gave 27
(155 mg, 85%; E/Z, 3:2) as an inseparable mixture:
HRMS (AP-FAB) m/z: calcd for C16H16FIO5Li
(MLi+) 441.0181; found: 441.0192. 19F NMR d ꢁ56.42
CH3), 1.81 (‘quint’, J8-7/7 /9/9 = 7.4 Hz, 2, H8/80), 2.26
0
0
0
0
(dt, J7-F = 18.1 Hz, J7-8/8 = 7.4 Hz, 2, H7/7 ), 2.30 (t,
0
J9-8/8 = 7.4 Hz, 2, H9/90), 4.09 (q, J = 7.1 Hz, 2, CH2),
4.69 (d, J2-1 = 3.7 Hz, 1, H2), 4.84 (dd, J5-F = 35.8 Hz,
J5-4 = 8.4 Hz, H5), 5.33 (dd, J4-5 = 8.5 Hz, J4-3 = 2.8 Hz,
1, H4), 5.45 (d, J3-4 = 2.8 Hz, 1, H3), 6.00 (d, J1-2 =
(d, JF-H5 = 15.1 Hz, 0.40F, Z), ꢁ63.30 (d, JF-H5
=
1
33.5 Hz, 0.60F, E). Compound (E)-27 had: H NMR d
1.36 and 1.60 (2· s, 2 · 3, 2· CH3), 4.75 (dd, J3-4
9.2 Hz, J3-2 = 4.7 Hz, 1, H3), 4.98 (t, J2-1/3 = 4.5 Hz, 1,
H2), 5.16 (t, J4-3/5 = 9.0 Hz, 1, H4), 5.49 (dd, J5-F
=
3.7 Hz, 1, H1), 7.48–8.04, (m, 5, Ar); 13C NMR d
14.59 (CH3), 21.49 (C8), 26.64 and 27.11 (CMe2),
31.61 (d, J7-F = 25.4 Hz, C7), 33.34 (C9), 60.75 (CH2),
2
=
3
32.7 Hz, J5-4 = 8.7 Hz, 1, H5), 5.89 (d, J1-2 = 3.8 Hz, 1,
H1), 7.50–8.10 (m, 5, Ar); 13C NMR d 26.96 and 27.11
73.37 (d, J4-F = 6.6 Hz, C4), 77.63 (C2), 78.33 (C3),
100.03 (d, J5-F = 10.9 Hz, C5), 104.78 (C1), 112.60
(CMe2), 128.97 (Bz), 129.70 (Bz), 130.16 (Bz), 133.93
2
3
(CMe2), 72.45 (d, J4-F = 4.3 Hz, C4), 76.74 (d, J3-F
4
=
2.1 Hz, C3), 77.32 (C2), 104.39 (C1), 113.78 (CMe2),
1
(Bz), 162.94 (d, J6-F = 260.7 Hz, C6), 165.61 (Bz),
1
2
114.96 (d, J6-F = 331.5 Hz, C6), 119.90 (d, J5-F
=
173.32 (C10); 19F NMR d ꢁ99.93 (dt, JF-H5 = 35.8 Hz,
J = 18.0 Hz); HRMS (AP-FAB+) m/z calcd for
C22H27FO7Li (MLi+) 429.1910; found: 429.1900. Com-
5.5 Hz, C5), 128.92 (Bz), 129.46 (Bz), 130.50 (Bz),
133.94 (Bz), 166.21 (Bz). Compound (Z)-27 had: 1H
NMR d 1.38 and 1.64 (2· s, 2 · 3, 2· CH3), 4.72–4.75
(m, 1, H4), 4.84 (dd, J3-4 = 9.2 Hz, J3-2 = 4.6 Hz, 1,
1
pound (E)-29 had: H NMR d 1.28 (t, J = 7.1 Hz, 3,
CH3), 1.35 and 1.61 (2· s, 2 · 3, 2· CH3), 1.85–1.95
(m, 2, H8/80), 2.38 (t, J9-8/8 = 7.2 Hz, 2, H9/90), 2.39–
0
H3), 4.98 (t, J2-3/1 = 4.5 Hz, 1, H2), 5.68 (dd, J5-F
15.3 Hz, J5-4 = 8.9 Hz, 1, H5), 5.91 (d, J1-2 = 3.8 Hz, 1,
=
2.50 (m, 2, H7/70), 4.15 (q, J = 7.1 Hz, 2, CH2), 4.69
H1), 7.50–8.14 (m, 5, Ar); 13C NMR d 26.96 and 27.11
(CMe2), 76.85 (d, J3-F = 2.1 Hz, C3), 77.63 (C2), 78.13
(d, J2-1 = 3.8 Hz, 1, H2), 4.93 (‘dt’, J4-5 = 9.3 Hz, J4-F/3
2.5 Hz, 1, H4), 5.30 (dd, J5-F = 18.6 Hz, J5-4 = 9.4 Hz,
H5), 5.38 (d, J3-4 = 2.9 Hz, 1, H3), 6.00 (d, J1-2
3.8 Hz, 1, H1), 7.48–8.04 (m, 5, Ar); 19F NMR d
=
4
3
(d, J4-F = 8.3 Hz, C4), 104.54 (C1), 108.75 (d, J6-F
1
=
=
=
2
339.4 Hz, C6), 113.90 (CMe2), 115.77 (d, J5-F

5098
S. F. Wnuk et al. / Bioorg. Med. Chem. 16 (2008) 5090–5102
ꢁ94.53 (‘q’, J = 22.1 Hz). HRMS (AP-FAB+) m/z calcd
was added at 0 ꢁC (ice bath). The resulting mixture
was stirred for 48 h (0 ꢁC ! ambient temperature).
The volatiles were evaporated and the residue was col-
umn chromatographed (15 ! 50% EtOAc/hexanes) to
for C22H27FO7Li (MLi+) 429.1910; found: 429.1903.
5.17. Ethyl 3-O-benzoyl-5,6,7,8,9-pentadeoxy-6-fluoro-1,2-
O-isopropylidene-a-D-ribo-dec-5(E/Z)-enofuranuronate (30)
1
give 31 (14 mg, 74%): H NMR d 1.35 and 1.55 (2· s,
0
0
2 · 3, 2· CH3), 1.90 (quint, J8-7/7 /9/9 = 7.2 Hz, 2, H8/
80), 2.23–2.40 (m, 2, H7/70), 2.41 (t, J9-8/8 = 7.2 Hz, 2,
0
Treatment of 27 (42 mg, 0.097 mmol; E/Z, 3:2) with
Pd[P(Ph)3]4 (22 mg, 0.01 mmol) and 4-ethoxy-4-oxobu-
tylzinc bromide (0.5 M/THF; 0.30 mmol, 0.60 mL) as de-
scribed for 29 followed by column chromatography
(10 ! 40% EtOAc/hexanes) gave (Z)-30 (22 mg, 54%;
90% based on the conversion of E-isomer), (E)-30
(5 mg, 12%; 30% based on the conversion of the Z-iso-
mer), and more polar 3-O-debenzoylated-(Z)-27 (3 mg,
10%). Compound (Z)-30 had: 1H NMR d 1.24 (t,
J = 7.1 Hz, 3, CH3), 1.37 and 1.60 (2· s, 2 · 3, 2· CH3),
H9/90), 3.70 (s, 3, CH3), 4.17 (d, J3-4 = 2.6 Hz, 1, H3);
4.59 (d, J2-1 = 3.7 Hz, 1, H2), 4.84 (dd, J5-F = 37.6 Hz,
J5-4 = 7.7 Hz, 1, H5), 5.08 (‘dm’, J4-5 = 7.6 Hz, 1, H4),
5.95 (d, J1-2 = 3.7 Hz, 1, H1); 13C NMR d 21.40 (C8),
2
26.59 and 27.12 (CMe2), 31.65 (d, J7-F = 26.6 Hz, C7),
3
33.29 (C9), 52.08 (CH3), 75.29 (d, J4-F = 4.9 Hz, C4),
4
76.74 (d, J3-F = 1.0 Hz, C3), 85.51 (C2), 101.15 (d,
2J5-F = 11.0 Hz, C5), 104.74 (C1), 112.08 (CMe2),
1
161.86 (d, J6-F = 261.8 Hz, C6), 174.02 (C10); 19F
1.84 (‘quint’, J8-7/7 /9/9 = 7.4 Hz, 2, H8/80), 2.25 (dt, J7-F
=
=
NMR
d
ꢁ100.23 (dt, JF-H5 = 38.0 Hz, JF-H7
=
0
0
17.6 Hz, J7-8/8 = 7.5 Hz, 2, H7/70), 2.32 (t, J9-8/8
7.4 Hz, 2, H9/90), 4.09 (q, J = 7.1 Hz, 2, CH2), 4.72 (dd,
18.0 Hz); MS m/z 305 (100, MH+).
0
0
J3-4 = 9.2 Hz, J3-2 = 4.7 Hz, 1, H3), 4.75 (dd, J5-F
=
5.19. Methyl 5,6,7,8,9-Pentadeoxy-6-fluoro-1,2-O-iso-
propylidene-a-D-ribo-dec-5(Z)-enofuranuronate (32)
35.0 Hz, J5-4 = 8.9 Hz, 1, H5), 4.95 (t, J2-1/3 = 4.3 Hz, 1,
H2), 5.19 (t, J4-3/5 = 9.1 Hz, 1, H4), 5.89 (d, J1-2
= 3.8 Hz, 1, H1), 7.48-8.09 (m, 5, Ar); 13C NMR d 14.60
(CH3), 21.45 (C8), 26.95 and 27.00 (CMe2), 31.63 (d,
2J7- F = 26.5 Hz, C7), 33.32 (C9), 60.79 (CH2), 71.39 (d,
3J4-F = 6.3 Hz, C4), 77.54 (C2), 73.63 (C3), 103.40 (d,
2J5-F = 11.8 Hz, C5), 104.39 (C1), 113.52 (CMe2),
128.84 (Bz), 129.71 (Bz), 130.33 (Bz), 133.77 (Bz),
Saturated NH3/MeOH (3 mL) was added to a solution
of (Z)-30 (26 mg, 0.062 mmol) in MeOH (3 mL) and
the mixture was stirred at 0 ꢁC for 48 h to ambient tem-
perature. The volatiles were evaporated and the residue
was column chromatographed (15 ! 60% EtOAc/hex-
1
1
anes) to give 32 (13 mg, 69%): H NMR d 1.39 and
164.13 (d, J6-F = 272.7 Hz, C6), 165.33 (Bz), 173.30
(C10); 19F NMR d ꢁ102.14 (dt, JF-H5 = 34.1 Hz, JF-H7/7
0
0
0
1.62 (2· s, 2 · 3, 2· CH3), 1.90 (quint, J8-7/7 /9/9
=
=
17.6 Hz). HRMS (AP-ESI) m/z calcd for C22H28FO7
7.3 Hz, 2, H8/80), 2.31 (dt, J7-F = 17.6 Hz, J7-8/8
=
0
(MH+) 423.1814; found 423.1815. Compound (E)-30
7.4 Hz, 2, H7/70), 2.40 (t, J9-8/8 = 6.9 Hz, 2, H9/90),
0
1
3.75 (s, 3, CH3), 4.56–4.72 (m, 4, H2/3/4/5), 5.82 (d, J1-2
=
had: H NMR d 1.26 (t, J = 7.1 Hz, 3, CH3), 1.36 and
3.9 Hz, 1, H1); 13C NMR d 21.44 (C8), 26.81 and 26.94
(CMe2), 31.74 (d, 2J7-F = 26.1 Hz, C7), 33.20 (C9), 52.07
(CH3), 73.94 (d, J4-F = 5.1 Hz, C4), 76.80 (C2), 78.62
0
0
=
1.61 (2· s, 2 · 3, 2· CH3), 1.90 (‘quint’, J8-7/7 /9/9
6.9 Hz, 2, H8/80), 2.38 (t, J9-8/8 = 6.9 Hz, 2, H9/90), 2.50
0
3
(dt, J7-F = 23.0 Hz, J7-8/8 = 7.3 Hz, 2, H7/70), 4.14 (q,
0
2
(C3), 103.63 (d, J5-F = 11.6 Hz, C5), 104.00 (C1),
113.07 (CMe2), 163.91 (d, J6-F = 262.70 Hz, C6),
J = 7.1 Hz, 2, CH2), 4.75-4.80 (m, 2, H3/4), 4.95 (t, J2-1/3
4.0 Hz, 1, H2), 5.17 (dd, J5-F = 19.2 Hz, J5-4
=
=
1
173.90 (C10); 19F NMR d ꢁ100.23 (dt, JF-H5
=
37.1 Hz, JF-H7/7 = 18.1 Hz). HRMS (AP-ESI) m/z calcd
8.7 Hz, 1, H5), 5.88 (d, J1-2 = 3.8 Hz, 1, H1), 7.48–8.09
(m, 5, Ar); 13C NMR d 14.63 (CH3), 21.99 (C8), 26.92
and 27.00 (CMe2), 28.49 (d, J7-F = 27.0 Hz, C7), 33.67
0
for C14H22FO6 (MH+) 305.1395; found: 305.1396.
2
3
(C9), 60.78 (CH2), 73.65 (d, J4-F = 14.7 Hz, C4), 77.50
2
(C2), 77.61 (C3), 104.58 (d, J5-F = 25.8 Hz, C5), 104.37
(C1), 113.39 (CMe2), 128.88 (Bz), 129.64 (Bz), 130.25
(Bz), 133.83 (Bz), 165.15 (d, J6-F = 256.5 Hz, C6),
5.20. Methyl 5,6,7,8,9-pentadeoxy-6-fluoro-a/b-D-xylo-
dec-5(Z)-enofuranuronate (33)
1
165.99 (Bz), 173.21 (C10); 19F NMR d ꢁ94.73 (‘q’,
0
JF-H5/7/7 = 22.8 Hz). HRMS (AP-ESI) m/z calcd for
A solution of 31 (17 mg, 0.056 mmol) in TFA/H2O
(9:1, 3 mL) was stirred for 45 min at 0 ꢁC and was
evaporated and coevaporated [toluene (3·), CH3CN
(2·)]. The residue was dissolved in H2O and the aque-
ous layer was extracted with ether (2·). The water
layer was evaporated to give 33 (9 mg, 61%; a/b,
C22H28FO7 (MH+) 423.1814; found: 423.1819. The 3-O-
debenzoylated-(Z)-27 had: 1H NMR d 1.27 and 1.58
(2· s, 2 · 3, 2· CH3), 3.82–3.84 (m, 1, H3), 4.18 (t, J4-3/5
8.8 Hz, 1, H4), 4.60–4.62 (m, 1, H2), 5.61 (dd, J5-F
=
=
15.1 Hz, J5-4 = 8.9 Hz, 1, H5), 5.83 (d, J1-2 = 3.7 Hz, 1,
H1); 13C NMR d 26.99 and 27.07 (CMe2), 76.63 (C3),
78.56 (C2), 80.47 (d, J4-F = 8.0 Hz, C4), 104.16 (C1),
1
1:1): H NMR (MeOH-d4)d 1.81–1.94 (m, 2, H8/80),
3
2.27–2.38 (m, 2, H7/70), 2.38–2.45 (m, 2, H9/90), 3.67
(m, 3, CH3), 3.92 (dd, J3-4 = 3.8 Hz, J3-2 = 1.8 Hz,
0.5, H3), 3.97 (dd, J3-4 = 3.9 Hz, J3-2 = 2.6 Hz, 0.5,
H3), 4.00–4.04 (m, 1, H2), 4.91 (d, J4-5 = 8.9 Hz, 0.5,
H4), 4.99 (d, J4-5 = 9.1 Hz, 0.5, H4), 5.04–5.12 (m, 1,
H5), 5.08 (s, 0.5, H1b), 5.37 (d, J1-2 = 4.0 Hz, 0.5,
1
115.85 (d, J6-F = 344.1 Hz, C6), 113.70 (CMe2), 115.85
2
(d, J5-F = 15.7 Hz, C5); 19F NMR d ꢁ56.50 (d, JF-H5
=
15.1 Hz); MS m/z 331 (100, MH+).
5.18. Methyl 5,6,7,8,9-pentadeoxy-6-fluoro-1,2-O-iso-
propylidene-a-D-xylo-dec-5(Z)-enofuranuronate (31)
H1a); 19F NMR d ꢁ106.29 (dt, JF- H5 = 37.2 Hz, JF-H7
17.6 Hz, 0.5F), ꢁ106.87 (dt, JF-H5 = 37.8 Hz, JF-H7
C11H18FO6 (MH+) 265.1082; found: 265.1088.
=
=
18.2 Hz, 0.5F). HRMS (AP-ESI) m/z calcd for
Compound (Z)-29 (26 mg, 0.062 mmol) was dissolved in
MeOH (6 mL) and the saturated NH3/MeOH (3 mL)

S. F. Wnuk et al. / Bioorg. Med. Chem. 16 (2008) 5090–5102
5099
5.21. Methyl 5,6,7,8,9-pentadeoxy-6-fluoro-a/b-D-ribo-
dec-5(Z)-enofuranuronate (34)
JF-H5 = 52.7 Hz, 84% of 0.50F, E), ꢁ96.75 (ddd, JF-Sn
=
221.1 Hz, JF-H5 = 52.7 Hz, JF-H4 = 4.9 Hz, 16% of
0.50F, E); MS m/z 479 (100, MH+, 120Sn), 477 (73,
MH+, 118Sn), 475 (48, MH+, 116Sn). Compound (E)-38
A solution of 32 (12 mg, 0.04 mmol) in TFA/H2O (9:1,
3 mL) was stirred for 30 min at 0 ꢁC and was evaporated
and coevaporated [toluene (3·)]. The residue was dis-
solved in H2O and the aqueous layer was extracted with
ether (2·). The water layer was evaporated to give 34
1
had: H NMR d 0.98–1.70 (m, 34, 3· Bu/2· CH3/H3),
0
0
2.26 (dd, J3 -4 = 4.3 Hz, J3 -3 = 13.4 Hz, 1, H3), 4.45–
4.55 (m, 1, H4), 4.75 (t, J2-1/3 = 4.2 Hz, 1, H2), 4.96
(dd, J5-F = 52.9 Hz, J5-4 = 7.5 Hz, 1, H5), 5.83 (d, J1-2
=
1
(8 mg, 76%; a/b, 3:7): H NMR (D2O) d 1.72–1.76 (m,
3.8 Hz, 1, H1); 13C NMR d 10.31 (Bu), 11.00 (Bu),
13.97 (Bu), 27.04 and 27.08 (CMe2), 27.50 (Bu), 40.19
(d, J3-F = 1.6 Hz, C3), 71.25 (d, J4-F = 17.3 Hz, C4),
2, H8/80), 2.20 (dt, J7-F = 18.1 Hz, J7-8/8 = 8.4 Hz, 2,
0
2
3
H7/70), 2.31–2.38 (m, 2, H9/90), 3.59 (d, J2-3 = 2.4 Hz,
0.7, H2), 3.82–3.85 (m, 0.3, H2), 3.90–3.93 (s, 0.7, H3),
3.90–4.06 (m, 4.3, H3/H4/CH3), 4.64-4.77 (m, 1, H5),
5.12 (s, 0.7, H1), 5.28 (d, J1-2 = 3.7 Hz, 0.3, H1); 19F
0
81.06 (C2), 105.47 (C1), 111.41 (CMe2), 123.42 (d, 2J5-F
=
3.7 Hz, C5), 174.29 (d, J5-F = 323.5 Hz, C6). Com-
1
pound (Z)-38 had: 1H NMR d 0.98–1.70 (m, 34, 3·
0
0
NMR
d
ꢁ104.06 (dt, JF-H5 = 36.4 Hz, JF-H7/7
=
=
Bu/2· CH3/H3), 2.11 (dd, J3 -4 = 4.3 Hz, J3 -3 = 13.4 Hz,
17.8 Hz, 0.3, a); ꢁ105.04 (dt, JF-H5 = 35.8 Hz, JF-H7/7
1, H30), 4.73 (t, J2-1/3 = 4.2 Hz, 1, H2), 5.21 (ddd, J4-5
=
7.5 Hz, J4-3 = 4.4 Hz, J4-3 = 15.2 Hz, 1, H4), 5.81 (d,
J1-2 = 3.7 Hz, 1, H1), 5.84 (dd, J5-F = 34.2 Hz, J5-4 =
0
0
18.8 Hz, 0.7F, b). HRMS (AP-ESI) m/z calcd for
C11H18FO6 (MH+) 265.1082; found: 265.1090.
9.2 Hz, 1, H5); 13C NMR d 10.31 (Bu), 11.00 (Bu),
13.97 (Bu), 27.04 and 27.08 (CMe2), 27.50 (Bu), 41.00
5.22. (E/Z)-3,5,6-Trideoxy-6-fluoro-1,2-O-isopropylidene-
6-phenylsulfonyl-a-D-erythro-hex-5-enofuranose (35)
2
3
(d, J3-F = 1.8 Hz, C3), 74.73 (d, J4-F = 21.7 Hz, C4),
80.83 (C2), 105.69 (C1), 111.17 (CMe2), 123.26 (d,
1
Step (a). Treatment of diacetone 3-deoxyglucose22
(204 mg, 0.83 mmol) with H5IO6 (228 mg, 1.00 mmol)
as described for 10 (Step (a), except no aqueous workup
was performed) gave 3-deoxy-1,2-O-isopropylidene-a-D-
2J5-F = 8.1 Hz, C5), 177.16 (d, J6-F = 316.5 Hz, C6).
5.24. (E/Z)-5,6-Dideoxy-6-fluoro-1,2-O-isopropylidene-a-
D-xylo-hex-5-enofuranose (39)
1
erythro-pentdialdo-1,4-furanose [ꢀ85% pure; H NMR
d 9.75 (d, J5-4 = 4.8 Hz, H5)] was directly used in the next
step. Step (b). Treatment of the crude aldehyde with
Step (a). Compound 23 (200 mg, 0.34 mmol; E/Z, 7:3)
was dissolved in saturated NH3/MeOH (20 mL) and the
resulting solution was stirred overnight at ambient tem-
perature. The volatiles were evaporated to give 36 in
quantitative yield of sufficient purity for use in the subse-
quent reaction. Step (b). Compound 36 (crude from Step
(a), 0.34 mmol) was dissolved in NH3/MeOH (20 mL)
and the resulting mixture was heated in a pressure Ace
tube at 65 ꢁC for 18 h. The volatiles were evaporated
and the residue was column chromatographed (hexanes/
EtOAc, 8:2 ! 3:7) to give (E)-39 (20 mg, 29% from 23)
and (Z)-39 (33 mg, 48% from 23). Compound (E)-39
diethyl
fluoro(phenylsulfonyl)methylphosphonate20
(297 mg, 0.96 mmol) and LHMDS (0.96 mL, 0.96 mmol)
as described for 20, gave 35 (150 mg, 48%; E/Z, 2:1) as an
inseparable mixture of isomers. Compound (E)-35 had:
1H NMR d 1.30 and 1.47 (2· s, 2 · 3, 2· CH3), 1.71
0
(ddd, J3-4 = 10.9 Hz, J3-3 = 15.5 Hz, J3-2 = 4.6 Hz, 1,
H3), 2.28 (dd, J3 -4 = 4.5 Hz, J3 -3 = 13.4 Hz, 1, H30),
4.77 (t, J2-1/3 = 4.0 Hz, 1, H2), 4.93-4.97 (m, 1, H4),
5.85 (d, J1-2 = 3.6 Hz, 1, H1), 6.31 (dd, J5-F = 32.4 Hz,
J5-4 = 7.5 Hz, 1, H5), 7.56–7.97 (m, 5, Ar); 13C NMR d
0
0
4
1
26.42 and 27.00 (CMe2), 39.25 (d, J3-F = 2.0 Hz, C3),
71.26 (d, J4- F = 2.4 Hz, C4), 80.69 (C2), 105.90 (C1),
had: H NMR d 1.35 and 1.58 (2· s, 2 · 3, 2· CH3),
3
1.78 (br s, 1, OH3), 4.32 (d, J3-4 = 2.5 Hz, 1, H3), 4.59
(d, J2-1 = 3.7 Hz, 1, H2), 4.69 (‘dm’, J4-5 = 7.0 Hz, 1,
H4), 5.53 (ddd, J5-F = 18.1 Hz, J5-6 = 11.2 Hz, J5-4 =
2
112.01 (CMe2), 116.77 (d, J5-F = 3.7 Hz, C5), 129.17
(Ph), 129.91 (ph), 135.08 (Ph), 137.22 (Ph), 155.54 (d,
1J6-F = 301.9 Hz, C6); 19F NMR d ꢁ 122.72 (d,
JF-H5 = 32.5 Hz, 0.66). Compound (Z)-35 had: 1H
NMR d 1.34 and 1.60 (2· s, 2 · 3, 2· CH3), 1.68 (ddd,
7.1 Hz, 1, H5), 5.94 (d, J1-2 = 3.7 Hz, 1, H1), 6.86 (ddd,
J6-F = 82.9 Hz, J6-5 = 11.2 Hz, J6-4 = 1.0 Hz, 1, H6); 13C
4
NMR d 26.47 and 27.06 (CMe2), 76.48 (d, J3-F
2.0 Hz, C3), 76.80 (d, J4-F = 12.6 Hz, C4), 85.31 (C2),
=
3
0
J3-4 = 10.5 Hz, J3-3 = 15.2 Hz, J3-2 = 4.7 Hz, 1, H3),
2
104.87 (C1), 106.20 (d, J5-F = 13.7 Hz, C5), 112.25
2.46 (ddd, J3 -4 = 4.6 Hz, J3 -3 = 13.2 Hz, 1, H30), 4.79
0
0
1
(t, J2-1/3 = 3.9 Hz, 1, H2), 5.71 (ddd, J4-5 = 8.7 Hz, J4-3
=
(CMe2), 153.79 (d, J6-F = 262.6 Hz, C6); 19F NMR d
0
10.6 Hz, J4-3 = 4.5 Hz, 1, H4), 5.84–5.85 (m, 1, H1),
5.86 (dd, J5-F = 20.1 Hz, J5-4 = 8.6 Hz, H5), 7.56–7.97
(m, 5, Ar); 19F NMR d ꢁ114.04 (d, JF-H5 = 20.0 Hz,
0.33); MS m/z 329 (100, MH+).
ꢁ122.18 (dd, JF-H5 = 17.8 Hz, JF-H6 = 82.9 Hz). Com-
pound (Z)-39 had: 1.35 and 1.54 (2· s, 2 · 3, 2· CH3),
1.81 (br s, 1, OH3), 4.22 (br s, 1, H3), 4.58 (d,
J2-1 = 3.7 Hz, 1, H2), 5.07 (ddd, J5-F = 40.1 Hz,
J5-6 = 4.9 Hz, J5-4 = 7.5 Hz, 1, H5), 5.12–5.15 (m, 1,
5.23. (E/Z)-3,5,6-Trideoxy-6-fluoro-1,2-O-isopropylidene-
6-tributylstannyl-a-D-erythro-hex-5-enofuranose (38)
H4), 5.96 (d, J1-2 = 3.7 Hz, 1, H1), 6.63 (dd, J6-F =
82.7 Hz, J6-5 = 4.8 Hz, J6-4 = 1.2 Hz, 1, H6); 13C NMR d
26.57 and 27.10 (CMe2), 74.49 (d, J4-F = 5.1 Hz, C4),
3
4
76.74 (d, J3-F = 1.9 Hz, C3), 85.47 (C2), 104.80 (C1),
Treatment of 35 (128 mg, 0.39 mmol) with Bu3SnH
(0.21 mL, 228 mg, 0.78 mmol,) and AIBN (481 mg,
0.29 mmol) as described for 23, gave 38 (83 mg, 44%;
E/Z, 1:1): 19F NMR d ꢁ92.83 (d, JF-H5 = 33.9 Hz, 84%
1
106.24 (C5), 112.24 (CMe2), 150.20 (d, J6-F = 265.2 Hz,
C6); 19F NMR d ꢁ121.02 (dd, JF-H5 = 41.1 Hz, JF-H6
=
83.3 Hz). MS (APCI+) m/z 205 (100, MH+). Anal. Calcd
for C9H13FO4 (204.19): C, 52.94; H, 6.42. Found: C,
53.19; H, 6.63.
of 0.50F, Z), ꢁ92.83 (ddd, JF-Sn = 230.4 Hz, JF-H5
=
34.7 Hz, JF-H4 = 5.2 Hz 16% of 0.50F, Z), ꢁ96.75 (d,

5100
S. F. Wnuk et al. / Bioorg. Med. Chem. 16 (2008) 5090–5102
5.25. (E/Z)-5,6-Dideoxy-6-fluoro-1,2-O-isopropylidene-a-
D-ribo-hex-5-enofuranose (40)
5.27. (E)-5,6-Dideoxy-6-fluoro-a/b-D-xylo-hex-5-enofura-
nose (42)
Step (a). Compound 24 (200 mg, 0.34 mmol; E/Z, 1:1)
was dissolved in NH3/MeOH (20 mL) and stirred over-
night at ambient temperature. The volatiles were evapo-
rated to give 37 in quantitative yield of sufficient purity
for use in the subsequent step. Step (b). Treatment of
37 (crude, 0.34 mmol)with NH3/MeOH (20 mL) at
65 ꢁC, as described for 39, gave unchanged 37 (17 mg,
10% from 24; E/Z, 2:3) and 40 as separable isomers (E;
22 mg, 32% from 24) and (Z; 18 mg, 26% from 24). Com-
pound (E)-40 had: 1H NMR d 1.40 and 1.60 (2· s, 2 · 3,
2· CH3), 2.37 (d, JOH3-3 = 10.0 Hz, 1, OH3), 3.70–3.73
(m, 1, H3), 4.12 (t, J4-5/3 = 8.3 Hz, 1, H4), 4.60 (t,
A solution of (E)-39 (13 mg, 0.064 mmol) in TFA/H2O
(9:1; 3 mL) was stirred for 50 min at 0 ꢁC (ice bath).
The volatiles were evaporated, coevaporated [toluene
(3·) and MeCN (2·)], and the residue was flash
column chromatographed (50 ! 95% EtOAc/hexanes)
1
to give 42 (4 mg, 38%; a/b, 1:1): H NMR (MeOH-d4)
d 3.91–4.03 (m, 2 H2/3), 4.56–4.63 (m, 1, H4), 5.07 (s,
0.5, H1b), 5.37 (d, J1-2 = 4.0 Hz, 0.5, H1a), 5.51
(ddd, J5-F = 17.8 Hz, J5-6 = 11.2 Hz, J5-4 = 8.9 Hz, 0.5,
H5), 5.65 (ddd, J5-F = 17.9 Hz, J5-6 = 11.1 Hz,
J5-4 = 8.9 Hz, 0.5, H5), 6.87 (dd, J6-F = 84.0 Hz,
J6-5 = 11.0 Hz, 0.5, H6), 6.90 (dd, J6-F = 83.7 Hz,
J6-5 = 11.0 Hz, 0.5, H6); 13C NMR (MeOH-d4) d
J2-1/3 = 4.6 Hz, 1, H2), 5.53 (ddd, J5-F = 17.1 Hz, J5-6 =
11.2 Hz, J5-4 = 7.8 Hz, 1, H5), 5.84 (d, J1-2 = 3.9 Hz, 1,
3
75.29 (d, J4- F = 13.8 Hz, C4), 77.00 and 77.20 (C3),
77.73 (C2), 78.17 (d, J4-F = 13.7 Hz, C4), 81.26 (C2),
3
H1), 6.82 (ddd, J6-F = 82.4 Hz, J6-5 = 11.1 Hz, J6-4
=
0.7 Hz, 1, H6); 13C NMR d 26.75 and 26.84 (CMe2),
76.55 (d, J4-F = 17.0 Hz, C4), 76.49 (C3), 78.57 (C2),
96.72 (C1a), 103.17 (C1b), 108.71 (d, J5-F = 12.0 Hz,
C5), 109.32 (d, J5-F = 11.7 Hz, C5), 152.14 (d,
2
3
2
2
104.08 (C1), 109.44 (d, J5-F = 12.9 Hz, C5), 113.11
1
1J6-F = 258.7 Hz, C6), 152.24 (d, J6-F = 259.2 Hz,
1
(CMe2), 152.81 (d, J6-F = 262.1 Hz, C6); 19F NMR d
C6); 19F NMR (MeOH-d4)d ꢁ126.55 (dd, JF-H5
=
=
ꢁ123.67 (dd, JF-H5 = 17.1 Hz, JF-H6 = 82.5 Hz). Com-
17.8 Hz, JF-6 = 83.9 Hz, 0.5F), ꢁ126.85 (dd, JF-H5
pound (Z)-40 had: 1.40 and 1.60 (2· s, 2 · 3, 2· CH3),
18.1 Hz, JF-H6 = 84.0 Hz, 0.5F); MS (APCIꢁ) m/z 163
2.39 (d, JOH3-3 = 11.0 Hz, 1, OH3), 3.74 (ddd, J3-OH3
=
(100, MHꢁ).
10.9 Hz, J3-4 = 8.9 Hz, J3-2 = 5.1 Hz, 1, H3), 4.60 (t,
J2-1/3 = 4.5 Hz, 1, H2), 4.70 (t, J4-3/5 = 8.8 Hz, 1, H4),
4.89 (ddd, J5-F = 40.0 Hz, J5-6 = 4.9 Hz, J5-4 = 8.9 Hz,
1, H5), 5.84 (d, J1-2 = 3.9 Hz, 1, H1), 6.69 (ddd,
Analogous treatment of 39 (E/Z, 1:1; 20 mg, 0.040 mmol)
gave 42 (5 mg, 76%) as a mixture (E/Z, ꢀ1:1; a/b, ꢀ1:1).
Compound (E/Z)-37 had: 19F NMR (MeOH-d4)
d
J6-F = 82.7 Hz, J6-5 = 4.9 Hz, J6-4 = 0.8 Hz, 1, H6); 13C
ꢁ126.55 (dd, JF-H5 = 17.3 Hz, JF- H6 = 84.0 Hz; E, 0.25,
3
3
2
NMR d 26.79 and 26.92 (CMe2), 73.18 (d, J4-F
=
5.1 Hz, C4), 76.74 (d, J3-F = 2.0 Hz, C3), 78.58 (C2),
a), ꢁ126.85 (dd, JF-H5 = 17.5 Hz, JF-H6 = 84.0 Hz; E,
4
0.25, b), ꢁ 127.66 (dd, JF-H5 = 41.5 Hz, JF-H6
=
104.20 (C1), 108.69 (2J5-F = 1.9 Hz, C5), 113.17
84.9 Hz; Z, 0.25, a), ꢁ 128.34 (dd, JF-H5 = 42.2 Hz,
JF-H6 = 84.8 Hz; Z, 0.25, b); MS(APCIꢁ) m/z 163 (100,
MHꢁ).
1
(CMe2), 153.71 (d, J6-F = 265.8 Hz, C6); 19F NMR d
ꢁ123.90 (dd, JF-H5 = 40.1 Hz, JF-H6 = 82.6 Hz): MS
(APCI+) m/z 205 (100, MH+). Anal. Calcd for
C9H13FO4 (204.19): C, 52.94; H, 6.42. Found: C,
53.07; H, 6.67.
Treatment of the crude 36 [from Step (a) for the prepa-
ration of 39] with TFA/H2O (1 h, 0 ꢁC) followed by
evaporation, coevaporation [toluene (2·) and MeCN
(1·)], partition (H2O/ethyl ether), and evaporation of
the aqueous layer also gave 42 (55% from 23, a/b, 1:1).
5.26. (E/Z)-3,5,6-Trideoxy-6-fluoro-1,2-O-isopropylidene-
a-D-erythro-hex-5-enofuranose (41)
5.28. (E)-5,6-Dideoxy-6-fluoro-a/b-D-ribo-hex-5-enof-
uranose (43)
Treatment of 38 (100 mg, 0.21 mmol; E/Z, 1:1)with
NH3/MeOH (15 mL) and CsF (51 mg, 0.33 mmol) at
65 ꢁC for 4 h, as described for 39 (Step (b)), gave 41
(16 mg, 40%; E/Z, ꢀ45:55): 19F NMR d ꢁ124.79 (dd,
JF-H5 = 41.8 Hz, JF-H6 = 83.0 Hz, 0.55F), ꢁ125.95 (dd,
JF-H5 = 16.7 Hz, JF-H6 = 82.9 Hz, 0.45F); MS (APCI+)
Treatment of (E)-40 (13 mg, 0.064 mmol)with TFA/H2O
(9:1, 3 mL) as described for 42, gave 43 (7 mg, 67%; a/b,
1
1:4): H NMR (MeOH-d4) d 3.77 (t, J3-2/4 = 6.1 Hz, 0.2,
H3), 3.87 (d, J2-1 = 4.5 Hz, 0.8, H2), 4.01–4.05 (m, 1,
H2a and H3b), 4.20 (t, J4-3/5 = 8.0 Hz, 0.8, H4), 4.30
(dd, J4-5 = 8.2 Hz, J4-3 = 6.4 Hz, 0.2, H4), 5.12 (br s,
1
m/z 189 (100, MH+). Compound (E)-41 had: H NMR
d 1.35 and 1.55 (2· s, 2 · 3, 2· CH3), 1.55–1.68 (m, 1,
H3), 2.20 (dd, J3 -3 = 13.5 Hz, J3 -4 = 4.3 Hz, 1, H30),
0
0
0.8, H1), 5.28 (d, J1-2 = 4.1 Hz, 0.2, H1), 5.42 (ddd, J5-F
=
0
4.62 (ddd, J4-3 = 11.5 Hz, J4-5 = 8.2 Hz, J4-3 = 4.3 Hz,
17.5 Hz, J5-6 = 11.1 Hz, J5-4 = 8.3 Hz, 0.2, H5), 5.49
(ddd, J5-F = 17.6 Hz, J5-6 = 11.1 Hz, J5-4 = 8.5 Hz, 0.8,
H5), 6.86 (dd, J6-F = 83.9 Hz, J6-5 = 11.1 Hz, 0.8, H6),
6.87 (dd, J6-F = 83.7 Hz, J6-5 = 11.0 Hz, 0.2, H6);
13C NMR (MeOH- d4) d 70.90 (C2a), 75.32 (d,
1, H4), 4.75–4.79 (m, 1, H2), 5.42 (ddd, J5-F = 16.8 Hz,
J5-6 = 11.2 Hz, J5-4 = 8.3 Hz, 1, H5), 5.82-5.84 (m, 1,
H1), 6.80 (dd, J6-F = 82.7 Hz, J6-5 = 11.2 Hz, 1, H6).
Compound (Z)-41 had: 1.28 and 1.57 (2· s, 2 · 3, 2·
4
4J3-F = 2.6 Hz, C3a), 75.5 (d, J3-F = 2.5 Hz, C3b),
0
CH3), 1.55–1.68 (m, 1, H3), 2.28 (dd, J3 -3 = 13.5 Hz,
J3 -4 = 4.3 Hz, 1, H30), 4.75–4.79 (m, 1, H2), 4.92 (ddd,
J5-F = 41.3 Hz, J5-4 = 8.1 Hz, J5-6 = 4.8 Hz, 1, H5), 5.13
3
0
76.13 (C2b), 77.55 (d, J4-F = 13.6 Hz, C4a), 77.88 (d,
3J4-F = 13.7 Hz, C4b), 96.63 (C1a), 102.09 (C1b),
2
2
0
(ddd, J4-3 = 11.5 Hz, J4-5 = 8.0 Hz, J4-3 = 4.1 Hz, 1,
H4), 5.82–5.84 (m, 1, H1), 6.53 (dd, J6-F = 82.8 Hz,
J6-5 = 4.8 Hz, 1, H6).
111.19 (d, J5-F = 11.4 Hz, C5a), 112.65 (d, J5-F
=
1
10.6 Hz, C5b), 151.98 (d, J6-F = 258.6 Hz, C6b),
152.17 (d, J6-F = 258.7 Hz, C6a); 19F NMR (MeOH-
1

S. F. Wnuk et al. / Bioorg. Med. Chem. 16 (2008) 5090–5102
5101
d4) d ꢁ128.55 (dd, JF-H5 = 17.4 Hz, JF-H6 = 83.5 Hz,
spectrophotometer at room temperature. The initial
rates recorded from the early regions of the progress
curves were fitted into the Lineweaver–Burk equation
1/V = KM0/(kcat [E]0) · 1/[S] + 1/(kcat [E]0) and the
Michaelis–Menten equation V = kcat [E]0 [S]/(KM0 + [S])
using KaleidaGraph 3.5 to determine the inhibition pat-
tern. KI values were calculated from the equation
0.2F, a), ꢁ129.00 (dd, JF-H5 = 17.3 Hz, JF-H6
83.7 Hz, 0.8F, b); MS (APCIꢁ) m/z 163 (100, MHꢁ).
=
Analogous treatment of 40 (E/Z, 1:1; 16 mg,
0.032 mmol) gave 43 (3 mg, 57%) as a mixture (E/Z,
ꢀ3:1; a/b, ꢀ1:4 for E isomer and a/b, ꢀ1:15 for Z iso-
mer): 19F NMR (MeOH-d4)
d
ꢁ128.01 (dd,
KM = KM · (1 + [I]/KI), where KM = 2.2 lM.
0
JF-H5 = 41.3 Hz, JF-H6 = 83.4 Hz; Z, 0.02F, a) ꢁ128.55
(dd, JF-H5 = 17.6 Hz, JF-H6 = 84.2 Hz; E, 0.14F, a),
ꢁ129.00 (dd, JF-H5 = 17.5 Hz, JF-H6 = 83.8 Hz; E,
0.60F, b), ꢁ 129.69 (dd, JF-H5 = 40.8 Hz, JF-H6
= 84.4 Hz; Z, 0.24F, b); MS(APCIꢁ) m/z 163 (100,
MHꢁ).
Acknowledgments
This work was partially supported by grants from the
National Institutes of Health (S06GM08205 and
R01AI062901). C.A.G and J.R were sponsored by
MBRS RISE program (NIH/NIGMS; R25GM61347).
C.A.G is also thankful to R.E. McNair Program for
summer support. The support of US Army Research Of-
fice (W911NF-04-1-0022) in the purchase of 600 MHz
NMR spectrometer is gratefully acknowledged.
Treatment of the crude 37 [from Step (a) for the prepa-
ration of 40] with TFA/H2O (1 h, 0 ꢁC) followed by
evaporation, coevaporation [toluene (2·) and MeCN
(1·)], partition (H2O/ethyl ether), and evaporation of
the aqueous layer also gave 43 (45% from 24, a/b, 1:3).
5.29. (E/Z)-3,5,6-Trideoxy-6-fluoro-a-D-erythro-hex-5-
enofuranose (44)
References and notes
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ethyl ether), and evaporation of the aqueous layer gave
44 (10 mg, 52%; E/Z ꢀ1:3, a/b, ꢀ1:4): 1H NMR (D2O) d
1.85–2.10 (m, 2, H3,30), 4.05–4.25 (m, 1, H2), 4.58–4.75
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=
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17.6 Hz, J5-6 = 11.1 Hz, J5-4 = 9.1 Hz, 0.05, H5), 6.52
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5.30. Enzymatic assay
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