Bioorganic and Medicinal Chemistry p. 1259 - 1264 (2010)
Update date:2022-08-04
Topics: Synthesis Purification Chemical Reaction Enzyme Chemo-enzymatic synthesis
Tomabechi, Yusuke
Suzuki, Rena
Haneda, Katsuji
Inazu, Toshiyuki
Artificial insulin with an N-linked oligosaccharide was synthesized by a chemo-enzymatic method using endo-β-N-acetylglucosaminidase from Mucor hiemalis (Endo-M). GlcNAc-modified insulin was prepared by the reaction of the carboxymethyl glycoside of GlcNA
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