Convenient Synthesis of Oligoribonucleotides Having 2'-5' and/or 3'-5' Phosphodiester Linkage

Article abstract of DOI:10.1246/cl.1990.959

Sequence-defined oligoribonucleosides having 2'-5' and/or 3'-5' phosphodiester linkage have been synthesized by use of 5'-protected ribonucleoside 2',3'-cyclic phosphoramidite on solid support.In coupling reaction, pKa of azole as a catalyst governs the activation.The resulting 2'-5' and/or 3'-5' linked oligoribonucleotides were easily separated by reversed-phase chromatography.