Erratum: Silver nanoassemblies constructed from boranephosphonate DNA (Journal of the American Chemical Society (2013) 135 (6234-6241) DOI: 10.1021/ja400898s)

Article abstract of DOI:10.1021/jacs.7b08409

The following correction is required for the DNA sequences used to prepare the A-tile as used in these arrays. On page S22 of the Supporting Information, a typographical error within the sequences of DNA oligomers A4 and A5 used for the construction of th

Full text of DOI:10.1021/jacs.7b08409

Addition/Correction
pubs.acs.org/JACS
Correction to “Silver Nanoassemblies Constructed from
Boranephosphonate DNA”
Subhadeep Roy, Magdalena Olesiak, Shiying Shang, and Marvin H. Caruthers*
J. Am. Chem. Soc. 2013, 135, 6234−6241. DOI: 10.1021/ja400898s
S
* Supporting Information
2. Update regarding oxidation using tert-butyl hydroperoxide:
As noted in the Supporting Information (Table S1), our
solid-phase syntheses of oligomers containing mixed
phosphate and boranephosphonate linkages used a 15 s
treatment with 1.0 M solution of tert-butyl hydro-
peroxide. While other protocols using tert-butyl hydro-
peroxide have been reported in the literature, these
conditions were chosen to minimize possible oxidation of
the borane group. However, since the publication of the
article, we have tested longer times, up to 60 s, and have
found no effect on BH3.
The following correction is required for the DNA sequences
used to prepare the A-tile as used in these arrays.
On page S22 of the Supporting Information, a typographical
error within the sequences of DNA oligomers A4 and A5 used
for the construction of the DNA arrays led to an insertion of an
extra base pair in the A-tile. These were typographical errors
and were not present in the DNAs used to construct the arrays.
The correct sequences are given here and in the corrected
Supporting Information:
A1: GATGGCGACATCCTGCCGCTATGATTACACAG-
CCTGAGCATTGACAC
A2: GTAGCGCCGTTAGTGGATGTC
A3: TGTAGTATCGTGGCTGTGTAATCATAGCGGCA-
CCAACTGGCA
A4: GACTGCGTGTCAATGCTCACCGATCAACCAG
A5: CTGACGCTGGTTGATCGGACGATACTACATGC-
CAGTTGGACTAACGG
B1a: CAGTGACCGCATCGGACAGCAGC‑T
B1b: CGCTACCGTGCATCATGGACTAAC
ASSOCIATED CONTENT
* Supporting Information
The Supporting Information is available free of charge on the
ACS Publications website at DOI: 10.1021/jacs.7b08409.
■
S
Detailed synthetic procedures, characterization data for
compounds reported, HPLC and MALDI-TOF data for
phosphate DNA sequences synthesized using BIBS-
phosphoramidites as well as additional AFM and TEM
images are provided (corrected) (PDF)
B2: CGTCAGGCTGCTGTGGTCGTGC
B3: AGTACAACGCCACCGATGCGGTCACTGGTTA-
GTGGATTGCGT
B4: GCCATCCGTCGATACGGCACCATGATGCACG
B5: GCAGTCGCACGACCTGGCGTCTGTTGGCTTT-
TGCCAACAGTTTGTACTACGCAATCCTGCCGT-
ATCGACG
REFERENCES
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(1) Winfree, E.; Liu, F.; Wenzler, L. A.; Seeman, N. C. Nature 1998,
394, 539−544.
The sequences reported by Winfree et al.1 were used. In B1a,
an extra thymidine residue at the 3′ end was added as a
convenience for synthesis of boranephosphonate DNA. It
allowed the use of commercially available solid support linked
2′-deoxythymidine. The quality of the arrays was not altered by
the addition of this terminal nucleotide. We note in the Winfree
et al. manuscript as referenced here that hairpin looped
structures extending above/below the arrays were added
without affecting the tiles.
The following comments are added in order to clarify various
questions regarding the original publication:
1. We note that the observed differences between
theoretical and observed masses of bpDNA oligomers
in MALDI-TOf data were due to the fact that the
MALDI-TOF spectra of longer (>10−12 mers) bpDNA
oligomers in our hands yielded very broad peaks. The
mass provided in the original paper is what we estimated
to be the center of these broad peaks. These data should
therefore be used in conjunction with other data such as
the 31P NMR and gel analysis for complete character-
ization of these oligomers.
© XXXX American Chemical Society
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DOI: 10.1021/jacs.7b08409
J. Am. Chem. Soc. XXXX, XXX, XXX−XXX