Synthetic models related to methoxalen - CYP2A6 interactions. Dimethoxybenzofuran derivatives as potent and selective inhibitors of cyp2a6

Article abstract of DOI:10.3987/COM-13-12744

The human CYP2A6 enzyme metabolizes several xenobiotics including nicotine, the addictive component in tobacco. Reduced activity of CYP2A6, either for genetic reasons or by administering inhibitors of CYP2A6, reduces tobacco smoking. The reported compound

Full text of DOI:10.3987/COM-13-12744

HETEROCYCLES, Vol. 87, No. 8, 2013, pp. -. © 2013 The Japan Institute of Heterocyclic Chemistry
Received, 14th May, 2013, Accepted, 6th June, 2013, Published online, 17th June, 2013
DOI: 10.3987/COM-13-12744
SYNTHETIC MODELS RELATED TO METHOXALEN – CYP2A6
INTERACTIONS. DIMETHOXYBENZOFURAN DERIVATIVES AS
POTENT AND SELECTIVE INHIBITORS OF CYP2A6
Yuki Yamaguchi, Ichie Akimoto, Kyoko Motegi, Teruki Yoshimura, Keiji
Wada, Naozumi Nishizono, and Kazuaki Oda*
School of Pharmaceutical Sciences, Health Sciences University of Hokkaido,
Ishikari-Tobetsu, Hokkaido 061-0293, Japan; e-mail: k-oda@hoku-iryo-u.ac.jp
Abstract – The human CYP2A6 enzyme metabolizes several xenobiotics
including nicotine, the addictive component in tobacco. Reduced activity of
CYP2A6, either for genetic reasons or by administering inhibitors of CYP2A6,
reduces tobacco smoking. The reported compound methoxalen had a potent
inhibitory effect on activity of CYP2A6 with an IC50 value of 1.27 µM. We
selected methoxalen as
dimethoxybenzofuran derivatives that have inhibitory effects on activity of human
cytochrome P450 (CYP) 2A6. Synthetic benzofuran derivatives
a
lead compound and prepared various
(3,6-dimethoxybenzofuran: IC50=1.92 µM and 3,7-dimethoxybenzofuran:
IC50=2.00 µM) also exhibited comparable activities against CYP2A6 and were
selective inhibitors of CYP2A6. These compounds can be used as lead
compounds in the development of drugs for smoking reduction therapy.
Nicotine, the primary component present in tobacco, plays a significant role in establishing and
maintaining tobacco dependence. CYP2A6 is known to be responsible for the metabolism of nicotine to
cotinine in humans, approximately 70-80% of nicotine being metabolized by CYP2A6 to cotinine.1
CYP2A6 does not seem to have an extensive role in human drug metabolism, but it has been shown to be
involved in the mutagenic activation of promutagens such as the tobacco-specific nitrosoamine,
4-(methylnitrosoamino)-1-(3-pyridyl)-1-butanone (NNK).2 It has also been shown that methoxalen
(9-methoxyfuranocoumarin), a potent human CYP2A6 inhibitor, is a strong chemopreventive agent
against NNK induction of lung tumorigenesis.3 Pharmacogenomic studies have suggested that male
smokers completely lacking CYP2A6 were more resistant to lung cancer.4 In addition, inhibition of

CYP-mediated metabolism would lead to slower elimination of nicotine from the bodies of smokers and,
consequently, to a decrease in the number of cigarettes needed to maintain a constant level of nicotine in
the body. This decrease in smoking would decrease the exposure to carcinogenic nitrosoamine.5 For this
reason, CYP2A6 inhibitors have been proposed as novel targets for reducing tobacco-related cancer risk.
An ideal drug candidate for use as a CYP2A6 inhibitor and a smoking cessation agent must be highly
potent and selective to avoid undesirable effects.5
Although a number of compounds, including methoxalen, have been used as inhibitors of CYP2A6, these
compounds generally lack selectivity for targeting CYP2A6 and many of these compounds also inhibit
activities of other drug-metabolizing enzymes (such as CYP3A4) and therefore can result in untoward
drug-drug interactions.6 For example, methoxalen (1) showed an IC50 value for CYP3A4 of 5.46 µm.6
Furthermore, menthofuran ((R)-4,5,6,7-tetrahydro-3,6-dimethylbenzofuran), an excellent selective
inhibitor of CYP2A6, has disadvantage in toxicity.7 We selected a benzofuran ring system that has the
partial structure common to two CYP2A6 inhibitors (methoxalen and menthofuran). Herein we report a
synthesis of dimethoxybenzofuran derivatives that have a simple ring system than that of furanocoumarin
derivatives and present results of assays of their inhibitory effects on CYP2A6 activity with a view to
defining the relationship between structures and inhibitory effects on CYP2A6 activity.
To better understand the binding methoxalen to CYP2A6, Johnson et al. determined the structure of the
enzyme by X-ray crystallography.8 The CYP2A6 structure shows a compact, hydrophobic active site with
one hydrogen bond donor, Asn297. In the crystal structure, Asn297 acts as a hydrogen donor to the
carbonyl oxygen of methoxalen, and the oxygen of the furan ring is closest to the heme iron in the active
site of CYP2A6 for reigioselective oxidation.8ꢀ We also found that bergapten 2 (methoxy group at the
4-position on the furanocoumarin ring) showed a much weaker inhibitory effect on CYP2A6 than that of
methoxalen 1, indicating that the potency of inhibition is also very sensitive to location of the methoxy
group on the furanocoumarin ring.9 We selected methoxalen (1) as a lead compound and prepared various
dimethoxybenzofuran derivatives. We expected that the methoxy group on the benzofuran ring would act
as a hydrogen acceptor of Asn297 instead of carbonyl oxygen of methoxalen (1) (Scheme 1). A series of
benzofuran derivatives (3a ~ 3h) derivatives were prepared as shown in Scheme 2.
Methoxalen (1)
+
-
0.1Å
2.8
O
ꢅ
ꢄ
ꢆ
A
s
n
2
9
7
ꢃ
ꢄ
ꢆ
Me
ꢃ
O
ꢂ
ꢇ
A
s
O
n
2
9
7
ꢂ
ꢅ
MeO
ꢁ
OMe
O
ꢁ
O
ꢀ
ꢀ
+
0.1Å
3.3
-
hydrogen bond
Heme
Heme
Scheme 1

O
O
O
HO
BrCH2CO2Me,
K2CO3
MeO
O
NaOMe
OMe
OMe
O
MeO
OMe
MeO
MeOH,
0 ˚C - reflux
OMe
acetone, reflux
O
OH
63 ~ 84%
71% ~ quant.
MeO
MeO
MeSO4
K2CO3
MeO
O
Cu
K2CO3, H2O
MeOH, reflux
OMe
OMe
HO2C
quinoline, 185 ˚C
acetone, reflux
O
O
94% ~ quant.
R1
3a: R1 = OMe, R2 = R3 = R4 = H (71%)
3b: R1 = R3 = R4 = H, R2 = OMe (91%)
3c: R1 = R2 = R4 = H, R3 = OMe (77%)
3d: R1 = R2 = R3 = H, R4 = OMe (83%)
MeO
R2
R3
O
R4
OMe
OMe
OMe
BrCH2CH=CH2,
K2CO3
200 ˚C
N2
MeO
OH
MeO
Cl
O
acetone, reflux
OMe
MeO
OH
quant.
96%
OMe
ClCH2CH2Cl
tert-BuOK
NaOH, H2O,
n-Bu4NBr, reflux
THF, reflux
MeO
O
MeO
O
53%
86%
OMe
Grubbs catalyst 1st
CH2Cl2, rt.
O
OMe
3f quant.
MeO
BrCH2CH=CH2,
K2CO3
1. mCPBA,
CH2Cl2, reflux
MeO
MeO
O
acetone, reflux
2. NaOH, MeOH
CHO
OH
OMe
OMe
OMe
89% ~ quant.
72 ~ 88%
BrCH2CH=CH2,
K2CO3
MeO
MeO
RuClH(CO)(PPh3)3
200 ˚C
toluene or CH2Cl2,
reflux
acetone, reflux
N2
OH
O
OMe
OMe
93% ~ quant.
R2
62 ~ 80%
R1
MeO
Grubbs catalyst 2nd
toluene, reflux
O
O
OMe
OMe
3g: R1 = OMe, R2 = H (quant.)
3h: R1 = H, R2 = OMe (93%)
81 ~ 95%
Scheme 2
3,4-Dimethoxybenzofuran (3a) was synthesized from methyl 2-hydroxy-6-methoxybenzoate.10 Methyl
2-hydroxy-6-methoxybenzoate was converted into ether using methyl bromoacetate and K2CO3. This
compound treated with sodium methoxide in methanol to give 3-hydroxybenzofuran derivatives in 83%
yield. Reaction of 3-hydroxybrnzofuran with dimethyl sulfate followed by hydrolysis and
copper-catalyzed decarboxylation gave 3,4-dimethoxybenzofuran (3a). Dimethoxybenzofuran derivatives

(3b~d) were similarly prepared. 4,5-Dimethoxybenzofuran (3e) was prepared from
3-hydroxy-4-methoxybenzaldehyde by the reported procedure.11 4,6-Dimethoxybenzofuran (3f) was
synthesized from 3,5-dimethoxyphenol by an isomerization-ring-closing metathesis strategy.12
3,5-Dimethoxyphenol was converted into allyl ether using allyl bromide and K2CO3. Subsequent Claisen
rearrangements gave the corresponding phenol quantitatively. This phenol was alkylated with
1,2-dichloroethane followed by treatment with potassium tert-butoxide to give an aryl enol ether. The
aryl enol ether was cyclized to give 4,7-dimethoxybenzofuran by using first-generation Grubbs
metathesis catalyst. 4,7-Dimethoxybenzofuran (3g) was synthesized from 2,5-dimethoxyphenol (prepared
from 2,5-dimethoxybenzaldehyde by Baeyer-Villiger oxidation) by an isomerization-ring-closing
metathesis strategy.11 2,5-Dimethoxyphenol was converted into allyl ether using allyl bromide and K2CO3.
Subsequent Claisen rearrangements gave the corresponding phenol in good yield. This phenol was
re-allylated to afford 1-allyl-2-allyloybenzene. The two allyl groups were isomerized with
ruthenium-based catalyst [RuClH(CO)(PPh3)3] to give an aryl enol ether.13 This compound was not
characterized further and was used directly in the next reaction. The aryl enol ether was cyclized to give
4,7-dimethoxybenzofuran quantitatively by using the second-generation Grubbs metathesis catalyst.
5,7-Dimethoxybenzofuran (3h) was similarly prepared from 2,4-dimethoxyphenol.
Assays of inhibition of CYP2A6 activity by benzofuran derivatives were based on microsomal coumarin
7-hydroxylation.14 The results are shown in Scheme 3 and Table 1.
Table 1. IC50 Values of Benzofuran Derivatives
for Coumarin 7-Hydroxylation
MeO
3a: 3,4-dimethoxy
3b: 3,5-dimethoxy
3c: 3,6-dimethoxy
3d: 3,7-dimethoxy
OMe
Compounds
CYP2A6 IC50 (µM)
O
Methoxalen
1.27
> 10
OMe
3a
3e: 4,5-dimethoxy
3f: 4,6-dimethoxy
3g: 4,7-dimethoxy
3b
3c
3d
3e
3f
> 10
1.92
2.00
7.51
> 10
9.29
5.16
OMe
OMe
O
3h: 5,7-dimethoxy
O
3g
3h
OMe
Scheme 3
At first, the inhibitory activities of 3-methoxybenzofuran derivatives (3a, 3b, 3c, and 3d) were compared.
Introduction of a methoxy group at the 6 (3c) or 7 (3d) position on the benzofuran ring resulted in
significantly increased inhibitory potency (1.92 µM and 2.00 µM, respectively), whereas the same
substituent in the 4 (3a) or 5 (3b) position gave rise to low potency (> 10 µM) of CYP2A6 inhibition.
Next, the inhibitory activities of 4-methoxybenzofuran derivatives (3e, 3f, and 3g) were examined.

Though 3e and 3f showed moderate potency of CYP2A6 inhibition (7.51 µM and 9.29 µM, respectively),
none of the derivatives exhibited activities comparable to those of 3c and 3d against CYP2A6, indicating
that the existence of a methoxy group at the 3-position of benzofuran is important for potency of
CYP2A6 inhibition. 4,6-Dimethoxybenzofuran (3f) showed poor inhibitory activity against CYP2A6.
Finally, CYP2A6 inhibition by 5,7-dimethoxybenzofuran (3h) was studied. 5,7-Dimethoxybenzofuran
(3h) also showed moderate inhibitory activity against CYP2A6. It was found that the potency of
inhibition was very sensitive to location of the methoxy group on the benzofuran ring.
In this experiment, 8 candidate molecules were selected and tested for inhibition potency.
3,6-Dimethoxyethoxybenzofuran 3c and 3,7-dimethoxybenzofuran 3d were most potent as CYP2A6
inhibitors with IC50 values of 1.92 µM and 2.00 µM, respectively. These results and our findings provide a
clue for elucidating the relationship between structures and inhibitory effects on CYP2A6 activity. As
previously described, in the crystal structure, Asn297 acts as a hydrogen donor to the carbonyl oxygen of
methoxalen, and the oxygen of the furan ring is closest to the heme iron in the active site of CYP2A6.7 A
hydrogen bond with Asn297 and van der Waals interaction between the hydrophobic amino acids and
inhibitor are important determinants of inhibition potency. We speculated that the presence of a hydrogen
bond between a 6- or 7-methoxy group on the benzofuran ring and Asn297 in the active site of CYP2A6
has an effect on inhibition potency. Furthermore, Rahnasto et al. reported that the crystal structure shows
that electron-rich regions (furan ring of methoxalen) in suicide inhibitors coordinate to the heme iron.15ꢀ
Thus, the 3-methoxy group, an electron-donating substituent, on the furan ring in 3c and 3d is also a
determinant of inhibition potency.
In order to further explore the selectivity of CYP 2A6 inhibition, compounds 3c and 3d ! a selection of
the most potent inhibitors of CYP2A6 among compounds examined in this study – were tested for their
inhibitory potency for the most prominent drug-metabolizing enzyme CYP3A4. Both of them (3c and 3d)
showed IC50 values on CYP3A4 of > 10 µm.16
A number of compounds, including methoxalen, have been reported to be inhibitors of CYP2A6.
However, these compounds generally lack selectivity for targeting CYP2A6, and many also inhibit other
drug-metabolizing enzymes such as CYP3A4A.6 Fortunately, of the compounds examined, compounds
3c and 3d were found to have highly selective inhibitory potency for CYP2A6. These compounds may
serve as lead structures to develop even more potent and selective inhibitors of CYP2A6 that are more
stable and accessible than methoxalen and have comparable activity against CYP2A6.
There is considerable interest in development of clinically useful inhibitors of the CYP2A6 enzyme.
None of the currently known CYP2A6 inhibitors are suitable for routine clinical use, and thus there is a
need to develop novel inhibitors with suitable potency, selectivity and toxicity profiles. The
dose-response relationship between the number of cigarettes smoked and development of related cancer
was established many years ago by epidemiological studies.17 Reducing the number of cigarettes smoked

and decreasing the toxicity and carcinogenicity of tobacco will result in less tobacco-related cancer risk.
EXPERIMENTAL
All melting points were determined using a Yamato melting point apparatus (MP-J3) and are uncorrected.
NMR spectra were obtained using JEOL JNM-ECA 500 spectrometers. The chemical shifts are reported
in ppm (") relative to TMS (0.0 ppm) as the internal standard. MS spectra (MS, HRMS) were obtained
using a Shimadzu GC MS 9100-MK gas chromatograph-mass spectrometer. Column chromatography
was conducted using silica gel (Merck, Kieselgel 60, 70-230 mesh).
Preparation of inhibitors
General procedure for preparation of 3-methoxybenzofuran derivatives (3a, 3b, 3c, and 3d)
Appropriate phenols and methyl bromoacetate were refluxed for 4 h in the presence of K2CO3 to give
diesters.
1
Methyl 2-methoxy-6-(2-methoxy-2-oxoethoxy)benzoate. mp 63-64 ºC. H-NMR (500 MHz, CDCl3) ":
3.77 (3H, s), 3.82 (3H, s), 3.92 (3H, s), 4.64 (2H, s), 6.43 (1H, d, J = 8.0 Hz), 6.60 (1H, d, J = 8.0 Hz),
13
7.26 (1H, t, J = 8.0 Hz). C-NMR (125 MHz, CDCl3) !: 52.3, 52.6, 56.2, 66.3, 105.1x2, 114.0, 131.2,
155.8, 157.6, 166.6, 169.1. ESI-LRMS m/z: 277 (MNa+). ESI-HRMS m/z: 277.0689 (Calcd for
C12H14NaO6: 277.0688).
1
Methyl 5-methoxy-2-(2-methoxy-2-oxoethoxy)benzoate. mp 81-83 ºC (lit.17 82-85 ºC). H-NMR (500
MHz, CDCl3) !: 3.79 (6H, s), 3.90 (3H, s), 4.66 (2H, s), 6.91 (1H, d, J = 9.2 Hz), 6.99 (1H, dd, J = 2.9,
13
9.2 Hz), 7.35 (1H, d, J = 2.9 Hz). C-NMR (125 MHz, CDCl3) !: 52.3, 52.4, 55.9, 68.4, 116.0, 117.8,
119.7, 122.4, 151.8, 154.5, 166.2, 169.5. ESI-LRMS m/z: 277 (MNa+). Its spectral data are in accordance
with previously reported data.18
1
Methyl 4-methoxy-2-(2-methoxy-2-oxoethoxy)benzoate. Yellow oil. H-NMR (500 MHz, CDCl3) !:
3.80 (3H, s), 3.82 (3H, s), 3.86 (3H, s), 4.70 (2H, s), 6.39 (1H, d, J = 2.3 Hz), 6.56 (1H, dd, J = 2.3, 9.2
Hz), 7.88 (1H, d, J = 9.2 Hz). 13C-NMR (125 MHz, CDCl3) !: 51.9, 52.4, 55.6, 66.7, 101.4, 106.3, 113.4,
134.2, 159.6, 164.1, 165.9, 169.0. Its spectral data are in accordance with previously reported data.19
1
Methyl 3-methoxy-2-(2-methoxy-2-oxoethoxy)benzoate. mp 71-73 ºC (lit.,18 75.5 ºC). H-NMR (500
MHz, CDCl3) !: 3.80 (3H, s), 3.85 (3H, s), 3.88 (3H, s), 4.68 (2H, s), 7.05 (1H, dd, J = 1.8, 8.0 Hz), 7.11
(1H, t, J = 8.0 Hz), 7.31 (1H, dd, J = 1.8, 8.0 Hz). 13C-NMR (125 MHz, CDCl3) !: 52.1, 52.4, 56.2, 69.9,
115.9, 122.3, 124.6, 126.5, 146.7, 153.1, 166.6, 169.8. EI-LRMS m/z: 254 (M+). Its spectral data are in
accordance with previously reported data.20
Diester and NaOMe were refluxed in MeOH to give 3-hydroxybenzofuran derivatives. These compounds
were not characterized further and were used directly in the next reaction. 3-Hydroxybenzofuran
derivatives and Me2SO4 were refluxed in the presence of K2CO3 to give 3-methoxybenzofuran

derivatives.
1
Methyl 3,4-dimethoxybenzofuran-2-carboxylate. mp 77-78 ºC (lit.,9 70 ºC). H-NMR (500 MHz,
CDCl3) !: 3.95 (3H, s), 3.96 (3H, s), 4.10 (3H, s), 6.65 (1H, d, J = 8.0 Hz), 7.09 (1H, d, J = 8.6 Hz), 7.35
13
(1H, dd, J = 8.0, 8.6 Hz). C-NMR (125 MHz, CDCl3) !: 52.1, 56.0, 63.2, 103.6, 105.6, 113.0, 129.5,
131.7, 150.0, 154.8, 154.9, 159.8. ESI-LRMS m/z: 259 (MNa+). Its physical and spectral data are in
accordance with previously reported data.1
1
Methyl 3,5-dimethoxybenzofuran-2-carboxylate. mp 106-109 ºC (lit.,10 109 ºC). H-NMR (500 MHz,
CDCl3) !: 3.85 (3H, s), 3.96 (3H, s), 4.23 (3H, s), 7.07 (1H, dd, J = 2.3, 9.2 Hz), 7.12 (1H, d, J = 2.3 Hz),
13
7.38 (1H, d, J = 9.2 Hz). C-NMR (125 MHz, CDCl3) !: 52.1, 56.0, 61.9, 101.8, 113.7, 118.7, 122.6,
131.8, 148.4, 149.9, 156.1, 159.8. ESI-LRMS m/z: 259 (MNa+). Its physical and spectral data are in
accordance with previously reported data.10
1
Methyl 3,6-dimethoxybenzofuran-2-carboxylate. mp 69-70 ºC (lit., 10 70 ºC). H-NMR (500 MHz,
CDCl3) !: 3.85 (3H, s), 3.95 (3H, s), 4.26 (3H, s), 6.89 (1H, dd, J = 2.3, 8.6 Hz), 6.95 (1H, d, J = 2.3 Hz),
7.65 (1H, d, J = 8.6 Hz). 13C-NMR (125 MHz, CDCl3) !: 51.9, 55.8, 61.4, 96.0, 113.7, 115.1, 121.9, 129.7,
150.2, 155.0, 159.9, 161.2. ESI-LRMS m/z: 259 (MNa+). Its physical and spectral data are in accordance
with previously reported data.10
1
Methyl 3,7-dimethoxybenzofuran-2-carboxylate. mp 68-70 ºC (lit.,10 68 ºC). H-NMR (500 MHz,
CDCl3) !: 3.95 (3H, s), 3.99 (3H, s), 4.26 (3H, s), 6.91 (1H, d, J = 8.0 Hz), 7.18 (1H, t, J = 8.0 Hz), 7.34
13
(1H, d, J = 8.0 Hz). C-NMR (125 MHz, CDCl3) !: 52.0, 56.0, 61.6, 109.3, 113.0, 123.6, 123.9, 130.9,
143.1, 146.2, 150.0, 159.8. ESI-LRMS m/z: 259 (MNa+). Its physical and spectral data are in accordance
with previously reported data.10
3-Methoxybenzofuran derivatives and K2CO3 were refluxed in aqueous MeOH to give
benzofuran-2-carboxylic acid derivatives.
1
3,4-Dimethoxybenzofuran-2-carboxylic acid. mp 214-216 ºC (lit.,10 218 ºC). H-NMR (500 MHz,
acetone-d6) !: 3.97 (3H, s), 4.03 (3H, s), 6.81 (1H, d, J = 8.0 Hz), 7.08 (1H, d, J = 8.6 Hz), 7.42 (1H, dd, J
13
= 8.0, 8.6 Hz). C-NMR (125 MHz, acetone-d6) !: 55.4, 62.3, 103.9, 105.0, 112.9, 129.6, 132.3, 149.5,
154.7, 154.9, 159.1. ESI-LRMS m/z: 245 (MNa+). ESI-HRMS m/z: 245.0440 (Calcd for C11H10NaO5:
245.0426). Its physical and spectral data are in accordance with previously reported data.10
1
3,5-Dimethoxybenzofuran-2-carboxylic acid. mp 181-184 ºC (lit.,10 184 ºC). H-NMR (500 MHz,
acetone-d6) !: 2.99 (1H, br s), 3.85 (3H, s), 4.18 (3H, s), 7.10 (1H, dd, J = 2.3, 9.2 Hz), 7.22 (1H, d, J =
13
2.3 Hz), 7.42 (1H, d, J = 9.2 Hz). C-NMR (125 MHz, acetone-d6) !: 55.4, 61.3, 101.7, 113.3, 118.5,
122.8, 132.3, 148.1, 149.5, 156.4, 159.2. ESI-LRMS m/z: 245 (MNa+). Its physical and spectral data are
in accordance with previously reported data.10
1
3,6-Dimethoxybenzofuran-2-carboxylic acid. mp 174-177 ºC (lit.,10 177ºC). H-NMR (500 MHz,
acetone-d6) !: 2.97 (1H, br s), 3.87 (3H, s), 4.21 (3H, s), 6.92 (1H, dd, J = 2.3, 8.6 Hz), 7.05 (1H, d, J =

13
2.3 Hz), 7.72 (1H, d, J = 8.6 Hz). C-NMR (125 MHz, acetone-d6) !: 55.3, 61.0, 95.7, 113.6, 115.2,
121.8, 130.2, 149.9, 154.8, 159.2, 161.4. ESI-LRMS m/z: 245 (MNa+). Its physical and spectral data are
in accordance with previously reported data.10
1
3,7-Dimethoxybenzofuran-2-carboxylic acid. mp 216-218 ºC (lit.,10 216 ºC). H-NMR (500 MHz,
acetone-d6) !: 3.09 (1H, br s), 3.97 (3H, s), 4.20 (3H, s), 7.07 (1H, d, J = 8.0 Hz), 7.22 (1H, t, J = 8.0 Hz),
7.37 (1H, d, J = 8.0 Hz). 13C-NMR (125 MHz, acetone-d6) !: 55.5, 61.2, 109.7, 112.7, 123.9, 124.0, 131.4,
142.9, 146.3, 149.6, 159.2. ESI-LRMS m/z: 245 (MNa+). Its physical and spectral data are in accordance
with previously reported data.10
Dimethoxybenzofuran-2-carboxylic acid derivatives and Cu powder were heated at 185 °C in quinoline to
convert appropriate dimethoxybanzofurans (3a ~ 3d).
3,4-Dimethoxybenzofuran (3a). mp 95-97 ºC (lit.,10 99 ºC). 1H-NMR (500 MHz, CDCl3) !: 3.88 (3H, s),
3.96 (3H, s), 6.63 (1H, d, J = 8.0 Hz), 7.01 (1H, d, J = 8.0 Hz), 7.16 (1H, s), 7.20 (1H, t, J = 8.0 Hz).
13C-NMR (125 MHz, CDCl3) !: 56.0, 58.8, 103.0, 105.2, 111.2, 123.3, 125.8, 145.6, 154.1, 155.4.
EI-LRMS m/z: 178 (M+). Its physical and spectral data are in accordance with previously reported data.10
3,5-Dimethoxybenzofuran (3b). Color less oil. 1H-NMR (500 MHz, CDCl3) !: 3.81 (3H, s), 3.86 (3H, s),
6.89 (1H, dd, J = 2.9, 9.2 Hz), 6.99 (1H, d, J = 2.9 Hz), 7.28 (1H, d, J = 9.2 Hz), 7.49 (1H, s). 13C-NMR
(125 MHz, CDCl3) !: 56.0, 58.2, 100.5, 112.4, 114.4, 122.0, 125.3, 145.6, 148.8, 155.6. EI-LRMS m/z:
178 (M+). Its spectral data are in accordance with previously reported data.10
3,6-Dimethoxybenzofuran (3c). mp 73-76 ºC (lit.,9 76 ºC). 1H-NMR (500 MHz, CDCl3) !: 3.83 (3H, s),
3.85 (3H, s), 6.85 (1H, dd, J = 2.3, 8.6 Hz), 6.90 (1H, d, J = 2.3 Hz), 7.16 (1H, s), 7.43 (1H, d, J = 8.6 Hz).
13C-NMR (125 MHz, CDCl3) !: 55.8, 58.2, 96.1, 111.5, 115.3, 119.0, 123.5, 145.6, 154.9, 158.7.
EI-LRMS m/z: 178 (M+). Its physical and spectral data are in accordance with previously reported data.10
3,7-Dimethoxybenzofuran (3d). mp 42-45 ºC (lit.,10 45 ºC). 1H-NMR (500 MHz, CDCl3) !: 3.86 (3H, s),
3.93 (3H, s), 6.88 (1H, dd, J = 1.7, 6.9 Hz), 7.09-7.13 (2H, m), 7.51 (1H, s). 13C-NMR (125 MHz, CDCl3)
!: 56.1, 58.3, 106.9, 111.1, 123.0, 123.4, 124.7, 143.2, 145.5, 145.8. EI-LRMS m/z: 178 (M+). Its physical
and spectral data are in accordance with previously reported data.10
Procedure for preparation of 4,6-dimethoxybenzofuran (3f)
3,5-Dimethoxyphenol and allyl bromide were refluxed in the presence of K2CO3 for 2 h to give the
allyloxybenzene derivative.
1
1-(Allyloxy)-3,5-dimethoxybenzene. Yellow oil, H-NMR (500 MHz, CDCl3) !: 3.76 (6H, s), 4.49 (2H,
d, J = 5.7 Hz), 5.28 (1H, m), 5.41 (1H, m), 6.01-6.10 (4H, m). 13C-NMR (125 MHz, CDCl3) !: 55.4, 69.0,
93.2, 93.7, 117.9, 133.2, 160.6, 161.6. EI-LRMS m/z: 194 (M+). EI-HRMS m/z: 194.0940 (Calcd for
C11H14O3: 194.0943).
Allyloxybenzene was heated at 200 °C for 45 min to give the 2-allylphenol derivative.
2-Allyl-3,5-dimethoxyphenol. Yellow oil, 1H-NMR (500 MHz, CDCl3) !: 3.38 (2H, dt, J = 1.7, 6.3 Hz),

3.76 (3H, s), 3.77 (3H, s), 5.06-5.14 (3H, m), 5.99 (1H, m), 6.08 (1H, d, J = 2.3 Hz), 6.10 (1H, d, J = 2.3
Hz). 13C-NMR (125 MHz, CDCl3) !: 27.1, 55.4, 55.8, 91.7, 93.8, 105.7, 115.4, 136.9, 156.0, 158.8, 159.8.
EI-LRMS m/z: 194 (M+). EI-HRMS m/z: 194.0945 (Calcd for C11H14O3: 194.0943).
This phenol and 1,2-dichloroethane were refluxed in the presence of TBAB and NaOH for 1.5 h to give
2-allyl-1-(2-chloroethoxy)-3,5-dimethoxybenzene.
1
2-Allyl-1-(2-chloroethoxy)-3,5-dimethoxybenzene. Colorless oil, H-NMR (500 MHz, CDCl3) !: 3.34
(2H, dt, J = 1.7, 6.3 Hz), 3.78-3.80 (8H, m), 4.19 (2H, t, J = 6.0 Hz), 4.89-4.98 (2H, m), 5.92 (1H, m),
6.09 (1H, d, J = 2.3 Hz), 6.16 (1H, d, J = 2.3 Hz). 13C-NMR (125 MHz, CDCl3) !: 27.1, 42.1, 55.5, 55.9,
68.7, 91.6, 91.9, 109.9, 113.9, 137.3, 157.3, 159.0, 159.5. EI-LRMS m/z: 256 (M+). EI-HRMS m/z:
256.0865 (Calcd for C13H17ClO3: 256.0866).
2-Allyl-1-(2-chloroethoxy)-3,5-dimethoxybenzene and potassium tert-butoxide were refluxed in THF for
30 min under N2 to give the propenyl vinylbenzene derivative.
1
(E)-1,5-Dimethoxy-2-(prop-1-en-1-yl)-3-(vinyloxy)benzene. Colorless oil, H-NMR (500 MHz, CDCl3)
!: 1.89 (3H, d, J = 4.6 Hz), 3.78 (3H, s), 3.82 (3H, s), 4.39 (1H, dd, J = 1.8, 6.0 Hz), 4.68 (1H, dd, J = 1.8,
13.8 Hz), 6.17 (1H, d, J = 2.3 Hz), 6.24 (1H, d, J = 2.3 Hz), 6.45-6.47 (2H, m), 6.54 (1H, m). 13C-NMR
(125 MHz, CDCl3) !: 20.1, 55.5, 55.8, 94.2, 94.6, 95.8, 110.5, 120.5, 129.1, 149.0, 155.5, 159.0, 159.1.
EI-LRMS m/z: 220 (M+). EI-HRMS m/z: 220.1100 (Calcd for C13H16O3: 220.1099).
The aryl enol ether and the first-generation Grubbs catalyst (5% mol) were refluxed for 2 h to give
4,7-dimethoxybenzofuran.
4,6-Dimethoxybenzofuran (3f). Colorless oil, 1H-NMR (500 MHz, CDCl3) !: 3.84 (3H, s), 3.90 (3H, s),
13
6.33 (1H, d, J = 2.3 Hz), 6.67 (1H, m), 6.78 (1H, m), 7.46 (1H, d, J = 2.3 Hz). C-NMR (125 MHz,
CDCl3) !: 55.6, 55.8, 88.3, 94.3, 103.9, 111.4, 142.6, 153.7, 156.8, 159.2. EI-LRMS m/z: 178 (M+).
EI-HRMS m/z: 178.0633 (Calcd for C10H10O3: 178.0630).
General procedure for preparation of 7-methoxybenzofuran derivatives (3g and 3h)
Appropriate dimethoxybenzaldehydes and m-chloroperbenzoic acid were refluxed in CH2Cl2 for 14 h to
give dimethoxyphenols.
2,5-Dimethoxyphenol. Colorless oil. 1H-NMR (500 MHz, CDCl3) !: 3.74 (3H, s), 3.84 (3H, s), 5.68 (1H,
s), 6.37 (1H, dd, J = 3.4, 9.2 Hz), 6.56 (1H, d, J = 3.4 Hz), 6.77 (1H, d, J = 9.2 Hz). 13C-NMR (125 MHz,
CDCl3) !: 55.7, 56.7, 101.8, 104.3, 111.5, 141.0, 146.5, 154.6. Its spectral data are in accordance with
previously reported data.21
2,4-Dimethoxyphenol. Colorless oil. 1H-NMR (500 MHz, CDCl3) !: 3.76 (3H, s), 3.86 (3H, s), 5.23 (1H,
s), 6.38 (1H, dd, J = 2.8, 8.6 Hz), 6.49 (1H, d, J = 2.8 Hz), 6.82 (1H, d, J = 8.6 Hz). 13C-NMR (125 MHz,
CDCl3) !: 55.9, 56.0, 99.5, 104.3, 114.2, 139.9, 147.1, 153.6. Its spectral data are in accordance with
previously reported data.22
The corresponding phenols and allyl bromide were refluxed in the presence of K2CO3 for 20 h to give the

allyloxybenzene derivative.
1
2-(Allyloxy)-1,4-dimethoxybenzene. Colorless oil. H-NMR (500 MHz, CDCl3) !: 3.75 (3H, s), 3.83
(3H, s), 4.59 (2H, dt, J = 1.7, 5.2 Hz), 5.27-5.42 (2H, m), 6.07 (1H, m), 6.40 (1H, dd, J = 2.9, 8.6 Hz),
6.52 (1H, d, J = 2.9 Hz), 6.79 (1H, d, J = 8.6 Hz). 13C-NMR (125 MHz, CDCl3) !: 55.7, 56.7, 69.9, 102.2,
103.6, 112.5, 118.1, 133.3, 143.9, 148.9, 154.2. Its spectral data are in accordance with previously
reported data.12
1
1-(Allyloxy)-2,4-dimethoxybenzene. Yellow oil, H-NMR (500 MHz, CDCl3) !: 3.76 (3H, s), 3.84 (3H,
s), 4.54 (2H, dd, J = 1.7, 4.6 Hz), 5.26-5.39 (2H, m), 6.07 (1H, m), 6.36 (1H, dd, J = 2.3, 9.2 Hz), 6.50
13
(1H, d, J = 2.3 Hz), 6.81 (1H, d, J = 9.2 Hz). C-NMR (125 MHz, CDCl3) !: 55.7, 55.9, 70.9, 100.5,
103.0, 114.8, 117.9, 133.8, 142.3, 150.6, 154.7. Its spectral data are in accordance with previously
reported data.23
The corresponding allyloxybenzenes were heated at 200 °C for 1 h under N2 to give 2-allylphenol
derivatives.
1
2-Allyl-3,6-dimethoxyphenol. Yellow oil, H-NMR (500 MHz, CDCl3) !: 3.34 (2H, d, J = 6.3 Hz), 3.77
(3H, s), 3.84 (3H, s), 4.95-5.04 (2H, m), 5.71 (1H, s), 5.99 (1H, m), 6.35 (1H, d, J = 8.6 Hz), 6.67 (1H, d,
13
J = 8.6 Hz). C-NMR (125 MHz, CDCl3) !: 27.6, 56.1, 56.5, 101.1, 108.2, 114.4, 115.0, 136.5, 141.2,
144.4, 152.6. Its spectral data are in accordance with previously reported data.12
1
2-Allyl-4,6-dimethoxyphenol. Yellow oil, H-NMR (500 MHz, CDCl3) !: 3.37 (2H, d, J = 6.9 Hz), 3.75
(3H, s), 3.85 (3H, s), 5.04-5.11 (2H, m), 5.30 (1H, s), 5.99 (1H, m), 6.28 (1H, d, J = 2.9 Hz), 6.37 (1H, d,
13
J = 2.9 Hz). C-NMR (125 MHz, CDCl3) !: 34.2, 55.8, 56.1, 97.3, 105.5, 115.7, 125.7, 136.6, 137.5,
147.0, 153.0. Its spectral data are in accordance with previously reported data.24
The corresponding phenols and allyl bromide were refluxed in the presence of K2CO3 for 20 h to give
1-allyl-2-allyloybenzene derivatives.
1
2-Allyl-3-(allyloxy)-1,4-dimethoxybenzene. Yellow oil, H-NMR (500 MHz, CDCl3) !: 3.42-3.44 (2H,
m), 3.77 (3H, s), 3.80 (3H, s), 4.47-4.48 (2H, m), 4.92-4.96 (2H, m), 5.21 (1H, m), 5.38 (1H, m), 6.00
(1H, m), 6.10 (1H, m), 6.56 (1H, d, J = 9.2 Hz), 6.73 (1H, d, J = 9.2 Hz). 13C-NMR (125 MHz, CDCl3) !:
28.5, 56.1, 56.3, 74.0, 105.7, 110.2, 114.5, 117.1, 123.3, 134.5, 137.1, 146.9, 147.4, 152.3. Its spectral
data are in accordance with previously reported data.12
1
1-Allyl-2-(allyloxy)-3,5-dimethoxybenzene. Yellow oil, H-NMR (500 MHz, CDCl3) !: 3.39 (2H, dt, J =
1.7, 6.9 Hz), 3.76 (3H, s), 3.81 (3H, s), 4.40 (2H, dt, J = 1.7, 6.9 Hz), 5.03-5.09 (2H, m), 5.19-5.37 (2H,
m), 5.94 (1H, m), 6.08 (1H, m), 6.28 (1H, d, J = 2.9 Hz), 6.36 (1H, d, J = 2.9 Hz). 13C-NMR (125 MHz,
CDCl3) !: 34.6, 55.6, 55.8, 74.1, 98.3, 105.0, 115.8, 117.2, 134.3, 134.6, 137.2, 139.9, 153.5, 156.1. Its
spectral data are in accordance with previously reported data.12
1-Allyl-2-allyloybenzenederivatives and RuClH(CO)(PPh3)3 (5% mol) were heated in degassed toluene
for 22 h to give aryl enol ethers. These compounds were not characterized further and were used directly

in the next reaction. The aryl enol ethers and the second-generation Grubbs catalyst (5% mol) were
refluxed for 20h under N2 to afford dimethoxybenzofurans.
1
4,7-Dimethoxybenzofuran (3g). Yellow oil, H-NMR (500 MHz, CDCl3) !: 3.89 (3H, s), 3.97 (3H, s),
6.53 (1H, d, J = 8.6 Hz), 6.70 (1H, d, J = 8.6 Hz), 6.86 (1H, d, J = 2.3 Hz), 7.55 (1H, d, J = 2.3 Hz).
13C-NMR (500 MHz, CDCl3) !: 55.9, 56.6, 102.8, 104.5, 106.5, 119.5, 140.5, 144.0, 145.3, 147.7.
EI-LRMS m/z: 178, EI-HRMS m/z: 178.0627 (Calcd for C10H10O3: 178.0630). Its physical and spectral
data are in accordance with previously reported data.12
5,7-Dimethoxybenzofuran (3h). Colorless oil, 1H-NMR (500 MHz, CDCl3) !: 3.83 (3H, s), 3.97 (3H, s),
6.45 (1H, d, J = 2.3 Hz), 6.63 (1H, d, J = 2.3 Hz), 6.69 (1H, d, J = 2.3 Hz), 7.58 (1H, d, J = 2.3 Hz).
13C-NMR (125 MHz, CDCl3) !: 55.9, 56.1, 94.6, 97.1, 107.2, 128.8, 139.7, 145.5, 145.8, 156.9. EI-LRMS
m/z: 178 (M+). EI-HRMS m/z: 178.0630 (Calcd for C10H10O3: 178.0630). (M+). Its spectral data are in
accordance with previously reported data.12
Assay of inhibition
Assays of inhibition of CYP2A6 activity (GENTEST Co. Human CYP2A6 + Cytochrome b5 + P450
reductase (Baculovirus)) by benzofuran and coumarin derivatives were based on microsomal coumarin
7-hydroxylation. The 7-hydroxylation of coumarin was used as the index of CYP2A6 activity. The rate
of coumarin 7-hydroxylation was determined by the method of Yamazaki et al.14 Expressed human
CYP2A6 (0.5 pmol) was incubated with coumarin (final concentration of 1 µM) and an inhibitor
(0.01-10 µM) at 37 °C in the presence of phosphate buffer (10 0 mM, pH 7.4), NADP+ (0.8 mM),
glucose-6-phosphate (7.9 mM), and glucose-6-phosphate dehydrogenase (1 unit/mL) in a total volume of
400 µL. The reaction mixture was preincubated for 2 min at 37 °C, and the reaction was started by the
addition of an NADPH-generating system of the microsomal solution. The reaction was stopped after
6-min incubation with 50 µL of 60% HClO4. After further mixing, the reaction mixture was centrifuged
at 10,000 rpm for 5 min at 4 °C. The supernatant was analyzed by the HPLC-fluorescence method (Ex
338 nm, Em 458 nm). The HPLC system consisted of a Hitachi model L7100 pump, Hitachi D-7500
detector, and Hitachi F-1050 fluorescence spectrophotometer.
Assays of inhibition of CYP3A4 activity (GENTEST Co. Human CYP3A4 + cytochrome b5 + P450
reductase (Baculovirus)) by benzofuran and coumarin derivatives were based on microsomal testosterone
6#-hydroxylation. The rate of testosterone 6#-hydroxylation was determined by the method of Guo et
al.16 Expressed human CYP3A4 (2.5 pmol) was incubated with testosterone (final concentration of 0.2
mM) and an inhibitor (0.01-10 µM) at 37 °C in the presence of phosphate buffer (100 mM, pH 7.4),
NADP+ (0.8 mM), glucose-6-phosphate (7.9 mM), and glucose-6-phosphate dehydrogenase (1 unit/mL)
in a total volume of 500 µL. The reaction mixture was preincubated for 2 min at 37 °C, and the reaction
was started by the addition of an NADPH-generating system of the microsomal solution. The reaction

was stopped after 15-min incubation with 3 mL of AcOEt. After 11!-hydroxy progesterone (internal
standard) had been added, the reaction mixture was centrifuged for 5 min at 2,800 rpm. The supernatant
was analyzed by the HPLC-UV method (240 nm). The HPLC system consisted of a Shimadzu model
LC-10ATvp pump, Shimadzu C-R8A detector, and Shimadzu SPD-10Avp UV spectrophotometer.
ACKNOWLEGEMENTS
This study was partially supported by the Hikari Foundation for Medicinal Research (to KO).
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