Fast Cleavage and Deprotection of Oligonucleotides

Article abstract of DOI:10.1016/S0040-4039(00)73341-7

We have developed methylamine/ammonia as a fast cleavage and deprotection reagent which effects complete cleavage of oligonucleotides from the solid support in 5 min at room temperature and complete deprotection in 5 min at 65 deg C.The problem of transamination side products formation, faced with the commonly used dC^bz (10percent side product) upon treatment with methylamine/ammonia, has been sucessfully solved by the use of dC^ac (0.0percent side product).DMT dC^ac phosphoramidite-methylamine/ammonia system furnished oligonucleotides in equal or superior quality as compared to the other systems.