Synergistic effect of 5-nitro-2'-deoxyuridine with ganciclovir against human cytomegalovirus in vitro

Article abstract of DOI:10.1080/15257779508010719

In this paper we describe a practical synthesis of 5-nitro-2'- deoxyuridine (4) and 1-(2-deoxy-2-fluoro-β-D-arabinofuranosyl)-5-nitrouracil (11). These compounds were then evaluated for their ability to inhibit the growth of human cytomegalovirus (HCMV, strain AD169) in MRC-5 cells using a plaque reduction assay. Compound 11 was unable to inhibit the growth of HCMV at the highest concentration tested (100 μg/mL). However, compound 4 (5- NO₂-dU) exhibited marginal activity against HCMV in vitro in a dose- dependent manner with a 50% inhibitory concentrations (IC₅₀) of 1 to 5 μg/mL. Combinations of 5-NO₂-dU with ganciclovir synergistically inhibited HCMV induced cell killing in culture.

Full text of DOI:10.1080/15257779508010719

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Synergistic Effect of 5-Nitro-2′-
deoxyuridine with Ganciclovir Against
Human Cytomegalovirus In Vitro
T. Sudhakar Rao a , Robert F. Rando a , John H. Huffman b &
Ganapathi R. Revankar a
a Triplex Pharmaceutical Corporation , 9391 Grogans Mill Road,
The Woodlands, TX, 77380
b Institute for Antiviral Research, Utah State University , Logan,
UT, 84322
Published online: 24 Sep 2006.
To cite this article: T. Sudhakar Rao , Robert F. Rando , John H. Huffman & Ganapathi
R. Revankar (1995) Synergistic Effect of 5-Nitro-2′-deoxyuridine with Ganciclovir Against
Human Cytomegalovirus In Vitro , Nucleosides and Nucleotides, 14:9-10, 1997-2008, DOI:
10.1080/15257779508010719
To link to this article: http://dx.doi.org/10.1080/15257779508010719
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