Synthesis of modified oligonucleotides and production of duplexes with covalently linked chains

Article abstract of DOI:10.1080/07328319608002138

A method has been devised to synthesize a DNA-duplex with covalently connected strands. Primary amino group located on one strand is linked to a carboxyl group of the other strand through the agency of a water soluble carbodiimide condensing agent. Conditions for the reaction between chains of a duplex composed of the modified oligonucleotides [1] were optimized. The thermal and hydrolytic stability of the cross linked duplex was examined.

Full text of DOI:10.1080/07328319608002138

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Nucleosides and Nucleotides
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Synthesis of Modified Oligonucleotides
and Production of Duplexes With
Covalently Linked Chains
S. I. Antsypovich a , T. S. Oretskaya a , E. M. Volkov a , E.
A. Romanova a , V. N. Tashlitsky a , M. Blumenfeld b & Z. A.
Shabarova a
a Chemical Department, Moscow State University, Moscow,
119899, Russia
b GENSET, 1 Rue R. et S. Delaunay, Paris, 75011, France
Available online: 20 Aug 2006
To cite this article: S. I. Antsypovich, T. S. Oretskaya, E. M. Volkov, E. A. Romanova, V. N.
Tashlitsky, M. Blumenfeld & Z. A. Shabarova (1996): Synthesis of Modified Oligonucleotides
and Production of Duplexes With Covalently Linked Chains, Nucleosides and Nucleotides, 15:4,
923-936
To link to this article: http://dx.doi.org/10.1080/07328319608002138
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