24β-ETHYLSTEROL BIOSYNTHESIS: INCORPORATION OF L-METHIONINE IN TISSUE CULTURES OF TRICHOSANTHES KIRILOWII

Article abstract of DOI:10.1016/S0031-9422(00)97542-3

A maximum of five deuterium atoms was incorporated into the 24β-ethylsterols, 22-dihydro-25-dehydrochondrillasterol and 22-dihydrochondrillasterol, in tissue cultures of trichisanthes kirilowii incubated with L-methionine.The results proved that a 24-ethylidene intermediate is not involved in the biosynthesis of 24β-ethylsterols in T.kirilowii. Key Word Index: Trichosanthes kirilowii; Cucurbitaceae; tissue culture; biosynthesis, phytosterols; 22-dihydrochondrillasterol; 22-dihydro-25-dehydrochondrillasterol; methionine.