Photolabile linker for the solid-phase synthesis of base-sensitive oligonucleotides

Article abstract of DOI:10.1016/S0040-4039(97)01154-4

o-Nitrophenyl-1,3-propanediol was covalently linked to long chain alkyl amine controlled pore glass (LCAA-CPG) beads through a stable phosphoramidate diester link. The resulting solid support was found suitable for the automated synthesis of 3'-phosphate unmodified as well as base-sensitive oligonucleotides. Efficient cleavage from the solid support was achieved by photolysis.