Imidazole-4-carboxamide and 1,2,4-triazole-3-carboxamide deoxynucleotides as simplified DNA building blocks with ambiguous pairing capacity

Article abstract of DOI:10.1080/07328319808004740

2'-Deoxynucleosides of imidazole-4 (or 1,2,4-triazole-3)-carboxamide, ethyl imidazole-4 (or 1,2,4-triazole-3)-carboxylate were synthesized by enzymatic glycosylation using N-deoxyribosyltransferase from a lactobacterium. The base pairing properties of Y and V when placed opposite the natural DNA bases as well as their serf were evaluated by thermal denaturation experiments. DNA templates containing imidazole-4-carboxamide base were used in elongation reaction catalysed by Klenow fragment.

Full text of DOI:10.1080/07328319808004740

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Imidazole-4-Carboxamide and
1,2,4-Triazole-3-Carboxamide
Deoxynucleotides as Simplified DNA
Building Blocks with Ambiguous Pairing
Capacity
Sylvie Pochet a & Laurence Dugué a
a Institut Pasteur, Unité de Chimie Organique , 28, rue du Docteur
Roux, 75724, Paris, cedex, France
Published online: 22 Aug 2006.
To cite this article: Sylvie Pochet & Laurence Dugué (1998) Imidazole-4-Carboxamide and 1,2,4-
Triazole-3-Carboxamide Deoxynucleotides as Simplified DNA Building Blocks with Ambiguous Pairing
Capacity, Nucleosides and Nucleotides, 17:9-11, 2003-2009, DOI: 10.1080/07328319808004740
To link to this article: http://dx.doi.org/10.1080/07328319808004740
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