Design and synthesis of reagents for phage display screening of dehalogenases

Article abstract of DOI:10.1016/S0960-894X(98)00697-0

Bifunctional molecules containing both a biotin and a substrate unit have been designed and synthesized for phage display screening of mutant libraries of haloalkane enzymes. The molecules were assembled using a convergent modular synthetic strategy. One molecule was synthesized to evaluate the concept of covalent capture and a second for screening of phage libraries for enantioselectivity.