Advanced Synthesis and Catalysis p. 1804 - 1810 (2005)
Update date:2022-08-05
Topics:
Lopez-Gallego, Fernando
Batencor, Lorena
Hidalgo, Aurelio
Mateo, Cesar
Fernandez-Lafuente, Roberto
Guisan, Jose M.
The main drawback in the production of 7-aminocephalosporanic acid (7-ACA) at the industrial level is the inactivation of the enzymes implicated in the process due to the presence of hydrogen peroxide during the reaction. As an alternative, we have developed the conversion of cephalosporin C to 7-ACA in a single reactor without the presence of hydrogen peroxide during the reaction, achieving more than 80% yield. In order to develop this process, D-amino acid oxidase (DAAO) was co-immobilized with catalase (CAT), which is able to fully eliminate in situ the hydrogen peroxide formed by the neighbouring DAAO molecules. Thus, the product of the reaction is only α-ketoadipyl-7-ACA. This system prevents the inactivation of the oxidase by hydrogen peroxide, solving the main problem of the enzymatic process. Moreover, we have found that α-ketoadipyl-7-ACA is recognized as a substrate by glutaryl acylase (GAC) and hydrolyzed as long as glutaric acid is absent from the reaction medium (because it is able to inhibit the hydrolysis). The low stability of α-ketoadipyl-7-ACA justifies the use of a single reactor, in which glutaryl acylase is already present when this substrate is generated. Thus, the whole process may (and must) be performed in a single step, and in the absence of hydrogen peroxide that could affect the stabilities of the involved enzymes.
View MoreContact:+86-371-86058576
Address:NO.32, Jingsan Road, Zhengzhou, China
Luoyang Aoda chemical Co.,Ltd.
Contact:+86-379-67518785 67516588
Address:MiaoWan industry district,YanShi City,Henan,China
Tangshan Moneide Trading Co., Ltd.
Contact:+86-315-8309571
Address:2-7-420 Jidong Building Materials Commercial Center, Tangshan, Hebei, 064000 China
Zhejiang Kaili Industrial Co., Ltd
Contact:+86-571-85241926
Address:lantian business center,No.18 Moganshan Road
Shanghai Forever Synthesis Co.,Ltd.
Contact:021-61124658
Address:Zhoukang Road,Pudong New District,Shanghai,China
Doi:10.1016/S0020-1693(01)00504-7
(2001)Doi:10.1002/jhet.5570370203
(2000)Doi:10.1039/b002413i
(2000)Doi:10.1021/jm00298a046
(1970)Doi:10.1021/ja01045a032
(1969)Doi:10.1016/j.tet.2017.02.061
(2017)