OPTIMIZATION OF THE ENZYMATIC SYNTHESIS OF AMINO ACID ESTERS. REACTION IN POLYPHASIC MEDIUM

Article abstract of DOI:10.1016/S0040-4020(01)91818-6

N-acetyl-L-Tyrosine Ethyl Esters (ATEE) was synthesized from N-acetyl-L-Tyrosine and ethanol, with immobilized chymotrypsin as catalyst.For this purpose, a biphasic liquid reaction mixture consisting of an organic chloroform phase and aqueous phase was used.This system allows the shift of the reaction equilibrium towards ATEE synthesis by achieving continuous ATEE extraction in the organic phase.Synthesis rates of up to 90percent were obtained by optimizing the reaction conditions and controlling the pH of aqueous phase.