Aspartase-Catalyzed Preparative Scale Synthesis of 15N-Aspartic Acid

DOI: 10.1002/jccs.199800078

Source and publish data:

Journal of the Chinese Chemical Society p. 525 - 528 (1998)

Update date:2022-08-04

Topics:

Authors:

Sookkheo, Boonyaras

Phutakul, Suree

Chen, Shui-Tein

Wang, Kung-Tsung

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Article abstract of DOI:10.1002/jccs.199800078

A preparative scale synthesis of ¹⁵N-aspartic acid from fumarate and ¹⁵NH₄OH catalyzed by immobilized aspartase has been developed. Immobilization of aspartase in membrane enclosed enzyme immobilization (MEEI) facilitates the separation of the enzyme from product and makes the enzyme stable and durable for multiple usage. A simple isolation procedure in total yield of 62% using perfusion ion exchange chromatography renders the procedure more practical.

Full text of DOI:10.1002/jccs.199800078

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