Journal of the Chinese Chemical Society p. 525 - 528 (1998)
Update date:2022-08-04
Topics:
Sookkheo, Boonyaras
Phutakul, Suree
Chen, Shui-Tein
Wang, Kung-Tsung
A preparative scale synthesis of 15N-aspartic acid from fumarate and 15NH4OH catalyzed by immobilized aspartase has been developed. Immobilization of aspartase in membrane enclosed enzyme immobilization (MEEI) facilitates the separation of the enzyme from product and makes the enzyme stable and durable for multiple usage. A simple isolation procedure in total yield of 62% using perfusion ion exchange chromatography renders the procedure more practical.
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