Ion-pair extraction of thiocyanate from plasma and its gas chromatographic determination using on-column alkylation

Article abstract of DOI:10.1021/ac00273a036

Tributylsulfonium thiocyanate may be extracted from aqueous solutions with organic solvents. Injection into the heated injection port of a gas chromatograph results in conversion to the volatile derivative butyl thiocyanate. Tributylsulfonium selenocyanate undergoes analogous extraction and thermal conversion to the butyl derivative. A procedure has been developed for the extraction of thiocyanate from human plasma and gas chromatographic determination using selenocyanate as an internal standard. The method has good sensitivity and precision and has been applied to behavioral and pharmacologic studies of smokers.