Indium- and Zinc-Mediated Acyloxyallylation of Protected and Unprotected Aldotetroses - Revealing a Pronounced Diastereodivergence and a Fundamental Difference in the Performance of the Mediating Metal

Article abstract of DOI:10.1021/acs.joc.7b03063

The acyloxyallylation of unprotected aldoses was first demonstrated more than a decade ago as a potentially elegant two-carbon homologation of reducing sugars (upon ozonolysis); however, its application in real case syntheses remained scarce. Following up on such a successful showcase and to answer several pending questions about this attractive transformation, we engaged in an in depth methodological reinvestigation. The epimeric tetroses l-erythrose and d-threose in unprotected and protected form were successfully applied to the indium and also zinc-mediated acyloxyallylation, with the latter being a first for an unprotected sugar. The investigation largely benefited from the choice of these more exotic starting materials as it allowed unambiguous identification/quantification of the hexose-products which are available as authentic reference materials. The observed diastereoselectivities indicate a strong substrate control (stereochemistry at O2), and the influence of the reagent's structure on the selectivity was investigated in great detail. A strong facial diastereodivergence between related protected and unprotected structures was demonstrated and an unexpected, pronounced principle difference in performance between indium and zinc was revealed.

Full text of DOI:10.1021/acs.joc.7b03063

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Article
The Indium and Zinc Mediated Acyloxyallylation of Protected and
Unprotected Aldotetroses - Revealing a Pronounced Diastereodivergence
and a Fundamental Difference in the Performance of the Mediating Metal
Markus Draskovits, Christian Stanetty, Ian R. Baxendale, and Marko D. Mihovilovic
J. Org. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.joc.7b03063 • Publication Date (Web): 25 Jan 2018
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Indium and Zinc mediated Acyloxyallylation of Aldotetroses
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The Indium and Zinc Mediated Acyloxyallylation of Protected and
Unprotected Aldotetroses – Revealing a Pronounced Diastereodivergence
and a Fundamental Difference in the Performance of the Mediating Metal
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Markus Draskovits1, Christian Stanetty1,*, Ian R. Baxendale2, Marko D. Mihovilovic1
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1Institute of Applied Synthetic Chemistry, TU Wien, Getreidemarkt 9, 1060 Vienna, Austria
2Department of Chemistry, Durham University, South Road, Durham, DH1 3LE, United
Kingdom
*E-mail: christian.stanetty@tuwien.ac.at
Dedicated to the memory of Prof. Walther Schmid
Abstract
The acyloxyallylation of unprotected aldoses was first demonstrated more than a decade ago as a
potentially elegant two-carbon homologation of reducing sugars (upon ozonolysis), however, its
application in real case syntheses remained scarce. Following up on such a successful show-case and
to answer several pending questions about this attractive transformation, we engaged in an in depth
methodological re-investigation. The epimeric tetroses L-erythrose and D-threose in unprotected and
protected form were successfully applied to the indium and also zinc mediated acyloxyallylation, the
latter being a first for an unprotected sugar. The investigation largely benefited from the choice of
these more exotic starting materials as it allowed unambiguous identification/quantification of the
hexose-products which are available as authentic reference materials.
The observed diastereoselectivities indicate a strong substrate control (stereochemistry at O2) and
the influence of the reagent’s structure on the selectivity was investigated in great detail. A strong
facial diastereodivergence between related protected and unprotected structures was demonstrated
and an unexpected, pronounced principle difference in performance between indium and zinc was
revealed.
Draskovits, M.; Stanetty, C.*; Baxendale, I. R.; Mihovilovic, M. D.
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Introduction
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Carbohydrates are regularly referred to as an important part of Natures chiral pool. However,
commercial or facile availability at desirably low cost or effort is in fact limited to only selected
representatives of this substance family,1,2 resulting in two undesirable consequences: First, the use
of carbohydrates as chiral starting materials remains often limited to a subset of ’common’ sugars,
and secondly, methodology development is usually only demonstrated on this same set of abundant
derivatives, creating a reinforcing cycle: The use of more exotic sugar building blocks is discouraged
by the lack of positive literature precedence. Approaches to extend the range of sugars are being
targeted by biotechnology3 as well as efforts in the field of de novo syntheses4-6 , however, the
challenge of mastering the stereoselective modification of one or more centers of an otherwise readily
available chiral scaffold is certainly an attractive complementary endeavor. Our current report
outlines the potential value of including currently less abundant sugars into a methodological study
and in parallel serves our ultimate motivation to increasing the platform of readily available parent
sugars for the carbohydrate community and beyond.
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In this context, the indium-mediated acyloxyallylation (IMA) of unprotected aldoses with halopropenyl
esters as reactants presents great potential as an elegant two-carbon homologation of reducing sugars
(upon facile loss of ester protection and subsequent ozonolysis). The use of IMA is a particularly
practical approach as the indium (and analogous zinc) organometallics can be formed and reacted
under Barbier-type-conditions. Of note the observed selectivities have been shown to be independent
of the (E/Z)-configuration of the reagents and a wide range of reaction conditions are tolerated
including protic solvents (alcohols, aqueous solutions) and ambient temperatures.7-10 This inherent
compatibility with unprotected sugars highlights this method from other homologation methods (in
particular by two carbons) including alternative -hydroxyallylations (based on Li, B, Ti, Al, Cr, Zr)
which generally require low temperatures and/or anhydrous conditions.7,11,12 In an initial proof of
concept study, standard D-pentoses and D-hexoses were studied as starting materials and the
formation of only two out of four possible isomers was reported, with moderate to good selectivity
for the main product.13 Only the main products were isolated and identified which exhibited
consistently the same relative stereochemistry, namely a lyxo-configuration. This configuration
represents an anti-orientation of the two new stereocenters formed, in a syn-fashion with respect to
the -position of the carbonyl moiety in the starting material (Figure 1, top). While the anti-addition
was in line with the established model for the acyloxyallylations of achiral aldehydes,9 the facial
selectivity (syn) in respect to the addition to the carbonyl seems specific to sugars as starting materials.
The other isomers were not isolated or identified. Therefore, although widely acknowledged in the
literature this chemistry was never fully explored or exploited.
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Figure 1: Progress of the state of knowledge of the indium (and zinc) mediated acyloxyallylation of aldoses. Reagent (blue)
and sugar-derived (red) new stereocenters and partial structures are color-coded.
Recently, we developed a large-scale concise synthesis of the important bacterial sugar L-glycero-D-
manno heptose 2 starting from L-lyxose, featuring a practical preparative indium mediated
acetoxyallylation protocol towards the highly crystalline manno-configured enitol 1a (lyxo-type
addition) as the key synthetic step.14 En route, we additionally isolated two further isomers which were
identified as the gluco- and allo-configuration (1b, 1c), derived from xylo- and ribo-type addition,
respectively (Figure 1, middle). In contrast to the original paper (D-lyxose)13 we observed a significantly
less pronounced selectivity for the main isomer and also less of an enhancement when replacing
bromopropenyl acetate (1a/1b/1c = 65:25:10) with the corresponding benzoate (1a/1b/1c =
70:20:10). Success in this case study was ultimately derived from the beneficial physical properties of
enitol 1a in its downstream processing. The observed ratios imply a high facial selectivity for the attack
of the indium organyl from the si-face of the carbonyl (90% for the two main products), with a
moderate anti-selectivity (1a/1b ~2:1 up to 3.5:1) in the addition step (see Figure 2 for the separate
consideration of the two types of selectivity). To the best of our knowledge, this constitutes the first
complete set of data describing the outcome of an indium mediated acyloxyallylation in a complex
setting that employing sugars as starting materials constitute. Building upon the knowledge
generated, we have decided to expand our efforts into a methodological study to address several open
questions in this attractive transformation.
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Setting up our methodological survey. To cope with the inherent difficulty of structural analysis of
unknown carbohydrate structures as well as the reliable quantification of (isomeric) mixtures thereof
we chose the tetroses, L-erythrose and D-threose, as substrates for our survey. These species
represent the two different relative stereochemical configurations (erythro, threo) next to the reactive
carbonyl center and with hexoses being the final elongation products (upon ozonolysis), unambiguous
identification of all potential products can be guaranteed by comparison with authentic reference
materials.
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The first question we wished to investigate was whether the product distribution, revealed in our case
study, was general and if the moderate selectivity in the addition step could be improved upon via
optimization of the reagent. Furthermore, according to the reported literature as well as our own
experience, replacement of indium with cheaper zinc was unsuccessful in the acyloxyallylation of
aldoses while it gave comparable results with standard aldehydes, a result not explained so far.8,9 Thus,
we set out better understand the particular challenge of employing zinc in the case of carbohydrate
starting materials (Figure 1, bottom right). We also decided to evaluate the corresponding 2O,3O-
isopropylidene protected derivatives in our survey, expecting to observe facial diastereodivergence,
yielding the anti/anti-products (ribo-type addition) (Figure 1, bottom left). Precedence for such an
inversion of selectivity can be found for example with Garner’s aldehyde, one of the few
transformations of chiral starting materials with bromopropenyl esters.8,15,16 A related facial
diastereodivergence was also reported in the simple allylation (only one new stereocenter) of
protected and unprotected sugar derivatives17,18 and the 1,2-addition of 3-bromomethyl-5H-furan-2-
one to -chiral aldehydes under the mediation of indium.19
Figure 2: Hypothetical separation of the two types of selectivities observed in the acyloxyallylation of chiral substrates with
color-coding for substrate (red) and reagent (blue) derived stereocenters and the path to the observed main product in bold.
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For the sake of clarity, the overall process of the addition is hypothetically separated into the approach
of the indium organyl onto the carbonyl, related to the facial selectivity (carbonyl), and the actual
addition step to the carbonyl, related to the simple diastereoselectivity in the formation of the two
new stereocenters (Figure 2).
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Results and discussion
Acetyloxyallylation of protected and unprotected L-erythrose. We started our investigation with the
erythro-series. We first synthesized L-erythrose (available but at high prices) from cheap L-arabinose
by 3O/4O-isopropylidene protection (4), oxidative cleavage with NaIO4 (5)20 and subsequent acidic
hydrolysis to furnish 6. It is noteworthy that a high degree of the open chain form is present in
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solutions of 6 (~10% as hydrate in according to H-NMR in D2O), which is of importance in the later
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discussion. Alternative treatment of 4 with silica supported NaIO4 under non-aqueous conditions
furnished protected L-erythrose sugar aldehyde 7 species, a more reactive version of 5 (Scheme 1).
Scheme 1: Synthesis of unprotected (6) and protected L-erythrose sugar aldehyde 7
When L-erythrose 6 and bromopropenyl acetate 3a9 were subjected to the standard reaction
conditions as applied in our preliminary case study (EtOH, Indium, 45 °C, 10 min), rapid and full
conversion was observed, delivering a mixture of three isomeric enitols (8a/8b/8c = 66:18:14) upon
deacetylation. The two major isomers (8a, 8b) were obtained in pure form via their peracetates (9a,
9b) and were subjected to a modern ozonolysis protocol22 adopted by us for polar compounds14
towards the corresponding sugars L-mannose 10 and L-glucose 11 (Scheme 2, middle). Their structures
were unambiguously proven by comparison (1H, 13C NMR) to authentic samples (D-hexoses), thus
confirming the expected additions (lyxo, xylo) as predicted from our case study. The stereochemistry
of the third compound 8c was elucidated to be the allo (anti/anti), upon isolation from the analogous
experiments with sugar aldehyde 7, in which 8c was formed as the major isomer accompanied by the
altro-isomer 8d (8c/8d = 84:12). Ozonolysis of the crude allo-enitol (8c/8d) allowed the isolation of
pure L-allose 12 achieved by recrystallization and identification of both sugars (L-altrose 13 in mother
liquor) again by comparison with authentic samples (Scheme 2, bottom). All attempts to achieve
analogous conversion of 5 (as a simpler version of 7) under several conditions in different solvents
remained unsuccessful which is attributed to the high stability of the bicyclic system in lactol 5
resulting in low formation of the required open chain form. Quantification of isomers 8a-d was
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consistently performed either by H-NMR (diagnostic allylic signals of processed mixtures) or more
conveniently by GC-analysis of crude mixtures upon per-OTMS silylation.23
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Scheme 2: Facial diastereodivergence in the indium mediated addition of 3a to unprotected (6) and protected L-erythrose (7)
In summary a high degree of facial diastereodivergence was observed with 6 and 7, respectively. While
the facial (carbonyl) selectivity was high (85-95%) in both cases, the diastereoselectivity in the addition
step was high (7:1) in the protected case (7) but only moderate for the acyloxyallylation of the
unprotected L-erythrose (5). The ratio found (8a/8b/8c = 66:18:14) in the latter case, is in line with the
results of our previous case study, both with respect to the newly formed stereochemical constitution
in the products (lyxo, xylo, ribo) as well as the ratios between them.
The facial selectivity for the si-face in the case of the unprotected sugars can be rationalized by a Cram-
chelate model while a Cram-type model (with the O2-oxygen in the antiperiplanar position to the
carbonyl) predicts the diastereoselectivity for the protected case (Figure 3, bottom).17 The anti-
selectivity in the addition step to the carbonyl is in line with the mechanistic model established for the
acyloxyallylation of achiral aliphatic aldehydes by Lombardo and Trombini (vide infra).9
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Figure 3: Rationale for the facial selectivity in the acyloxyallylation relating to the dependence of O2/O3-isopropylidene
protection.
Acetyloxyallylation of protected and unprotected D-threose. Next, D-threose 14 (the 3-epimer of L-
erythrose) and the related protected sugar aldehyde 15 were subjected to the same reaction
conditions as indicated above (3a, In, EtOH, 45 °C). Compound 15 was prepared via a literature
approach24 with necessary modification of the final oxidation step.25 Clean and full conversions was
again achieved for both starting materials and the crude reactions mixtures were processed to their
fully unprotected enitols 16a-d for quantification as described above (Scheme 3, top).
Scheme 3: Facial diastereodivergence in the indium mediated addition of 3a to unprotected (14) and protected D-threose (15)
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A very pronounced facial diastereodivergence between the conversion of unprotected (14) and
protected D-threose 15 with a high degree of facial diastereoselectivity (>90%) was observed. In
addition the simple diastereoselectivities were consistent with the erythro-case, both in terms of types
of addition products as well as their ratios (14: lyxo>xylo>ribo; 15: ribo>arabino).26 A comparably
lower selectivity was observed in the conversion of 15 (~7:2 anti:syn in the threo-case versus ~7:1 in
the erythro-case), noteworthy, due to different synthetic routes, O4-acetate protection was in place
in 15 in contrast to the O4-formate in 7. Purified diasteromers 16a-d were obtained via
chromatographic separation of the corresponding peracetates 17a-d or by direct crystallization as in
the separation of the talo- and galacto- configured enitols 17c/17d. The stereochemical constitution
was again proven by conversion to the reducing sugars (18-21) and comparison (1H and 13C-NMR) to
authentic commercial samples.
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To facilitate the comparison of the results of the erythro- and threo-series the corresponding product
distributions are summarized in Figure 4, with reference to the stereochemical-type of addition rather
than the actually formed structures.
Figure 4: Summary of product-distributions in the acyloxylallylations of protected and unprotected tetroses (6, 7, 14, 15). The
formed products are referred to as by the newly established relative stereochemistry (xylo, lyxo, ribo, arabino) to allow for
comparison between the two series.
Optimization of the reagent to improve simple diastereoselectivity. Prompted by the moderate
selectivities in the conversion of the unprotected tetroses (6, 14), we decided to systematically
investigate if the steric bulk of the ester moiety in the reagent can positively modulate the simple
diastereoselectivity observed. Our hypothesis was based on the mechanistic model established for
simple achiral aldehydes by Lombardo and Trombini that aldoses apparently follow as well (Figure 5).
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The model rationalized the observed anti-selectivity with saturated aldehydes (and the stereo-
crossover with unsaturated aldehydes exhibiting syn-selectivity)9,27 with a boat-like conformation in
the relevant transition states (TS) which was further supported by an extensive computational study.28
The boat-like conformation allows an additional positive stabilization of the metal center by the ester
moiety (as in the y-Z-form of the parent indium organyl) which is not in place in the alternative classical
Zimmerman-Traxler model. The TS leading to syn or anti-addition products according to the suggested
model (top) and the alternative classical Zimmerman-Traxler model, predicting generally syn-addition,
are depicted for the erythro-products 6 and 7 in Figure 5. Instead of showing an enantiomeric TS for
7, enantiomeric ent-7 is depicted to correctly represent the formation of the two different major anti-
products.
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Figure 5: Mechanistic rational for the expected increase of simple diastereoselectivity with increasing bulk of the ester group
due to steric clash with the sugar chain of 6 and 7 as examples. Instead of depicting the enantiomeric ent-TS A for the reaction
with acetonide 7, its enantiomer ent-7 is depicted to account for the opposite facial selectivity correctly.
According to the Lombardo-Trombini-model, the formation of an anti-isomer should be independent
of the size of the ester moiety (TS-A) while the transition states leading to the syn-isomer (TS-B but
even TS-D) would be effected by the steric congestion between the residue (sugar chain) and the ester
group. Therefore, increasing bulk in the reactant would be expected to favor formation of the
preferred anti-isomer (i.e. 8a, 8c).
To test this hypothesis we synthesized a series of bromopropenyl esters with different steric demand
(isobutyrate 3c, pivalate 3d, benzoate 3b, 1-naphtoate 3e and mesitoate 3f)27 and utilized them in the
acyloxyallylation of L-erythrose 6. Iodopropenyl pivalate 22 (via the corresponding chloride 2328) was
included in the survey as a particularly reactive species assist the replacement of indium by zinc as the
mediating metal. Noteworthy, in all transformations of 3a-f with indium a full and clean conversion of
6 was achieved. Upon deacylation and subsequent GC-analysis a stepwise increase of the simple
diastereoselectivity (8a/8b-ratio) was observed (3a < 3c < 3d, 3b) (see Figure 6, lines 1-4). The
application of the corresponding naphthoate 3e did not give a significant additional increase in
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selectivity compared to benzoate 3b (line 4-5) and an attempted investigation of mesitoate 3f29
(structure not shown) was hindered as, upon successful acyloxyallylation, the ester could not be
cleaved to allow isomer-analysis. Of note the difference in selectivity between 3a and 3d is significantly
more pronounced compared to our case study with L-lyxose, a pentose with the same stereochemistry
at O2/O3 compared to 6.14
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Figure 6: Product ratios of the acyloxyallylation of L-erythroses 6 and 7 depending on the used reagent and metal.
Realization of the zinc mediated acyloxyallylation of an unprotected sugar. The reaction of iodide 22
gave comparable product ratios to the standard bromide 3d as mediated by indium in addition it also
showed complete product formation when reacted with zinc dust. This is in strong contrast to all
earlier reports including our own experience with L-lyxose which failed to give any measurable
conversion. To our best knowledge, this constitutes the first successful zinc-mediated acyloxyallylation
of an unprotected aldose. However, closer analysis of the enitol product distribution revealed an
entirely different picture compared to the use of indium. The observed product ratios
(8a/8b/8c/8d = 38:9:40) indicated that the facial selectivity was completely lost while the simple
diastereoselectivity remained in a similar range. The loss of facial selectivity under mediation of zinc
was confirmed with other reagents (3a shown), revealing a striking different in principle performance
between indium and zinc in the acyloxyallylation of a chiral chelating starting material like L-erythrose
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(Figure 6, lines 6-8). In contrast, the analogous experiment with acetonide 7, 3a and zinc showed no
detrimental effect on the facial-selectivity providing comparable values to the alternative indium
mediation (Figure 6, lines 9-10). For the latter case the study with chiral Garner’s aldehyde can be
considered precedece.9
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Mechanistic considerations for the zinc-mediated acyloxyallylation. Although, the lost facial
selectivity with zinc as mediator renders the replacement of indium synthetically irrelevant, it enables
some interesting deductions to be elicited. The striking difference between the indium and zinc
organyls supports an interpretation that the facial syn-selectivity observed in the unprotected case
with indium (but not zinc) results from a chelating effect of the incoming organyl. The alternative
explanation within the framework of a Cram-model would require the sugar chain of 5 to adopt an
antiperiplanar orientation to the carbonyl (instead of the O2 with 7, Figure 3) but this would not
explain the complete loss of facial selectivity when zinc is used. Nonetheless, the sole reactivity of L-
erythrose 6 under zinc mediated reaction is quite remarkable and we hypothesize this originates from
the exceptionally high proportion of open chain form in tetroses (~10%) which would single them out
from the all other longer-chain parent aldoses. The high concentration of available aldehyde species
(and more importantly, the fast re-equilibration to form this species) allows the acyloxyallylation
under the mediation of zinc to take place rapidly. With the usual pentoses/hexoses, the (re)formation
of the open chain structure from the dominant cyclic hemiacetal is apparently too slow to compete
with reagent side reactions (e.g. Wurtz type dimerization, alcoholysis).
Elucidation of the fate of halopropenylesters in alcoholic solutions. Only a short reaction time is
required for complete and clean conversion in all indium mediated acyloxyallylations with
unprotected sugars, but requiring an excess of reagent/indium and importantly sufficient stirring.30
The fast formation of Wurtz-type byproducts in water has been reported9 as a potential sink of reagent
3a, which could not be confirmed by us in EtOH in blank experiments; only small amounts of material
(potential Wurtz-type products) were recovered with volatiles being the major side-products. In order
to increase the understanding of the fate of the bromopropenyl esters under the protic conditions
required to solubilize unprotected sugars we dissolved 3a in MeOH-d4 and observed its rapid
alcoholysis (to 25-d6) and slower conversion to the 26-d10 (deuterated version of 1,1,3-tri-methoxy
propane 26) over time (1H-NMR). The formation of 26-d10 was supported by analogous reactions in
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MeOH and EtOH at preparative scale and comparison of isolates to commercial 26 and 27 (Scheme 4
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and supporting information).
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Scheme 4: Fate of bromopropenylacetate 3a in alcoholic solutions (at NMR and preparative scale)
From this investigation we conclude that rapid alcoholysis consumes the reagent in competition with
the formation of the indium organyl, and in doing so liberates HBr which is responsible for the
observed drop in pH and likely also for the formation of ethyl glycosides, observed in the case of
incomplete initial conversion.13,14 Under ideal reaction conditions, the formation of the indium organyl
is fast enough to give a clean conversion even with standard reducing sugars (low content of open
chain form). As indicated by the full conversion of 6, the analogous zinc organyls can apparently be
formed under the same reaction conditions, but more readily available aldehyde species are required
to achieve acyloxyallylation (in time). This prerequisite is fulfilled in the case of L-erythrose but not
with the other tested aldoses. Whether under the indium mediation, the chelation with O2 is not only
responsible for the high degree of facial selectivity but also activates the reducing sugar towards the
reaction, cannot currently be answered.
Conclusion. With a coherent set of molecular probes (tetroses) we have undertaken an in-depth
investigation of the indium and zinc mediated acyloxyallylation of protected and unprotected tetrose
structures. It has been shown that independent of the relative stereochemistry at C2/C3, consistent
product distributions (also to the one described by us earlier for L-lyxose) can be attained. A generally
high facial selectivity is exhibited, outlining a strong substrate control with respect to the
stereochemistry in the -position to the reactive carbonyl. Furthermore, a pronounced
diastereodivergence was observed depending on whether or not O2/O3-isopropylidene protection
was in place. Increasing bulk in the ester group promotes the selectivity for the lyxo-type addition to
L-erythrose, an observation which is consistent with the model established for achiral aldehydes. With
unprotected L-erythrose, we accomplished for first time, the replacement of indium by zinc as the
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acyloxyallylation mediator however the high facial selectivity observed with indium was entirely lost,
a striking difference between the two metals which can only be observed in this complex setting of a
chelating chiral starting material. This observation is a beautiful example of the value of including
other more exotic sugars into methodological work. Through a structured and detailed investigation
of the indium and zinc mediated acyloxyallylation of tetroses several outstanding questions pertaining
to this attractive but complex transformation have finally been clarified. This consolidated knowledge
allows for more refined predictions and we hope will inspire more people to consider acyloxyallylation
as synthetic tool, within and beyond the realm of carbohydrate chemistry. Applications towards
further short synthetic routes to currently rare and exotic sugars based on acyloxyallylation are
currently being developed in our lab.
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Experimental Section
General Methods.
All starting materials and reagents as well as the reference materials for D-Hexoses (Carbosynth, UK)
were purchased from commercial sources and used without further purification. Dowex® 50WX2
hydrogen form was used as an acidic ion exchange resin. Reactions were monitored by TLC on silica
gel 60 F254 plates; spots were detected by UV light examination or visualized by spraying with
anisaldehyde−sulfuric acid and heating. Normal-phase column chromatography was performed on
silica gel 60 (230−400 mesh). NMR spectra were recorded at 297 K in the solvent indicated, with 400
and 600 MHz instruments, respectively, employing standard software provided by the manufacturer.
1H-NMR and 13C NMR spectra were referenced to tetramethylsilane (TMS, δ = 0) by calibration with
the residual organic solvent signals.31 All assignments are based on COSY, HSQC, and HMBC
experiments. Accurate mass analysis (2 ppm mass accuracy) was carried out from 10−100 mg/L
solutions via LC−TOFMS measurements using an autosampler, an HPLC system with binary pumps,
degasser, and column thermostat and ESI-TOF mass spectrometer. Optical rotation was determined
from solution of the indicated solvent and measured on an Anton Paar MCP 300 circlepolarimeter.
The used cuvette was a 100 mm-cell with serial number: 16037274. Melting points were determined
with a Büchi Melting Point B-545 apparatus with a heating rate of 1 °Cmin-1 (70% onset point and 10%
clear point) or on a Kofler Block apparatus. Compounds 3a27, 3b27, 4-520 and 2332 were prepared
according to known literature procedures. Compounds 3c-e, 7 and 22 reproducibly did not give
conclusive HRMS results which was attributed to their labile nature which also prevented purification
to the high purity required for elemental analysis.
Determination of enitol distribution derived from erythro-configured starting materials (GC)
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Synthesis of heptenitoles 8a-d starting from L-erythrose 6 on analytical scale. L-Erythrose 6 (0.1-0.4
mmol) was dissolved in dry EtOH (0.2 M) and heated to 45 °C. Then, the corresponding
halopropenyl ester (3.00 equiv) and indium or zinc (2.00 equiv) were added under vigorous stirring
and in immediate succession. After 30 minutes complete conversion of starting material (staining
yellow/green) to a more apolar spot (staining blue) was obtained according to TLC analysis
(DCM/MeOH 9:1). The reaction mixture was filtered and solvent and volatiles evaporated.33 The white
residue was taken up in MeOH (0.1 M) and NaOMe was added until a basic pH was reached, which led
to the formation of a white precipitate. The reaction mixture was stirred at rt until TLC analysis
(DCM/MeOH 9:1) showed complete conversion to a more polar spot when the white precipitate was
centrifuged and the supernatant was neutralized with Dowex-H+. The resin was filtered and an aliquot
of the filtrate was subjected to GC analysis.
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Synthesis of heptenitoles 8a-d starting from 2/3O-protected erythrose 7. Dry EtOH (0.1 M) was
heated to 45 °C in a round bottom flask. In immediate succession, first indium or zinc (2.00 equiv),
freshly distilled 3-bromoprop-1-en-1-yl acetate 3a (3.00 equiv) and subsequently aldehyde 7 (1.00
equiv, 0.1-0.4 mmol) was added as a solution in little EtOH in one portion. The reaction mixture was
stirred at 45 °C for 30 min, when TLC (LP/EtOAc 1:1) showed complete conversion to a more apolar
product (staining blue; starting material yellow). The reaction mixture was filtered and the filtrate was
evaporated, leaving a white residue, which was acetylated using Ac2O in pyridine, followed by aqueous
workup to remove all inorganics. The acetylated product mixture was taken up in dry MeOH and
treated with NaOMe until a pH of 9-10 was reached. The mixture was stirred at rt for 1 hour, when
reaction monitoring via TLC (LP/EtOAc 1:1 & 1:3) showed complete conversion to a more polar spot.
The reaction mixture was neutralized by addition of Dowex-H+ resin and filtered. Fresh (MeOH
washed) Dowex-H+ was added until a pH< ~2 was determined and the reaction mixture was stirred at
rt for 2 h, when reaction monitoring via TLC (DCM/MeOH 9:1) showed complete conversion to fully
unprotected heptenitoles 8a-d. The resin was filtered and an aliquot was subjected to GC analysis.
Persilylation (OTMS) of crude enitols 8a-d and GC-analysis. An aliquot (containing approx. 0.1-0.2 mg
of enitole species) of crude enitol containing solutions was evaporated to dryness. To the dry residue,
glycerol (silylation standard) was added in ~equimolar amounts. The mixture was taken up in a
solution of DMAP in pyridine (c(DMAP) 0.75 mg/mL; 400 µl). N,O-Bis(trimethylsilyl)trifluoroacetamide
(200 µl, incl. 1% (v/v) TMSCl) was added and the mixture was stirred at 70 °C for 4 h. EtOAc (400 µl)
was added and after filtration through a syringe filter, the samples were analyzed via GC. The ratio of
diastereomers was determined by gas chromatography (GC) using a Thermo Finnigan Focus GC / DSQ
II equipped with a standard capillary column (BGB5, 30 m x 0.25 mm ID, 0.50 μm film) and a FID
detector. carrier gas: helium, injector: 230 °C; column flow: 2.0 mL/min; oven program: 50-190 °C (50
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°C/min)  190-220 °C (3 °C/min)  220-310 °C (50 °C/min)  310 °C (2 min). Retention times: 9.62
(8c), 9.75 (8a), 9.85 (8b), 9.98 (8d) min.
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Determination of enitol distribution derived from threo-configured starting materials (1H-NMR)
Synthesis of heptenitoles 17a-d (peracetates) starting from D-threose 14 on analytical scale. D-
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Threose 14 (0.5 mmol) was dissolved in dry EtOH (0.2 M) and heated to 45 °C and bromopropenyl
acetate 3a (3.00 equiv) and indium (2.00 equiv) were added under vigorous stirring in immediate
succession. After 30 minutes complete conversion of starting material (staining yellow/green) to a
more apolar spot (staining blue) was obtained according to TLC analysis (DCM/MeOH 9:1). The
reaction mixture was filtered and solvent and volatiles evaporated. The white residue was acetylated
with Ac2O in pyridine followed by an acidic aqueous workup to remove all inorganics. The crude
mixture was analyzed by 1H-NMR to determine the enitol ratios by comparison to reference materials
(see supporting information for a comparison of the crude mixture with purified 17a-c).
Synthesis of heptenitoles 16a-d starting from 2/3O-protected D-threose 15. Dry EtOH (0.1 M) was
heated to 45 °C in a round bottom flask. In immediate succession, first indium (2.00 equiv), freshly
distilled 3-bromoprop-1-en-1-yl acetate 3a (3.00 equiv) and subsequently aldehyde 15 (1.00 equiv,
0.1-0.4 mmol) was added as a solution in little EtOH in one portion. The reaction mixture was stirred
at 45 °C for 30 min, when TLC (LP/EtOAc 1:1) showed complete conversion to a more apolar product
(staining blue; starting material yellow). The reaction mixture was filtered and the filtrate was
evaporated, leaving a white residue, which was acetylated with Ac2O in pyridine, followed by aqueous
workup to remove all inorganics. The acetylated product mixture (17a-d) was taken up in dry MeOH
and treated with NaOMe until a pH of 9-10 was reached. The mixture was stirred at rt for 1 hour, when
reaction monitoring via TLC (LP/EtOAc 1:1 & 1:3) showed complete conversion to a more polar spot.
The reaction mixture was neutralized by addition of Dowex-H+ resin and filtered. Fresh (MeOH
washed) Dowex-H+ was added until a pH< ~2 was determined and the reaction mixture was stirred at
rt for 2 h, when TLC-analysis (DCM/MeOH 9:1) indicated complete conversion to the fully unprotected
heptenitoles 16a-d. The resin was filtered, the filtrate was evaporated and passed over a short bed of
SiO2 (DCM/MeOH 4:1) to separate reagent based side products and the crude enitol mixture was
analyzed by 1H-NMR (see supporting information).
General procedure 1 for the ozonolysis of enitoles. Heptenitol (1.00 equiv) was dissolved in 3:2
H2O/acetone (2% (w/v)). A small amount of Sudan red III in acetone (as indicator) was added and the
mixture was cooled with an ice-bath. Ozone was bubbled through the reaction through a gas inlet
tube, the gas outlet was passed through an aq. KI (10 % w/w) solution in a gas wash bottle. As soon as
the bright pink color of the indicator diminished, TLC analysis (CHCl3/MeOH/H2O 14:6:1) was carried
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out to confirm complete conversion of starting material to a more polar, smearing spot. Oxygen was
bubbled through the solution for ~15 min before additional acetone (to solubilize the PPh3) and PPh3
(2.00 equiv) were added and stirring was continued at rt overnight when peroxide tests (test stripes)
indicated complete reduction of all peroxides and H2O2. The reaction mixture was concentrated and
the remaining aqueous layer was washed with DCM, EtOAc and Et2O before it was lyophilized and co-
evaporated from MeOH twice (removing HCHO) to obtain the corresponding hexose.
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3-Bromoprop-1-en-1-yl isobutyrate (3c). Acrolein (95%, 7.14 mL, 107 mmol, 1.00 equiv) was dissolved
in dry DCM (85 mL) and cooled to -20 °C, using an acetone/liquid N2 cooling bath. First, isobutyryl
bromide (9.03 g, 101 mmol, 0.95 equiv) followed by anhydrous ZnCl2 (0.15 g, 1.07 mmol, 0.01 equiv)
was added. The reaction mixture was stirred and allowed to warm by lowering the cooling bath until
-15 °C, when an exothermic reaction caused warming up to +10 °C. The flask was re-immersed in the
cooling bath and the temperature was kept under -10 °C for 1 hour. A sample (micro workup with Et2O
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and aq. NaHCO3 and drying with MgSO4) for analysis via H-NMR was taken, confirming complete
conversion of starting material to the target products. Under cooling H2O (40 mL) was added
(temperature rose to -10 °C), which led to the formation of a white precipitate. Layers were separated
and the organic layer washed with H2O (still acidic) and sat. aq. NaHCO3 (until basic pH). The organic
layer was washed with brine, dried over MgSO4 and the solvent evaporated, leaving a brown liquid
(17.3 g) which was purified by distillation (bp 43 °C, 0.4 mbar) to give pure target compound 3c as
colorless liquid (9.00 g, 41%): ratio of E/Z = 1:1.4; (E)-isomer 1H-NMR (400 MHz, CDCl3) δ 7.44 (dt, J =
12.4, 1.1 Hz, 1H, =CH-O), 5.71 (dt, J = 12.4, 8.4 Hz, 1H, CH2-CH=), 4.00 (dd, J = 8.5, 1.0 Hz, 2H, CH2-Br),
2.63 (hept, J = 7.0 Hz, 1H, CH-(CH3)2), 1.21 (d, J = 7.0 Hz, 6H, CH-(CH3)2); 13C-NMR (101 MHz, CDCl3): δ
= 173.7 (s, C=O), 139.5 (d, O-CH=), 111.1 (d, =CH-CH2), 33.8 (d, CH-(CH3)2), 28.8 (t, -CH2-Br), 18.7 (q, -
CH3) ; (Z)-isomer: 1H-NMR (400 MHz, CDCl3) δ 7.20 (dt, J = 6.3, 0.8 Hz, 2H, =CH-O), 5.25 (td, J = 8.4, 6.3
Hz, 1H, CH2-CH=), 4.08 (dd, J = 8.4, 0.8 Hz, 2H, CH2-Br), 2.70 (hept, J = 7.0 Hz, 1H, CH-(CH3)2), 1.25 (d, J
= 7.0 Hz, 6H, CH-(CH3)2); 13C-NMR (101 MHz, CDCl3) δ 173.3 (s, C=O), 137.5 (d, O-CH=), 109.5 (d, =CH-
CH2), 34.0 (d, CH-(CH3)2), 23.7 (t, -CH2-Br), 18.8 (q, -CH3);
3-Bromoprop-1-en-1-yl pivalate (3d). Step 1 - synthesis of pivaloyl bromide: PPh3 (26.2 g, 0.1 mol, 1.00
equiv) was dissolved in dry DCM (50 mL) and cooled to 0 °C via an ice-bath. Br2 (5.12 mL, 0.1 mol, 1.00
equiv) was added as a solution in dry DCM (50 mL) dropwise, keeping the temperature at 0 °C. PPh3Br2
started to precipitate. After complete addition of Br2, pivalic acid was also dissolved in dry DCM (50
mL) and added quickly to the reaction mixture. The previously formed precipitate got dissolved and
stirring was continued at rt for 1 hour. Solvent was evaporated and the residue was treated with dry
Et2O, which led to the formation of a lot of precipitate, which was filtered and the solvent was
evaporated. The product 3d was obtained by distillation under reduced pressure as a colorless liquid
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(5.3 g, 32%): bp 25 °C, 10 mbar (lit.34 65 °C, 15 Torr); 1H-NMR (400 MHz, CDCl3) δ = 1.30 (s, 1H, 3×CH3);
13C-NMR (101 MHz, CDCl3) δ = 178.8 (COBr), 52.8 (C(CH3)3), 27.2 (3×CH3). Step 2: Acrolein (95%, 2.38
mL, 35.7 mmol, 1.00 equiv) was dissolved in dry DCM (14 mL) and cooled to -20 °C, using an acetone
cooling bath. Then, pivaloyl bromide (5.30 g, 32.1 mmol, 0.95 equiv) was added and subsequently
anhydrous ZnCl2 (40 mg, 0.32 mmol, 0.01 equiv). The reaction mixture was stirred and allowed to
warm to -15 °C, when an exothermic reaction caused warming up to +10 °C. The flask was immersed
in the cooling bath again and the temperature was kept under -10 °C for an hour and a sample (micro
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workup with Et2O and aq. NaHCO3 and drying with MgSO4) for analysis via H-NMR was taken. This
showed complete conversion of starting material to the desired product. H2O (10 mL) was added
under cooling (temperature rise to -10 °C. Layers were separated and the organic layer washed with
H2O (still acidic) and sat. aq. NaHCO3 (until basic pH). Then, the organic layer was washed with brine,
dried over MgSO4 and the solvent evaporated, leaving a brown liquid (9.03 g). The product 3d was
obtained by distillation under reduced pressure as a colorless liquid (3.00 g, 42%): bp 38 °C, 0.2 mbar;
1
ratio of E/Z 1:1.7; (E)-isomer H-NMR (400 MHz, CDCl3) δ 7.42 (dt, J = 12.4, 1.0 Hz, 1H, =CH-O), 5.70
(dt, J = 12.4, 8.5 Hz, 1H, =CH-CH2), 3.99 (dd, J = 8.5, 1.1 Hz, 2H, CH2-Br), 1.23 (s, 9H, 3× CH3); 13C-NMR
(101 MHz, CDCl3) δ 175.2 (C=O), 139.7 (d, O-CH=), 111.1 (d, =CH-CH2), 28.9 (t, CH2-Br), 27.0 (q, 3×
C(CH3)3); (Z)-Isomer 1H-NMR (400 MHz, CDCl3) δ 7.18 (dt, J = 6.2, 0.8 Hz, 1H, =CH-O), 5.25 (td, J = 8.3,
6.2 Hz, 1H, =CH-CH2), 4.07 (dd, J = 8.4, 0.8 Hz, 2H, CH2-Br), 1.28 (s, 9H, 3× CH3), 13C-NMR (101 MHz,
CDCl3) δ 174.7 (C=O), 137.9 (d, O-CH=), 109.6 (d, =CH-CH2), 39.2 (C(CH3)3), 38.9 (C(CH3)3); 27.1 (q, 3×
C(CH3)3), 23.6 (t, CH2-Br);
3-Bromoprop-1-en-1-yl naphthoate (3e). A solution of PPh3 (3.05 g, 11.6 mmol, 1.00 equiv) in dry DCM
(6 mL) was cooled to 0 °C via an ice-bath before Br2 (0.60 mL, 11.6 mmol, 1.00 equiv) was added
dropwise as a solution in dry DCM (6 mL), keeping the temperature at 0 °C. PPh3Br2 started to
precipitate. After complete addition of Br2, naphthoic acid was added to the reaction mixture. The
previously formed precipitate got dissolved and stirring was continued at rt for 1 hour. The solvent
was evaporated and the residue was treated with Et2O/hexane (1:1, 25 ml), which led to the formation
of a lot of precipitate, which was filtered and the solvent was evaporated. The product was obtained
by distillation under reduced pressure as a colorless liquid (2.00 g, 73%): bp 175 °C, 1.7 mbar (lit.35
129-132 °C, 2 Torr) and used without further purification. Acrolein (95%, 0.57 mL, 8.51 mmol, 1.00
equiv) was dissolved in dry DCM (20 mL) and cooled to -20 °C, using an acetone cooling bath. Then,
naphthoic acid bromide (2.00 g, 8.51 mmol, 0.95 equiv) was added and subsequently anhydrous ZnCl2
(12 mg, 0.09 mmol, 0.01 equiv). The reaction mixture was stirred and allowed to warm to rt and kept
stirring at this temperature for 30 min. The whole mixture was poured onto H2O/ice and the product
was extracted with Et2O (200 mL). Phases were separated and the organic layer washed with sat.aq.
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NaHCO3 and brine. Then it was dried over MgSO4 and the solvent evaporated, leaving a brown liquid,
which solidified upon storage at -18 °C (2.05 g, 81%). The product 3e was used without further
purification. ratio of E/Z = 1:1.3; 1H-NMR (400 MHz, CDCl3) δ 8.97 (t, J = 7.8 Hz, 1.1H, Ar), 8.33 (dd, J =
16.9, 7.3 Hz, 1H, Ar), 8.09 (t, J = 8.0 Hz, 1.2H, Ar), 7.91 (dd, J = 8.1, 4.9 Hz, 1.3H, Ar), 7.78 (d, J = 12.4
Hz, 0.4H, =CH-O (E)), 7.72 – 7.61 (m, 1.2H, Ar, =CH-O (Z)), 7.62 – 7.48 (m, 3H, Ar), 5.93 (dt, J = 12.4, 8.4
Hz, 0.4H, =CH- (E)), 5.43 (td, J = 8.4, 6.3 Hz, 0.6H, =CH- (Z)), 4.23 (d, J = 8.4 Hz, 1.2H, CH2-Br (Z)), 4.11
(d, J = 8.4 Hz, 0.9H, CH2-Br (E)); 13C-NMR (101 MHz, CDCl3) δ 163.7 (C=O(E)), 163.3 (C=O(Z)), 139.6 (=CH-
O (E)), 137.8 (=CH-O (Z)), 134.84 (ArCH), 134.78 (ArCH), 134.0 (ArC), 133.98 (ArC), 131.74 (ArC), 131.72
(ArC), 131.42 (ArCH), 131.40 (ArCH), 128.88 ( ArCH), 128.86 ( ArCH), 128.5 (ArCH), 128.4 (ArCH), 126.7
(ArCH), 126.6 (ArCH), 125.72 (ArCH), 125.67 (ArCH), 125.0 (ArC), 124.8 (ArC), 124.65 (ArCH), 124.56
(ArCH), 111.9 (=CH- (E)), 110.1 (=CH- (Z)), 28.9 (CH2-Br (E)), 24.0 (CH2-Br (Z)).
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L-Erythrose (6). Acetonide 520 (2.70 g, 16.9mmol, 1.00 equiv) was taken up in H2O (27 mL) and Dowex-
H+ (freshly washed with H2O) was added and the mixture was heated to 80 °C for around 30 min. The
mixture was allowed to cool to rt, was filtered over Celite, washed with fresh water (3×) and was
lyophilized to give pure L-erythrose (2.02 g, quant.) according to NMR, observed in a mixture of two
furanose forms and around ~10% of the open chain form as hydrate which is consistent with the
literature.36
4-O-Formyl-2,3-O-isopropylidene-L-erythrose (7). Solid SiO2-NaIO4 (14 % (w/w), 52 g, 2.00 equiv) was
added to a solution of 3,4-O-isopropylidine L-arabinose20 2 (3.20 g, 16.8 mmol, 1.00 equiv) in DCM
(65 mL) in one portion at rt. The flask was closed and shaken vigorously. After stirring 30 min at room
temperature, reaction monitoring via TLC (LP/EtOAc 1:1) showed complete conversion of starting
material to a more apolar and smearing spot (staining differently). The reaction mixture was filtered
and the silica gel was washed with fresh DCM (250 mL). After evaporation of the solvent, the product
7 was obtained as a colorless oil in good purity and used without further purification (2.50 g, 79%): Rf
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0.58 (LP/EtOAc 1:1); H-NMR (400 MHz, CDCl3) δ 9.69 (d, J = 2.2 Hz, 1H, H1), 8.01 (q, J = 0.8 Hz, 1H,
OCHO), 4.58 (ddd, J = 8.3, 5.1, 3.5 Hz, 1H, H3), 4.44 (m, 2H, H4a & H2), 4.07 (ddd, J = 12.2, 5.1, 0.8 Hz,
1H, H4b), 1.55 (s, 3H, CH3), 1.38 (s, 3H, CH3); 13C-NMR (101 MHz, CDCl3) δ 201.2 (C1), 160.1 (OCHO),
111.4 (C(CH3)2), 80.4 (C2), 75.9 (C3), 60.9 (C4), 26.9 (CH3), 24.9 (CH3).
1,2-Dideoxy-L-manno-hept-1-enitol (8a). The L-manno enitol peracetate 9a (540 mg, 1.39 mmol, 1.00
equiv) was dissolved in dry MeOH (20 mL) and NaOMe (8 mg, 0.14 mmol, 0.10 equiv) was added. After
1 hour TLC analysis (LP/EtOAc 3:1, DCM/MeOH 4:1) indicated all material was converted to the target
compound. The reaction mixture was neutralized by addition of freshly washed ion exchange resin,
filtered, washed with fresh MeOH and evaporated to leave pure target compound (195 mg, 79%) as a
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colorless oil, which solidified during storage. m.p.: 97.9-99.0 °C (Et2O); Rf 0.31 (DCM/MeOH 4:1); [D

-22 (c 1.0, MeOH); 1H NMR (400 MHz, MeOD) δ 6.05 (ddd, J = 17.2, 10.6, 5.7 Hz, 1H, H2), 5.34 (dt, J =
17.3, 1.7 Hz, 1H, H1a), 5.19 (dt, J = 10.6, 1.6 Hz, 1H, H1b), 4.21 – 4.13 (m, 1H, H3), 3.84 – 3.76 (m, 2H,
H5, H7a), 3.73 – 3.58 (m, 3H, H4, H6, H7b); 13C NMR (101 MHz, MeOD) δ 140.4 (C2), 115.9 (C1), 74.3
(C3), 73.4 (C4), 73.0 (C6), 71.5 (C5), 65.1 (C7); HRMS (+ESI-TOF) m/z [M + H] calcd for C7H15O5 179.0914,
found 179.0921; HRMS (-ESI-TOF) m/z [M - H]- calcd for C7H13O5 177.0768, found 177.0772;
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1,2-Dideoxy-L-gluco-hept-1-enitol (8b). The L-gluco peracetate 9b (50 mg, 0.129 mmol, 1.00 equiv) was
dissolved in dry MeOH (2 mL) and NaOMe (1 mg, 0.013 mmol, 0.1 equiv) was added, pH was checked
to be basic. After 1 h TLC analysis (LP/EtOAc 3:1 and DCM/MeOH 4:1) indicated all material was
converted to the target compound. The reaction mixture was neutralized by addition of freshly
washed (MeOH) acidic ion exchange resin, filtered washed with fresh MeOH and evaporated to leave
pure target compound (21 mg, 92%) which solidified on drying from Et2O solution. m.p.: 104.0-105.9
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°C (Et2O); Rf 0.31 (DCM/MeOH 4:1); [D  +9.4 (c 1.0, MeOH); 1H NMR (400 MHz, MeOD) δ 5.91 (ddd,
J = 17.3, 10.5, 6.8 Hz, 1H, H2), 5.35 (ddd, J = 17.3, 1.8, 1.3 Hz, 1H, H1a), 5.19 (ddd, J = 10.5, 1.9, 1.0 Hz,
1H, H1b), 4.19 (t, J = 6.9 Hz, 1H, H3), 3.78 (dd, J = 11.1, 3.4 Hz, 1H, H7a), 3.75 – 3.65 (m, 2H, H4, H6),
3.60 (dd, J = 11.1, 5.8 Hz, 1H, H7b), 3.56 (dd, J = 8.3, 1.3 Hz, 2H, H5); 13C NMR (101 MHz, MeOD) δ
139.2 (C2), 117.2 (C1), 75.8 (C3), 73.9, 72.9 (C4, C6), 72.6 (C5), 64.9 (C7). found 179.0921; HRMS (-ESI-
TOF) m/z [M - H]- calcd for C7H13O5 177.0768, found 177.0770;
1,2-Dideoxy-L-allo-hept-1-enitol (8c) and 1,2-Dideoxy-L-altro-hept-1-enitol (8d). Dry EtOH (150 mL)
was heated to 45 °C in a round bottom flask. In immediate succession, first indium (3.05 g, 26.6 mmol,
2.00 equiv), freshly distilled bromopropenyl acetate 3a (8.49 g, 39.9 mmol, 3.00 equiv) and
subsequently aldehyde 7 (2.50 g, 13.3 mmol, 1.00 equiv) was added as a solution in little EtOH in one
portion. Heating was removed and the temperature rose to approximately 60 °C. After the
temperature began to decrease again, the reaction mixture was stirred at 45 °C for 30 min, when TLC
(LP/EtOAc 1:1) showed complete conversion to a more apolar product (staining blue; starting material
yellow). The reaction mixture was filtered and the filtrate was evaporated, leaving a white residue,
which was taken up in pyridine (80 mL) and treated with acetic anhydride (60 mL) forming a solution.
Next, DMAP (20 mg, 0.16 mmol) was added and stirring was continued at rt overnight, when TLC
analysis (LP/EtOAc 1:1) indicated complete conversion. The reaction mixture was immersed into an
ice-bath and MeOH (80 mL) was added and the reaction mixture was stirred for ten min, before it was
diluted with EtOAc and transferred to a separatory funnel. The organic layer was washed with ice-cold
1N HCl, water, sat. aq. NaHCO3, brine and dried over MgSO4. The solvent was evaporated leaving a
slightly yellow, highly viscous liquid (5.70 g). This residue was taken up in dry MeOH and treated with
NaOMe until a pH of 9-10 was reached. The mixture was stirred at rt for 1 hour, when reaction
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monitoring via TLC (LP/EtOAc 1:1 & 1:3) showed complete conversion to very polar spot. The reaction
mixture was neutralized by addition of Dowex-H+ resin and filtered. Fresh (MeOH washed) Dowex-H+
(9.4 g) was added and the reaction mixture was stirred at rt for 24 h, when reaction monitoring via
TLC (DCM/MeOH 9:1) showed complete conversion to unprotected enitoles. Dowex-H+ was filtered,
the solvent was evaporated and the residue was taken up in H2O, washed with DCM, EtOAc and Et2O
and the aqueous layer was evaporated (2.50 g). The crude material was purified vacuum column
chromatography on silica gel (40 g, DCM/MeOH 9:1  4:1) to give targeted enitoles 8c and 8d in
inseparable mixture as a highly viscous, colorless oil (1.75 g, 73%, allo:altro ~9:1):
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Analytical data for 8c (L-allo): Rf: 0.71 (CHCl3/MeOH/H2O 14:6:1); H-NMR (400 MHz, MeOD) δ 6.01
(ddd, J = 17.2, 10.5, 6.6 Hz, 1H, H2), 5.32 (ddd, J = 17.3, 2.1, 1.3 Hz, 1H, H1a), 5.21 (ddd, J = 10.5, 2.0,
1.1 Hz, 1H, H1b), 4.28 (ddt, J = 6.1, 4.7, 1.2 Hz, 1H, H3), 3.91 – 3.73 (m, 2H, H5 & H7a), 3.73 – 3.57 (m,
3H, H4, H6 & H7b); 13C-NMR (101 MHz, MeOD) δ 138.5 (C2), 117.0 (C1), 76.5 (C6), 75.1 (C3), 74.3 (C5),
74.0 (C4), 64.1 (C7); HRMS (+ESI-TOF) m/z [M + Na]+ calcd for C7H14NaO5 201.0733, found 201.0752.
1,2-Dideoxy-L-manno-hept-1-enitol hexaacetate (9a) and 1,2-Dideoxy-L-gluco-hept-1-enitol
hexaacetate (9b). Freshly prepared L-erythrose 6 (0.371 g, 3.09 mmol, 1.00 equiv) was dissolved in dry
EtOH (30 mL) and was heated to 40 °C. First, indium (0.709 g, 6.18 mmol, 2.00 equiv) and then
bromopropenyl acetate 3a (1.69 g, 9.26 mmol, 3.00 equiv) was added and the mixture was stirred for
10 min. According to TLC (DCM/MeOH 4:1) all starting material was converted to a less polar material
(staining blue). The reaction mixture was filtered, evaporated, taken up in pyridine (5 mL) and Ac2O
(3.7 mL, 37.0 mmol, 12.0 equiv) was added under ice-bath cooling. After 15 min, a small amount of
DMAP was added and stirring was continued at rt overnight. Upon complete conversion (TLC,
DCM/MeOH 4:1, LP/EtOAc 1:1), the excess of Ac2O was quenched by addition of MeOH (10 mL) at 0
°C and stirring at rt for 30 min. The reaction mixture was diluted with EtOAc (150 mL) and was washed
with ice-cold 1N HCl, sat. aq. NaHCO3 and brine, dried over Na2SO4 and evaporated. The crude material
was subjected to column chromatography (90 g SiO2, 50 mL/min flow rate, 50 mL fractions, gradient
of LP/EtOAc 15% to 33%) to give the main manno-isomer 9a as first eluting compound in pure form
(646 mg, 54%). As second isomer the allo-isomer 9c was eluted close to the last eluting gluco-isomer
9b which could be isolated in a yield of 330 mg (28%, ~5% 9c) which could be purified to homogeneity
by trituration in MeOH (70 mg). The overall yield of isolated manno/gluco/allo heptenitol
peracetates (9a-c) is 841 mg (70%).
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Analytical data for 9a (manno): Rf 0.59 (LP/EtOAc 1:1); [α]D -23 (c 1.0, CHCl3); H NMR (400 MHz,
CDCl3) δ 5.68 (ddd, J = 17.2, 10.3, 7.7 Hz, 1H, H2), 5.46 (dd, J = 9.1, 2.4 Hz, 1H, H5), 5.37 – 5.32 (m, 1H,
H1a), 5.30 (dd, J = 8.3, 2.4 Hz, 1H, H4), 5.26 (dt, J = 10.3, 0.9 Hz, 1H, H1b), 5.19 (app. t, J = 8.0 Hz, 1H,
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H3), 5.09 (ddd, J = 9.1, 5.2, 2.7 Hz, 1H, H6), 4.19 (dd, J = 12.5, 2.7 Hz, 1H, H7a), 4.06 (dd, J = 12.5, 5.3
Hz, 1H, H7b), 2.07 (s, 3H, CH3C=O), 2.04 (s, 3H, CH3C=O), 2.03 (s, 3H, CH3C=O), 2.02 (s, 6H, 2×CH3C=O);
13C NMR (101 MHz, CDCl3) δ 170.7, 169.96, 169.95, 169.7, 169.6 (5×CH3C=O), 132.4 (C2), 121.1 (C1),
71.8 (C3), 69.8 (C4), 68.1 (C6), 67.5 (C5), 62.1 (C7), 21.1, 20.9, 20.82, 20.78, 20.7 (5×CH3C=O); HRMS
(+ESI-TOF) m/z [M + H]+ calcd for C17H25O10 389.1442, found 389.1445.
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Analytical data for 9b (gluco): m.p.: 113.2-114.7°C (MeOH); Rf 0.55 (LP/EtOAc 1:1); [D -28 (c 1.0,

CHCl3); 1H NMR (400 MHz, CDCl3) δ 5.77 (ddd, J = 17.1, 10.4, 6.3 Hz, 1H, H2), 5.45 – 5.23 (m, 5H, H1a/b,
H3, H4, H5), 5.07 (ddd, J = 7.7, 5.6, 3.2 Hz, 1H, H6), 4.24 (dd, J = 12.4, 3.2 Hz, 1H, H7a), 4.07 (dd, J =
12.4, 5.6 Hz, 1H, H7b), 2.12 (s, 3H, CH3C=O), 2.071 (s, 3H, CH3C=O), 2.066 (s, 3H, CH3C=O), 2.053 (s, 3H,
CH3C=O), 2.051 (s, 3H, CH3C=O); 13C NMR (101 MHz, CDCl3) δ 170.6, 170.0, 169.9, 169.8, 169.6
(5×CH3C=O), 131.3 (C2), 120.6 (C1), 72.8, 70.7, 68.76 (C3, C4, C5), 68.64 (C6), 61.8 (C7), 21.0, 20.93,
20.86, 20.83, 20.7 (5×CH3C=O); HRMS (+ESI-TOF) m/z [M + H]+ calcd for C17H25O10 389.1442, found
389.1447.
L-mannose (10) L-manno heptenitol 8a (170 mg, 0.96 mmol) was subjected to ozonolysis (general
method 1) to give L-mannose 10 as colorless oil (167 mg, 97%): Rf 0.21 (CHCl3/MeOH/H2O 14:6:1);
[α]D25 = -6.7 (c 1.0, H2O, 18h), lit.6 -13.5 (c 1.0, H2O); Spectral data in accordance with a commercially
sample of D-mannose (see supporting information).
L-glucose (11). L-gluco heptenitol 8b (18 mg, 0.101 mmol) was subjected to ozonolysis (general method
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1) to give L-glucose 11 as colorless oil (16 mg, 88%): Rf 0.12 (CHCl3/MeOH/H2O 14:6:1); [α]D -33 (c
1.0, H2O, 18h), lit.37(D-glucose) +50.1 (c 0.7, H2O); Spectral data in accordance with a commercially
sample of D-glucose (see supporting information).
L-Allose (12). L-Allo heptenitol (containing ~10% L-altro isomer) 8c (1.45 g, 8.15 mmol, 1.00 equiv) was
subjected to ozonolysis (general method 1) to give L-allose (containing ~10% L-altrose) (1.49 g, quant.).
A part of the crude material (1.00 g) was recrystallized from EtOH (2 mL) to give 0.63 g (70% recovery)
of pure L-allose as white needles. 12 (L-allose): mp 132 – 135 °C (lit.38 131 °C); Rf 0.39
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(CHCl3/MeOH/H2O 14:6:1); [α]D -13.7 (c 1.4 H2O, 24 h) [lit.6 [α]D -10.8° (c 1.4, H2O); H-NMR (400
MHz, D2O) δ 4.86 (d, J = 8.2 Hz, 1H, H1), 4.14 (t, J = 3.1 Hz, 1H, H3), 3.85 (dd, J = 12.2, 2.1 Hz, 1H, H6a),
3.76 (ddd, J = 10.0, 5.9, 2.3 Hz, 1H, H5), 3.71 – 3.56 (m, 3H, H6b & H4), 3.38 (dd, J = 8.2, 3.0 Hz, 1H,
H2); 13C-NMR (101 MHz, D2O) δ 94.0 (C1), 74.2 (C5), 71.9 (C3*), 71.8 (C2*), 67.4 (C4), 61.8 (C6); HRMS
(+ESI-TOF) m/z [M + Na]+ calcd for C6H12NaO6 203.0526, found 203.0527; Signals marked with an
asterisk could not be assigned undoubtedly. Spectral data in accordance with a commercially sample
of D-allose (see supporting information). L-altrose was identified in the mother liquid by comparison
with a commercially sample of D-altrose (see supporting information).
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4O-Acetyl-2,3O-isopropylidene-D-threose (15)24. 4-O-Acetyl-2,3O-isopropylidene-D-threitol24 (1.00 g,
4.90 mmol, 1.00 equiv) was dissolved in EtOAc (20 mL) in a microwave vial, IBX (4.12 g, 14.7 mmol,
3.00 equiv) was added and atmosphere was changed to argon. The vial was heated in the microwave
oven (Biotage Initiator) at 120 °C for 15 min. After filtration, the filtrate was concentrated (~5 mL) and
the obtained solution had to be used without further purification in the acyloxyallylation experiments
(16c). 1H-NMR (400 MHz, CD2Cl2) δ 9.76 (d, J = 1.7 Hz, 1H, H1), 4.33 (dd, J = 11.3, 4.0 Hz, 1H, H4a), 4.30
– 4.26 (m, 1H, H3), 4.19 (dd, J = 7.0, 1.6 Hz, 1H, H2), 4.13 (dd, J = 11.4, 5.0 Hz, 1H, H4b), 2.07 (s, 3H,
CH3C=O), 1.48 – 1.46 (m, 3H, C(CH3)2), 1.42 – 1.40 (m, 3H, C(CH3)2); 13C-NMR (101 MHz, CD2Cl2) δ 200.6
(C1), 170.4 (CH3C=O), 111.9 (C(CH3)2), 81.8 (C2), 74.8 (C3), 63.6 (C4), 26.5 (C(CH3)2), 26.0 (C(CH3)2), 20.5
(CH3C=O). Spectral data in accordance with literature.
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1,2-Dideoxy-D-gulo-hept-1-enitol (16a). D-Gulo pentaacetate 17a (0.71 g, 1.82 mmol, 1.00 equiv,
containing ~10% D-talo isomer 17c was dissolved in dry MeOH (20 mL) and NaOMe (10 mg, 0.18 mmol,
0.10 equiv) was added. The reaction mixture was stirred at rt for 20 min, when TLC analysis showed
complete conversion to a more polar spot (LP/EtOAc 3:1; DCM/MeOH 4:1). The reaction mixture was
neutralized with Dowex-H+ resin and filtered. Evaporation of the solvent gave the target compound
16a as a colorless oil (330 mg, quant., containing ~10% D-talo isomer 16c): Rf 0.26 (CHCl3/MeOH/H2O
14:6:1); 1H-NMR (400 MHz, MeOD) δ 6.03 (ddd, J = 17.3, 10.6, 5.9 Hz, 1H, H2), 5.33 (dt, J = 17.3, 1.7
Hz, 1H, H1a), 5.19 (ddd, J = 10.5, 2.0, 1.3 Hz, 1H, H1b), 4.17 (ddt, J = 7.2, 5.9, 1.4 Hz, 1H, H3), 3.85 (dd,
J = 4.4, 2.6 Hz, 1H, H5), 3.75 (dt, J = 6.1, 4.6 Hz, 1H, H6), 3.67 (dd, J = 11.2, 4.8 Hz, 1H, H7a), 3.59 (dd, J
= 11.2, 6.1 Hz, 1H, H7b), 3.53 (dd, J = 7.0, 2.6 Hz, 1H, H4); 13C-NMR (101 MHz, MeOD) δ 139.9 (C2),
116.3 (C1), 75.6 (C4), 74.8 (C6), 74.1 (C3), 71.0 (C5), 64.2 (C7); HRMS (-ESI-TOF) m/z [M - H]- calcd for
C7H13O5 177.0768, found 177.0782;
1,2-Dideoxy-D-ido-hept-1-enitol (16b). D-Ido pentaacetate 17b (0.16 g, 0.41 mmol, 1.00 equiv) was
dissolved in dry MeOH (10 mL) and NaOMe (2 mg, 0.04 mmol, 0.10 equiv) was added. The reaction
mixture was stirred at rt for 20 min, when reaction monitoring showed complete conversion to a very
polar spot (LP/EtOAc 3:1; DCM/MeOH 4:1). The reaction mixture was neutralized with Dowex-H+ resin
and then filtered. Evaporation of the solvent gave deacetylated enitole species 16b as a colorless oil
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(72 mg, quant.): Rf 0.30 (CHCl3/MeOH/H2O 14:6:1); [α]D +9.4 (c 0.7, MeOH); H-NMR (400 MHz,
MeOD) δ 5.95 (ddd, J = 17.0, 10.5, 6.4 Hz, 1H, H2), 5.35 (dt, J = 17.3, 1.7 Hz, 1H, H1a), 5.19 (ddd, J =
10.5, 1.8, 1.3 Hz, 1H, H1b), 4.24 (td, J = 6.3, 5.6, 1.3 Hz, 2H, H3), 3.78 (dt, J = 6.2, 4.5 Hz, 1H, H6), 3.69
(t, J = 3.8 Hz, 1H, H5), 3.66 (dd, J = 11.3, 4.8 Hz, 1H, H7a), 3.61 (dd, J = 11.3, 6.1 Hz, 1H, H7b), 3.57 (dd,
J = 5.5, 3.6 Hz, 1H, H4); 13C-NMR (101 MHz, MeOD) δ 139.2 (C2), 116.9 (C1), 75.8 (C4), 74.6 (C3), 74.0
(C6), 72.0 (C5), 64.2 (C7); HRMS (-ESI-TOF) m/z [M - H]- calcd for C7H13O5 177.0768, found 177.0777;
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1,2-Dideoxy-D-talo-hept-1-enitol (16c). Dry Ethanol (40 mL) was heated to 45 °C in a round bottom
flask. In immediate succession, indium (2.25 g, 19.6 mmol, 4.00 equiv), freshly distilled
bromopropenyl acetate 3a (5.26 g, 29.41 mmol, 6.00 equiv) and subsequently crude aldehyde 15 (<4.9
mmol, as a solution in little EtOAc) were added in one portion and the mixture was stirred at 45 °C.
TLC analysis (LP/EtOAc 1:1) showed complete conversion of starting material to a more apolar spot
(staining blue; starting material yellow) after 30 min. The reaction was filtered and the filtrate was
evaporated, leaving a white residue, which was taken up in pyridine (2.37 mL, 29.4 mmol, 6 equiv),
treated with Ac2O (1.50 mL, 14.7 mmol, 18 equiv) and stirred at rt. After 30 min, DMAP (6 mg,
0.05 mmol, 0.01 equiv) was added and it was stirred over night at rt, when TLC analysis (PE/EA 1:1)
indicated complete conversion. The reaction mixture was immersed into an ice-bath and MeOH (5 mL)
was added and the reaction mixture was stirred for ten min, before it was diluted with EtOAc (200 mL)
and washed with ice-cold 1N HCl, water, sat. aq. NaHCO3, brine and dried over NaSO4. Solvent was
evaporated and the residue was taken up in dry MeOH (40 mL) and NaOMe (2 mg, 0.04 mmol,
0.10 equiv) was added. The reaction mixture was stirred at rt for 20 min, when reaction monitoring
via TLC (LP/EtOAc 3:1; DCM/MeOH 4:1) showed complete conversion to a more polar spot. The
reaction mixture was neutralized with Dowex-H+ resin and filtered. After the solvent was evaporated,
the residue was taken up in dry MeOH (40 mL), fresh Dowex-H+ (4 g) added and stirred at rt over night,
when TLC analysis (DCM/MeOH 5:1) showed complete conversion to unprotected enitoles. The resin
was filtered and solvent evaporated. A pure fraction of the main isomer, talo-enitole 16c was isolated
by recrystallization in dry EtOH (7 mL) as a white, highly crystalline solid (150 mg, 17%). The remaining
isomers were isolated as a mixture from the mother liquid via flash column chromatography (45 g
SiO2, DCM/MeOH 6:1  2:1) as white solid. Overall yield of isolated enitoles 16a-d: 550 mg, 63% (from
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4-O-acetyl-2,3O-isopropylidene-D-threitol). Ratio of isomers (via H-NMR): talo (16c) 394 mg (72%),
galacto (16d) 116 mg (21%), gulo (16a) 29 mg (5%), ido (16b) 10 mg (2%); Analytical data for 16c (talo):
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m.p.: 146-147 °C (EtOH); Rf 0.23 (DCM/MeOH 5:1); [α]D +0.8 (c 1.0, MeOH); H-NMR (400 MHz,
MeOD) δ 6.02 (ddd, J = 17.2, 10.5, 6.7 Hz, 1H, H2), 5.32 (ddd, J = 17.3, 2.1, 1.3 Hz, 1H, H1a), 5.21 (ddd,
J = 10.5, 2.1, 1.1 Hz, 1H, H1b), 4.29 (ddt, J = 7.0, 4.7, 1.3 Hz, 1H, H3), 3.91 (td, J = 6.3, 1.7 Hz, 1H, H6),
3.70 (dd, J = 8.4, 4.7 Hz, 1H, H4), 3.62 (d, J = 6.3 Hz, 2H, H7a/b), 3.53 (dd, J = 8.4, 1.6 Hz, 1H, H5); 13C-
NMR (101 MHz, MeOD) δ 138.3 (C2), 117.2 (C1), 75.4 (C3), 74.8 (C4), 72.7 (C5), 71.9 (C6), 64.7 (C7).
HRMS (-ESI-TOF) m/z [M - H]- calcd for C7H13O5 177.0768, found 177.0779.
1,2-Dideoxy-D-gulo-hept-1-enitol hexaacetate (17a) and 1,2-Dideoxy-D-ido-hept-1-enitol hexaacetate
(17b). Dry Ethanol (30 mL) was heated to 45 °C in a round bottom flask. In immediate succession,
indium (0.75 g, 6.50 mmol, 2.00 equiv), freshly distilled bromopropenyl acetate 3a (1.75 g, 9.75 mmol,
3.00 equiv) and subsequently D-threose 14 (390 mg, 3.25 mmol, 1.00 equiv) was added as a solution
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in a little EtOH and the mixture was stirred vigorously at 45 °C. TLC analysis (DCM/MeOH 4:1) showed
complete conversion of starting material to a more apolar spot after 30 min. The reaction was filtered
and the solvent evaporated, leaving a white residue, which was taken up in pyridine (5 mL, 61.9 mmol,
19 equiv.), treated with Ac2O (3.6 mL, 39.0 mmol, 12 equiv.) and DMAP (4 mg, 0.03 mmol, 0.01 equiv)
over night, when TLC analysis (LP/EA 1:1) showed full conversion to more apolar spots. Excessive Ac2O
was quenched by addition of MeOH (5 mL) under ice-bath cooling before the reaction mixture was
diluted with EtOAc (200 mL) and washed with ice-cold 1N HCl, water, sat. aq. NaHCO3, brine and dried
over NaSO4. The solvent was evaporated and the crude material was subjected to column
chromatography on silica gel (90 g, LP/EtOAc 3:1  1:1) to give the main gulo-isomer 17a as first
eluting compound with little talo-isomer 17c (806 mg, 64%, containing 10% D-talo isomer). As third
eluting compound, the ido-isomer 17b was isolated as a pure (195 mg, 15%).
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Analytical data for 17a (gulo): Rf 0.68 (LP/EtOAc 2:1); 1H-NMR (400 MHz, CDCl3) δ 5.78 (ddd, J = 17.2,
10.3, 8.0 Hz, 1H, H2), 5.48 – 5.29 (m, 4H, H1a/b, H4 & H5), 5.29 – 5.18 (m, 2H, H3, H6), 4.35 (dd, J =
12.1, 4.0 Hz, 1H, H7a), 3.96 (dd, J = 12.1, 6.2 Hz, 1H, H7b), 2.10 (s, 3H, CH3C=O), 2.09 (s, 3H, CH3C=O),
2.07 (s, 3H, CH3C=O), 2.05 (s, 3H, CH3C=O), 2.03 (s, 3H, CH3C=O); 13C-NMR (101 MHz, CDCl3) δ 170.5,
170.3, 170.1, 169.9, 169.7 (5×CH3C=O), 131.6 (C2), 121.7 (C1), 72.4 (C3), 70.7 (C4), 69.4 (C6), 68.7 (C5),
62.0 (C7), 21.05, 20.95, 20.9, 20.8, 20.7 (5×CH3C=O); HRMS (+ESI-TOF) m/z [M + Na]+ : calcd for
C17H24NaO10 411.1262 found 411.1277.
17b (Ido) (195 mg, 15%): Rf 0.55 (LP/EtOAc 2:1); [α]D20 +14 (c 1.0, CHCl3); 1H-NMR (400 MHz, CDCl3) δ
5.75 (ddd, J = 16.8, 10.6, 6.1 Hz, 1H, H2), 5.43 (tt, J = 6.0, 1.2 Hz, 1H, H3), 5.40 – 5.28 (m, 3H, H1a/b &
H5), 5.28 – 5.19 (m, 2H, H4* & H6*), 4.31 (dd, J = 12.1, 4.1 Hz, 1H, H7a), 4.03 (dd, J = 12.1, 5.8 Hz, 1H,
H7b), 2.098 (s, 3H, CH3C=O), 2.095 (s, 3H, CH3C=O), 2.09 (s, 3H, CH3C=O), 2.08 (s, 3H, CH3C=O), 2.05 (s,
3H, CH3C=O); 13C-NMR (101 MHz, CDCl3) δ 170.5, 170.05, 169.95, 169.68, 169.65 (5×CH3C=O), 131.4
(C2), 120.1 (C1), 72.5 (C3), 71.0 (C4*), 69.6 (C6*), 68.8 (C5), 62.0 (C7), 21.0, 20.9, 20.80, 20.78, 20.75
(5×CH3C=O); HRMS (+ESI-TOF) m/z [M + Na]+: calcd for C17H24NaO10 411.1262, found 411.1268. Signals
marked with an asterisk could not be assigned undoubtedly.
D-Gulose (18). D-Gulo heptenitol 16a (300 mg, 1.69 mmol, containing ~10% D-talo isomer 16c) was
subjected to ozonolysis (general method 1) to give D-gulose 18 (300 mg, quant containing ~10% D-
talose 20): Rf 0.28 (CHCl3/MeOH/H2O 14:6:1); 1H-NMR (400 MHz, D2O) δ 4.89 (d, J = 8.4 Hz, 1H, H1),
4.07 (t, J = 3.5 Hz, 1H, H3), 4.00 (ddd, J = 6.8, 5.4, 1.4 Hz, 1H, H5), 3.83 – 3.80 (m, 1H, H4), 3.75 (dd, J =
6.2, 2.0 Hz, 2H, H6a/b), 3.63 (ddd, J = 8.4, 3.4, 0.5 Hz, 1H, H2); 13C-NMR (101 MHz, D2O) δ 94.4 (C1),
74.4 (C5), 71.8 (C3), 70.0 (C4), 69.7 (C2), 61.6 (C6). HRMS (-ESI-TOF) m/z [M + COOH]- calcd for C7H13O8
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225.0616, found 225.0632; Spectral data is in accordance with commercially available D-gulose (see
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D-Idose (19). D-Ido heptenitol 16b (70 mg, 0.39 mmol) was subjected to ozonolysis (general method 1)
to give D-idose 19 (70 mg, quant.): Rf 0.30 (CHCl3/MeOH/H2O 14:6:1); [α]D20 = +11 (c 0.7, H2O, 24 h),
commercial sample: +11 (c 1.0, H2O); Spectral data is in accordance with commercially available D-
idose (see supporting information).
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D-Talose (20). D-Talo heptenitol 16c (100 mg, 0.56 mmol) was subjected to ozonolysis (general method
1) to give D-talose 20 (100 mg, quant.): Rf 0.22 (CHCl3/MeOH/H2O 14:6:1); [α]D20 +19 (c 1.0, H2O, 18 h),
lit.39 +25 (c 1.0, H2O); Spectral data in accordance with a commercially sample of D-talose (see
supporting information).
D-Talose (20) and D-Galactose (21).A mixture of D-talo heptenitol 16c and D-galacto heptenitol 16d
(8:2, 60 mg, 0.34 mmol) was subjected to ozonolysis (general method 1) to give a mixture of
D-talose 20 and D-galactose 21 (61 mg, quant.): Rf 0.22 (CHCl3/MeOH/H2O 14:6:1); Spectral data in
accordance with a commercially sample of D-talose and D-galactose (see supporting information).
3-Iodoprop-1-en-1-yl pivalate (22). Chloropropenyl pivalate32 (2.00 g, 11.3 mmol, 1.00 equiv) was
dissolved in acetone (4 mL) and added to a stirred solution of NaI (3.40g, 22.6 mmol, 2.00 equiv) in
acetone (20 mL). A strong exotherm was observed at the beginning of the addition and the solution
turned yellow. Further addition was done under water bath cooling. The reaction mixture was stirred
at rt and under exclusion of light. After 1 h a small sample was diluted with Et2O, washed with water
1
and brine, dried over Na2SO4 and was evaporated to and analyzed by H-NMR to confirm full
conversion to the target compound. The reaction mixture was poured into Et2O (~100 mL) and the
white precipitate was filtered and washed with fresh Et2O. The filtrate was washed with water, brine,
dried over Na2SO4 and evaporated to leave a crude material (2.7 g, 89%) as a red liquid, according to
1H and 13C-NMR the target compound 22 in sufficient purity to be subjected to the acyloxyallylation
experiments without prolonged storage. ratio of E/Z 1:2; (E)-isomer 1H-NMR (400 MHz, CDCl3) δ 7.41
(dt, J = 12.3, 1.0 Hz, 1H, =CH-O), 5.76 (dt, J = 12.3, 8.7 Hz, 1H, =CH-CH2), 3.90 (dd, J = 8.7, 1.0 Hz, 2H,
CH2-I), 1.23 (s, 9H, C(CH3)3); 13C-NMR (101 MHz, CDCl3) δ 175.2 (C=O), 138.4 (=CH-O) 112.9 (=CH-CH2),
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38.9 (C(CH3)3), 27.0 (C(CH3)3), 0.7 (CH2-I). (Z)-isomer: H-NMR (400 MHz, CDCl3) δ 7.11 (d, J = 6.1 Hz,
1H, =CH-O), 5.29 (td, J = 8.7, 6.1 Hz, 1H, =CH-CH2), 3.96 (dd, J = 8.7, 0.7 Hz, 2H, CH2-I), 1.30 (s, 9H,
C(CH3)3); 13C-NMR (101 MHz, CDCl3) δ 174.7 (C=O), 137.3 (=CH-O), 111.1 (=CH-CH2), 39.3 (C(CH3)3), 27.2
(C(CH3)3), -4.7 (CH2-I).
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Acknowledgements
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We thank the Austrian Science Fund FWF for financial support (Grants: J 3449-N28).
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Associated Content
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Supporting Information
Details of the quantification of the enitol ratios 16a-d and 17a-d via 1H-NMR
Detailed investigation of the fate of reagents 3a and 23 under the reaction conditions
1H-NMR and 13C-NMR spectra for known and new compounds (3c-d, 6-7, 8a-c, 9a-b, 15, 16a-d, 17a-b
and 22) and comparisons of 1H-NMR and 13C-NMR spectra of synthesized and commercial samples of
compounds 10-13 and 18-21.
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(22) Schiaffo, C. E.; Dussault, P. H. J. Org. Chem. 2008, 73, 4688.
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(25) Noteworthy, for the conversion of the corresponding primary alcohol to
aldehyde 15, from a variety of oxidation conditions only a protocol with IBX in EtOAc was
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prevented the decomposition of very labile aldehyde 15 otherwise observed.
(26) Due to insufficient resolution at GC-level, quantification was based on the 1H-
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experiments processed by a passage over a short bed of SiO2 and collection of all entitol
fractions.
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surface and tentatively the initial rate of organyl formation - to achieve clean and full
conversion which was reported to be unsuccessful with standard indium quality at prolonged
reaction times.
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(33) Acyloxyallylations performed with zinc were acetylated using Ac2O in pyridine,
followed by acidic aqueous workup to remove all inorganics.
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