13C NMR Spectra of 1-Stearoyl-2-Linoleyl-sn-Glycero-3-Phosphorylcholine and 1-Stearoyl-2-Arachidonoyl-sn-Glycero-3-Phosphorylcholine in CDCl3 Solution and in Sonicated Dispersions in 2H2O

Article abstract of DOI:10.1002/mrc.1270180211

Two mixed-acid lecithins: 1-stearoyl-2-linoleyl-sn-glycero-3-phosphorylcholine (SLL) and 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (SAL) have been synthesized by phospholipase A₂ digestion of 1,2-distearoyl-sn-glycero-3-phosphorylcholine (DSL), followed by reacylation of the lysolecithin with the desired fatty acid anhydride. ¹³C (25.2 MHz) NMR spectra of SLL and SAL in CDCl3 solution and in sonicated dispersions in ²H2O have been obtained.Complete spectral assignments are reported for the two molecules in both systems. ¹³C nuclear spin-lattice relaxation times (T₁) af SLL and SAL in sonicated aqueous dispersions have also been measured.Relaxation rate profiles as a function of the chain segment position are in general agreement with those recently obtained from ²H NMR for similar systems.