MICROBIAL DEGRADATION OF DEOXYCHOLIC ACID BY PSEUDOMONAS sp. NCIB 10590. CHARACTERISATION OF PRODUCTS AND A POSTULATED PATHWAY

Article abstract of DOI:10.1016/S0040-4020(01)88910-9

Minor products in the microbial degradation of deoxychlolic acid are characterised and a metabolic pathway proposed.In a previous publocation we reported the transformation of deoxycholic acid 1 by Pseudomonas sp.NCIB 10590, in a mineral salts medium, to a mixture of neutral and acidic steroids.The major neutral product, 12β-hydroxyandrosta-1,4-dien-3,17-dione 2 and the major acidic product 12α-hydroxypregna-1,4-dien-3-one-20-carboxylic acid 9 were identified.We now report the structures of the minor products and propose a metabolic pathway.