Discovery of bicycloalkyl urea melanin concentrating hormone receptor antagonists: Orally efficacious antiobesity therapeutics

Article abstract of DOI:10.1021/jm049035q

Melanin concentrating hormone (MCH) is involved in regulation of food intake and energy homeostasis. Antagonists of the MCH receptor are expected to affect food intake and weight gain, making MCH-R1 an attractive target for obesity treatment. Herein, we r

Full text of DOI:10.1021/jm049035q

Supporting Information
Discovery of Bicycloalkyl Urea Melanin Concentrating Hormone (MCH) Receptor Antagonists as
Orally Efficacious Antiobesity Therapeutics.
Mark D. McBriar,* Henry Guzik, Ruo Xu, J aroslava Paruchova, Shengjian Li,† Anandan Palani,
J ohn W. Clader, William J . Greenlee, Brian E. Hawes, Timothy J . Kowalski, Kim O’Neill, Brian
Spar, and Blair Weig
Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, New J ersey 07033-0539
†Current address: J ohnson and J ohnson Pharmaceutical Research Institute, Raritan, New J ersey.
Synthetic procedures and characterization data for compounds 16, 17, 23, 24, 26, 28, 29-31, and 38-40.
Representative experimental:
Compounds 16 and 17:
Step 1
O
O
OH
CN
10
3-Bromobenzonitrile (20 g, 0.11 mole) was dissolved in THF (500 mL) and the solution was
cooled to –100 oC. A solution of n-butyllithium (1.6 M in hexane, 68 mL, 0.11 mole) was added via an
additional funnel over one hour. During this time, the temperature inside the reaction flask was kept below
–95 oC. After addition was complete, the reaction was stirred at –95 oC for 10 minutes. A solution of 1, 4-
dioxaspiro[4, 5]decan-8-one (17.1g, 0.11 mole) in THF (100 mL) was added over one hour. During this
time the reaction temperature was kept below –75 oC. The reaction was then stirred for 30 minutes and the
temperature slowly rose to –25 oC. The reaction was then quenched with H2O and diluted with EtOAc.
The organic layer was washed with water (3x), dried over Na2SO4, filtered and concentrated in vacuo. The
residue was recrystallized from EtOAc/Hex to give rise to 15.5 g pure product as a white powder. The
crude product from mother liquor was purified by flash chromatography (30% EtOAc/Hex) to provide an
additional 8.0g (Total yield of 10: 23.5 g, 82%). 1H NMR (300 MHz, CDCl3) δ 7.85 (s, 1 H), 7.76 (d, J =
7.7 Hz, 1 H), 7.54 (d, J = 7.7 Hz, 1 H), 7.45 (t, J = 7.7 Hz, 1 H), 3.99(t, J = 2.8 Hz, 4H), 2.0-2.2 (m, 4 H),
1.6-1.8 (m, 4 H).
Step 2
1

O
O
CN
CN
11
12
Alcohol 10 (10 g, 39 mmol) was dissolved in TFA (80 mL). The mixture was heated to 50 oC for
4 h. Trifluoroacetic acid was removed under vacuum and the residue was partitioned between EtOAc and
saturated aqueous NaHCO3. The organic layer was washed with water (2x) and dried over Na2SO4, filtered
and concentrated in vacuo. Flash chromatography (15% EtOAc/Hex) provided 4-(3-cyanophenyl)-3-
cyclohexene-1-one (11, 3.1 g, 41%) 1H NMR (300 MHz, CDCl3) δ 7.4-7.7 (m, 4H), 6.17 (t, J = 3.9 Hz,
1H). 3.11(s, 2H), 2.90 (t, J = 6.6 Hz, 2H), 2.67 (t, J = 6.6 Hz, 2 H) and 4-(3-cyanophenyl)-2-cyclohexene-1-
one (12, 4.0 g, 53%), 1H NMR (300 MHz, CDCl3) δ 7.47-7.63 (m, 4 H), 6.92 (d, J = 9.9 Hz, 1H), 6.92 (dd,
J = 10.4, 2.7 Hz, 1H), 3.80 (m, 1H), 2.3-2.6 (m, 3H), 2.25 (m, 1H).
Step 3
O
CN
13
The mixture of 11 and 12 (1.8 g, 9.1 mmol) was dissolved in EtOAc (50 mL). The catalyst, 10 %
Pt/C (0.5 g, 0.9 mmol) was then added and mixture was shaken at 45 psi hydrogen overnight. The catalyst
was filtered and solvent was removed under vacuum. The residue was dissolved in CH2Cl2 (100 mL) and
Dess-Martin periodinane (4.2 g, 10 mmol) was added. After 5 h, the solution was washed with water (3x),
dried over Na2SO4, filtered and concentrated in vacuo. Flash chromatography (15% EtOAc/Hex) provided
1
13 (1.1g, 63%) as a clear oil. H NMR (300 MHz, CDCl3) δ 7.4-7.6 (m, 4 H), 3.10 (t, J = 12.1, 3.3 Hz,
1H), 2.53 (m, 4H), 2.3 (m, 2H), 1.94 (m, 2H).
Step 4
2

CF3
CF3
F
F
NH
NH
OH
N
OH
N
O
N
O
N
17
16
CN
CN
A solution of 13 (0.7 g, 3.5 mmol), N-(2-aminoethyl)-3-(S)-hydroxy-pyrrolidine18 (1.36 g, 10.5
mmol) and sodium triacetoxyborohydride (2.2 g, 10.5 mmol) were refluxed in CH2Cl2 (50 mL). After 24 h,
the organic layer was washed with water (3x), dried over sodium sulfate and concentrated in vacuo. The
crude reaction mixture (60mg) and 3-chloro-4-(trifluoromethyl)phenyl isocyanate (50 mg, 0.29 mmol) were
stirred in CH2Cl2 (5 mL). After 24 h, the reaction solution was loaded directly onto a preparative TLC
plate and purified (15% EtOAc/Hex) to provide 16 (35.9 mg, 36%). 1H NMR (300 MHz, CDCl3) δ 10.72
(s, 1H), 7.57-7.71 (m, 4H), 7.42-7.53 (m, 2H), 7.03 (t, J = 9.8 Hz, 1H), 4.48 (m, 1H), 4.29 (t, J = 12.0, 4.0
Hz, 1H), 3.00-3.17 (m, 4H), 2.66-2.85 (m, 4H), 1.76-2.52 (m, 8H), 1.60-1.70 (m, 2H), 1.30-1.45 (m, 2H).
LCMS: 519.1, rt. = 5.46 min (M+H+), 98% purity; HRMS (FAB) m/z 519.2392 [(M+H)+; calcd for
1
C27H30F4N4O2: 519.2305], and 17 (38.6 mg, 39%). H NMR (300 MHz, CDCl3) δ10.73 (s, 1H), 7.67-7.73
(m, 2H), 7.36-7.51 (m, 4H), 7.03 (t, J = 9.8 Hz, 1H), 4.51 (m, 1H), 4.24 (t, J = 12.0, 4.0 Hz, 1H), 3.35 (t, J
= 4.0 Hz, 2H), 3.10 (m, 1H), 2.75-2.90 (m, 4H), 2.52 (m, 2H), 2.22 (m, 1H), 1.80-2.00 (m, 6H), 1.45-1.70
(m, 4H). LCMS: 519.1, rt. = 5.46 min (M+H+), 98% purity; HRMS (FAB) m/z 519.2387 [(M+H)+; calcd
for C27H30F4N4O2: 519.2305].
Compounds 23 and 24:
Step 1
O
CO2Et
CN
19
A solution of 3-acetylbenzonitrile (10.2 g, 70 mmol) in t-butanol (100 mL) was treated with potassium t-
butoxide (11.8 g, 105 mmol) potionwise and the reaction was stirred for 0.5 h. Ethyl chloroacetate (9.4 g,
77 mmol) was added dropwise and the reaction was stirred at room temperature overnight. The reaction
mixture was diluted with EtOAc, washed with brine, dried over Na2SO4, filtered and concentrated in vacuo.
1
Flash chromatography (30% EtOAc/Hex) provided 19 (4.9 g, 30%) as a clear oil. H NMR (300 MHz,
CDCl3) δ 7.40-7.80 (m, 4H), 4.20-4.40 (m, 1H), 3.86-4.00 (m, 1H), 3.70 (s, 0.5H), 3.41 (s, 0.5H), 1.78 (s,
1.5H), 1.76 (s, 1.5H), 1.34 (t, J = 7.14 Hz, 1.5H), 0.96 (t, J = 7.14 Hz, 1.5H).
Step 2
3

CHO
CN
20
Sodium hydroxide (0.73 g, 32 mmol) was dissolved in 20 mL EtOH and the reaction was cooled
to 0 oC. A solution of 19 (4.9 g, 21.2 mmol) in 20 mL EtOH was added and the reaction was stirred for 0.5
h. Water (2 mL) was added and the reaction was stirred at room temperature overnight. The reaction
mixture was concentrated to remove ethanol, diluted with water (45 mL) and 12 N HCl (8.4 mL) and
heated to 100 oC for 1 h. The reaction was cooled to room temperature and extracted with CH2Cl2. The
organic layer was washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. Flash
1
chromatography (30% EtOAc/Hex) gave 20 (0.46 g, 14%) as a yellow oil. H NMR (300 MHz, CDCl3)
δ 9.69 (s, 1H), 7.45-7.65 (m, 4H), 3.70 (q, J = 7.14 Hz, 1H), 1.49 (d, J = 7.14 Hz, 3H).
Step 3
O
CN
21
A solution of 20 (0.46 g, 2.9 mmol) 1:1 Et2O/EtOH (40 mL) was cooled to 0oC. Potassium
hydroxide (5 mg, 0.09 mmol) and methyl vinyl ketone (0.3 g, 4.35 mmol) were added. After 18 h, the
reaction mixture was concentrated in vacuo and the residue was dissolved in CH2Cl2. The organic layer was
washed with brine, dried over Na2SO4, filtered and concentrated in vacuo to provide crude 21 (0.91 g). 1H
NMR (300 MHz, CDCl3) δ7.40-7.70 (m, 4H), 6.88 (d, J = 9.9 Hz, 1H), 6.17 (d, J = 9.9 Hz, 1H), 2.15-2.50
(m, 4H), 1.58 (s, 3H).
Step 4
O
CN
22
A solution of crude 21 in EtOAc (50 mL) was treated with platinum on carbon (5%, 0.5 g) and the
mixture was shaken at 45 psi hydrogen over the weekend. The reaction mixture was concentrated in vacuo
and dissolved in CH2Cl2 (50 mL). Dess-Martin periodinane (1.7 g, 4.0 mmol) was added. After 12 h, the
reaction mixture was washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. Preparative
thin layer chromatography (30% EtOAc/Hex) afforded 22 (0.36 g, 77% for three steps) 1H NMR (300
MHz, CDCl3) δ 7.46-7.76 (m, 4H), 2.36-2.50 (m, 4H), 2.18-2.32 (m, 2H), 1.92-2.08 (m, 2H), 1.34 (s, 3H).
4

Following procedures similar to those described for compounds 16 and 17, the following compounds were
prepared:
Compound 23: 1H NMR (300 MHz, CDCl3) δ 10.7 (s, 1H), 7.40-7.80 (m, 6H), 7.06 (t, J = 9.9 Hz, 1H),
4.55 (m, 1H), 4.14 (m, 1H), 1.16-3.12 (m, 18H), 1.32 (s, 3H). LCMS: 533.3, rt. = 3.64 min (M+H+), 99%
purity; HRMS (FAB) m/z 533.2541 [(M+H)+; calcd for C28H33F4N4O2: 533.2540].
Compound 24: 1H NMR (300 MHz, CDCl3) δ 10.7 (s, 1H), 7.40-7.80 (m, 6H), 7.06 (t, J = 9.9 Hz, 1H),
4.49 (m, 1H), 4.30 (m, 1H), 1.16-3.12 (m, 18H), 1.14 (s, 3H). LCMS: 533.3, rt. = 3.61 min (M+H+), 99%
purity; HRMS (FAB) m/z 533.2552 (M+H)+; calcd for C28H33F4N4O2-533.2540.
Compounds 28 and 29:
Step 1
O
O
CN
25
A solution of alcohol 10 (20 g, 77 mmol) and triethylamine (15.6 g, 154 mmol) in CH2Cl2 (500
mL) was treated with a solution of methanesulfonyl chloride (9.7 g, 85 mmol) in CH2Cl2 (100 mL) over
one hour. After 5h, additional triethylamine and mesyl chloride (same amount as the first time) were added.
After 1 additional hour, the reaction was quenched with saturated aqueous NaHCO3, washed with water
(2x), dried over Na2SO4, filtered and concentrated in vacuo. The residue was recrystallized (EtOAc/Hex) to
give rise to 9.1 g pure 25. Flash chromatorgraphy of the filtrate (20% EtOAc/Hex) give rise to additional
7.8 g (Total yield:16.9 g, 91%). 1H NMR (300 MHz, CDCl3) δ 7.66 (s, 1H), 7.61 (d, J = 8.2 Hz, 1H), 7.50
(d, J = 7.7 Hz, 1H), 7.40 (t, J = 7.7 Hz, 1H), 6.06 (m, 1H), 4.03 (s, 4H), 2.64 (m, 2H), 2.44 (m, 2H), 1.93 (t,
J = 6.6 Hz, 2H).
Step 2
O
O
CN
27
A solution of diethylzinc (1 M in hexane, 19 ml, 19 mmol) in CH2Cl2 at 0 oC was treated with a
solution of trifluoroacetic acid (2.1 g, 19 mmol) in CH2Cl2 (20 mL). After 20 minutes, a solution of
diiodomethane (5 g, 19 mmol) in CH2Cl2 (10 mL) was then added, followed by a solution of 25 (1.5 g, 6.2
mmol) in CH2Cl2 (20 mL). After 18h, the reaction mixture was quenched with 1N HCl. The organic layer
was separated, washed with water (2x), dried over Na2SO4, filtered and concentrated in vacuo. Flash
5

chromatography (10% EtOAc/Hex) gave 27 (0.8 g, 50%) as a clear oil. 1H NMR (300 MHz, CDCl3) δ 7.60
(s, 1H), 7.53 (d, J = 7.7 Hz, 1H), 7.45 (d, J = 7.7 Hz, 1H), 7.36 (t, J = 7.7 Hz, 1H), 3.9 (m, 4H), 2.10-2.35
(m, 2H), 1.4-1.9 (m, 4H), 1.25 (s, 1H), 1.05 (m, 1H), 0.82 (t, J = 5.5 Hz, 1H).
Step 3
O
CN
41
A solution of 27 (0.8 g, 3.1 mmol) was stirred in 1:1 CH2Cl2/TFA (20 mL). After 30 min, the
solvent was removed and the residue was diluted with EtOAc and saturated aqueous Na2CO3. The organic
layer was separated, washed with water (2x), dried over Na2SO4, filtered and concentrated in vacuo. Flash
chromatography (10% EtOAc/Hex) gave 41 (0.56 g, 85%) as a white solid. 1H NMR (300 MHz, CDCl3)
δ 7.60 (s, 1H), 7.49-7.51 (m, 2H), 7.42 (t, J = 7.7 Hz, 1H), 2.62-2.92 (m, 2H), 2.39-2.52 (m, 2H), 2.18-2.32
(m, 2H), 1.48-1.57 (m, 1H), 1.10 (d, J = 7.7 Hz, 2H).
Starting from compounds 11 and 41, the following compounds were synthesized using the same method as
that of compounds 16 and 17:
Compound 26: 1H NMR (300 MHz, CDCl3) δ 11.05 (bs, 1H), 7.38-7.65 (m,5 H), 7.19 (m, 1H), 7.02 (t, J =
8.8 Hz, 1 H), 6.11 (s, 1H), 4.52 (m, 1H), 3.35 (m, 2H), 2.82-1.70 (m, 16 H). LCMS: 467.2, rt. = 5.26 min
(M+H+), 99% purity; HRMS (FAB) m/z 467.2021 [(M+H)+; calcd for C26H29ClFN4O: 467.2014].
Compound 28: 1H NMR (300 MHz, CDCl3) δ 10.75 (s, 1H), 7.67-7.73 (m, 2H), 7.33-7.53 (m, 4H), 7.05 (t,
J = 9.3 Hz, 1H), 4.53 (m, 1H), 4.15 (dd, J = 11.5, 3.8 Hz, 1H), 3.35 (m, 2H), 3.11 (m, 1H), 2.75-2.92 (m,
4H), 2.55 (m, 1H), 1.36-2.30 (m, 9H), 1.08 (dd, J = 9.3, 4.9 Hz, 1H), 0.84 (t, J = 5.5 Hz, 1H). LCMS:
497.2, rt. = 5.06 min (M+H+), 98% purity; HRMS (FAB) m/z 497.2126 [(M+H)+; calcd for C27H31ClFN4O2
497.2119].
Compound 29: 1H NMR (300 MHz, CDCl3) δ 10.71 (s, 1H), 7.67-7.78 (m, 2H), 7.34-7.53 (m, 4H), 7.05 (t,
J = 9.3 Hz, 1H), 4.53 (m, 1H), 4.21 (m, 1H), 3.27 (m, 2H), 3.10 (m, 1H), 2.72-2.92 (m, 4H), 2.55 (m, 1H),
1.36-2.30 (m, 9H), 0.99 (dd, J = 9.3, 4.9 Hz, 1H), 0.76 (t, J = 5.5 Hz, 1H). LCMS: 497.2, rt. = 5.11 min
(M+H+), 100% purity; HRMS (FAB) m/z 497.2125 [(M+H)+; calcd for C27H31ClFN4O2 497.2119].
Diastereomers of 29 were separated via preparative HPLC using a chiral AD column (20% i-PrOH/Hex,
isocratic), flow rate 40 mL/min to provide (+)-30 and (-)-31:
Compound (+)-30: [α]25D +41.5° (c 1.0, MeOH); rt. = 160 min, 100% purity; HRMS (FAB) m/z 497.2125
[(M+H)+; calcd for C27H31ClFN4O2 497.2119].
Compound (-)-31: [α]25D -43.8° (c 1.0, MeOH); rt. = 137 min, 100% purity; HRMS (FAB) m/z 497.2125
[(M+H)+; calcd for C27H31ClFN4O2 497.2119].
6

Compound 38:
Step 1
O
CN
34
A solution of 3-bromobenzonitrile (26.8 g, 147.1 mmol) in THF (1000 mL) at –78 °C was treated
with a solution of n-butyllithium (2.5 M in hexanes; 61.0 mL, 155 mmol) such that the reaction temperature
remained ≤ -78 °C. After 15 min, a solution of 3-methoxy-2-cyclopenten-1-one (15 g, 134 mmol) in THF
(80 mL) was added such that the reaction temperature remained ≤ -78 °C. The reaction mixture was
warmed to -20 °C over 1.5 h, quenched with a solution of 1N HCl and concentrated in vacuo to remove
THF. A solution of 1N HCl (100 mL) was added, the solution was stirred for 45 min. and extracted with
EtOAc (3x). The combined organic extracts were washed with saturated aqueous NaHCO3, brine, dried
over MgSO4, filtered and concentrated in vacuo. The residue was crystallized at 0 °C from a solution of 1N
HCl, filtered, rinsed with cold 1N HCl, H2O and ether to provide 34 (14.4 g, 59%) as a pale yellow solid:
1H NMR (300 MHz, CDCl3) δ 7.90 (d, J = 1.6 Hz, 1H), 7.90 (d, J = 7.7 Hz, 1H), 7.74 (d, J = 7.7 Hz, 1H),
7.59 (d, J = 7.7 Hz, 1H), 6.63 (d, J = 1.6 Hz, 1H), 3.07-3.03 (m, 2H), 2.65-2.62 (m, 2H).
Step 2
OH
CN
42
A solution of 34 (7.88 g, 43.0 mmol) in methanol (100 mL) at 0 °C was treated with CeCl3•7H2O
(20.5 g, 55.0 mmol) followed portionwise by NaBH4 (2.10 g, 55.0 mmol). The reaction was warmed to
ambient temperature over 12 h, quenched with saturated aqueous NH4Cl and concentrated to remove
MeOH. The concentrate was diluted with H2O and extracted with EtOAc (3x). The combined organic
extracts were washed with saturated aqueous NaHCO3, brine, dried and concentrated in vacuo. Trituration
(10% EtOAc/Hex) at 0 °C and filtration afforded the alcohol 42 (6.39 g, 80%) as a white powder: 1H NMR
(300 MHz, CDCl3) δ 7.71 (d, J = 1.6 Hz, 1H), 7.70 (d, J = 7.7 Hz, 1H), 7.55 (dd, J = 7.7, 1.6 Hz, 1H), 7.45
(t, J = 7.7 Hz, 1H), 6.30 (dd, J = 3.8, 1.6 Hz, 1H), 5.05 (m, 1H), 2.90 (m, 1H), 2.65 (m, 1H), 2.56 (m, 1H),
1.92 (m, 1H), 1.62 (br s, 1H).
7

Step 3
OH
CN
35
A solution of allylic alcohol 42 (0.50 g, 2.7 mmol) in CH2Cl2 (75 mL) was treated with Et2Zn (1.0
M in hexanes; 14 mL, 14 mmol). After 10 min, the reaction mixture was cooled to 0 °C, treated with a
solution of CH2I2 (1.13 mL, 14 mmol) in CH2Cl2 (10 mL) dropwise over 10 min and allowed to warm to
ambient temperature. After 48h, the reaction mixture was quenched slowly with saturated aqueous NH4Cl
and stirred 10 min. The reaction mixture was extracted with CH2Cl2 (2x), and the combined organic phases
were washed with saturated aqueous NaHCO3, dried and concentrated in vacuo. Flash chromatography
(40% EtOAc/Hex) gave 35 (500 mg, 93%) as a clear oil: 1H NMR (300 MHz, CDCl3) δ 7.46-7.32 (m, 4 H),
4.69 (ddd, J = 12.1, 7.7, 4.4 Hz, 1H), 2.20-1.85 (m, 4H), 1.80 (br s, 1H), 1.38-1.25 (m, 2H), 0.85 (dd, J =
8.2, 5.5 Hz, 1H).
Step 4
O
CN
36
A solution of alcohol 35 (0.50 g, 2.51 mmol) in CH2Cl2 (25 mL) at 0 °C was treated with pyridine (445 µL,
5.50 mmol) followed by Dess-Martin periodinane (2.12 g, 5.0 mmol) and warmed to ambient temperature.
After 2h, 3 drops of H2O were added. After 30 min further, the reaction was quenched with saturated
aqueous NaHCO3, saturated aqueous Na2SO3, and extracted with CH2Cl2 (3x). The combined organic
phases were dried and concentrated in vacuo. Flash chromatography (25% EtOAc/Hex) gave 36 (440 mg,
89%) as a yellow solid: 1H NMR (300 MHz, CDCl3) δ 7.55-7.52 (m, 2 H), 7.47-7.43 (m, 2H), 2.45 (m,
1H), 2.40-2.25 (m, 3H), 2.17 (dd, J = 9.3, 5.5 Hz, 1H), 1.61-1.52 (m, 2H).
Step 5
8

F
CF3
NH
O
N
N
OH
CN
38
A solution of ketone 36 (80 mg, 0.41 mmol) in CH2Cl2 (1 mL) was treated with amine (+)-14 (79
mg, 0.61 mmol) followed by titanium tetraisopropoxide (145 µL, 0.49 mmol). After 18 h, the reaction
mixture was diluted with MeOH (1 mL) and sodium borohydride (31 mg, 0.81 mmol) was added. After 2.5
h further, the reaction mixture was diluted with a solution of saturated aqueous sodium/potassium tartrate
and extracted with CH2Cl2 (4x). The combined organic phases were dried and concentrated in vacuo. The
crude product was dissolved in CH2Cl2 (4 mL) and treated with diisopropylethyl amine (78 µL, 0.45 mmol)
followed by 4-fluoro-(3-trifluoromethyl)phenyl isocyanate (61 µL, 0.43 mmol). After 18h, the reaction
mixture was diluted with saturated aqueous NaHCO3 and extracted with CH2Cl2 (3x). The combined
organic phases were dried and concentrated in vacuo. Preparative thin layer chromatography (5% MeOH/
CH2Cl2), followed by filtration, an EtOH rinse and concentration in vacuo furnished 38 (100 mg, 48% over
2 steps) as a clear oil: 1H NMR (300 MHz, CDCl3) δ : 1H NMR (300 MHz, CDCl3) δ 10.68 (d, J = 14.3 Hz,
1H), 7.71-7.68 (m, 2H), 7.46-7.36 (m, 4H), 7.04 (dd, J = 9.9, 9.9 Hz, 1H), 5.06 (ddd, J = 9.9, 7.1, 3.3 Hz,
1H), 4.50 (m, 1H), 3.50 (dd, J = 15.4, 7.1 Hz, 1H), 3.41(dd, J = 15.4, 7.1 Hz, 1H), 3.13 (ddd, J = 15.9,
15.4, 8.2 Hz, 1H), 2.98-2.71 (m, 4H), 2.55 (ddd, J = 15.9, 15.9, 8.8 Hz, 1H), 2.42 (br s, 1H), 2.28-1.80 (m,
5H), 1.72 (ddd, J = 7.7, 7.1, 3.3 Hz, 1H), 1.34-1.19 (m, 2H), 0.94 (dd, J = 7.1, 6.0 Hz, 1H); LCMS: 517.1,
rt. = 4.50 min (M+H+), 98.3% purity; HRMS (FAB) m/z 517.2250 [(M+H)+; calcd for C27H29N4O2F4:
517.2227].
Anal. calcd for C27H28N4O2F4·HCl·0.5H2O: C, 57.70; H, 5.38, N, 9.97. Found: C, 57.78; H, 5.19; N, 9.80.
Following procedures similar to those described for compound 38, the following compounds were
prepared:
Compound 39: 1H NMR (300 MHz, CDCl3) δ : 1H NMR (300 MHz, CDCl3) δ 11.24 (br s, 1H), 7.57-7.54
(m, 2H), 7.30-7.22 (m, 4H), 6.92 (s, 1H), 5.05 (s, 1H), 3.72-3.34 (m, 2H), 2.95-2.66 (m, 6H), 2.19-1.95 (m,
7H), 1.77 (ddd, J = 12.1, 8.2, 4.4 Hz, 1H), 1.35-1.19 (m, 2H), 0.99 (dd, J = 6.6, 6.0 Hz, 1H). LCMS: 483.1,
rt. = 5.27 min (M+H+), >99% purity; HRMS (FAB) m/z 483.1725 [(M+H)+; calcd for C26H29N4OCl2:
483.1718].
Compound 40: 1H NMR (300 MHz, CDCl3) δ : 1H NMR (300 MHz, CDCl3) δ 11.93 (br s, 1H), 8.80 (s,
1H), 7.99 (d, J = 4.4 Hz, 1H), 7.87 (dd, J = 8.2, 4.4 Hz, 1H), 7.71 (d, J = 8.2 Hz, 2H), 7.36 (d, J = 8.2 Hz,
2H), 4.85 (m, 1H), 3.78-3.17 (m, 8H), 2.81 (m, 4 H), 2.48 (dd, J = 2.2, 1.6 Hz, 1H), 2.15-1.98 (m, 6H), 1.82
(ddd, J = 13.2, 11.5, 7.7 Hz, 1H), 1.64 (dd, J = 4.9, 4.4 Hz, 1H), 1.43 (m, 1H), 1.08 (m, 1H). LCMS: 544.1,
rt. = 4.45 min (M+H+), >99% purity; HRMS (FAB) m/z 544.2699 [(M+H)+; calcd for C29H34N5OF4:
544.2699].
Anal. calcd for C29H33N5OF4·2HCl·H2O: C, 54.89; H, 5.88, N, 11.04. Found: C, 55.12; H, 5.85; N, 11.12.
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