Sequencing of peptoid peptidomimetics by Edman degradation

Article abstract of DOI:10.1016/S0040-4039(98)00556-5

The direct identification of resin-bound peptoid peptidomimetics by sequencing is described. The N-terminus of the peptoid was treated with phenyl isothiocyanate, after which the N-terminal peptoid residue was cleaved from the resin as its thiohydantoin derivative. For deduction of the peptoid sequence, the HPLC retention times of the obtained thiohydantoins were compared to those of independently prepared reference thiohydantoins.