PREPARATION OF 2'(3')-O-Acyl-pCpA DERIVATIVES AS SUBSTRATES FOR T4 RNA LIGASE-MEDIATED "CHEMICAL AMINOACILATION"

DOI: 10.1016/0040-4020(84)85106-6

Source and publish data:

Tetrahedron p. 87 - 94 (1984)

Update date:2022-08-02

Topics:

Authors:

Heckler, T.G.

Chang, L.H.

Zama, Y.

Naka, T.

Hecht, S.M.

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Article abstract of DOI:10.1016/0040-4020(84)85106-6

The synthesis of 2'(3')-O-(amino)acyl-pCpA derivatives is described and the products are characterized chemically and as substrates for T4 RNA ligase in the presence of E.coli tRNA^Phe-C_OH.The modified (N-acetylamino)acyl-tRNA's so derived are characterized by their chromatographic and biochemical properties, and by their ability to act as P-site t-RNA donors in the peptidyltransferase reaction.

Full text of DOI:10.1016/0040-4020(84)85106-6

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