Characterization of amino acids and steroids by fluorine-19 nuclear magnetic resonance spectrometry of p-fluorobenzoyl derivatives

Article abstract of DOI:10.1021/ac00279a023

The utility of p-fluorobenzoyl chloride as an analytical **1**9F NMR reagent to characterize sterols and amino acids is reported. The **1**9F chemical shift data for approximately 30 sterols and amino acids are presented. The interaction of the p-fluorobenzoyl group with the steroid ring system makes a significant contribution to the **1**9F chemical shift of these steroid derivatives. These results suggest a convenient technique for characterizing different steroids (e. g. , estrogens, pregnanes, androstanes, etc. ).