Inhibitory kinetics of novel 2,3-dihydro-1H-inden-1-one chalcone-like derivatives on mushroom tyrosinase

Article abstract of DOI:10.1016/j.bmcl.2015.10.071

In this study, novel 2,3-dihydro-1H-inden-1-one chalcone-like compounds and their hydroxyl derivatives were synthesized, and their inhibitory effects on mushroom tyrosinase activity were evaluated. Two of the compounds synthesized inhibited the diphenolas

Full text of DOI:10.1016/j.bmcl.2015.10.071

Bioorganic & Medicinal Chemistry Letters 25 (2015) 5495–5499
Contents lists available at ScienceDirect
Bioorganic & Medicinal Chemistry Letters
journal homepage: www.elsevier.com/locate/bmcl
Inhibitory kinetics of novel 2,3-dihydro-1H-inden-1-one
chalcone-like derivatives on mushroom tyrosinase
⇑
Sini Radhakrishnan , Ronald Shimmon, Costa Conn, Anthony Baker
School of Chemistry and Forensic Science, University of Technology Sydney, 15 Broadway, Ultimo, NSW 2007, Australia
a r t i c l e i n f o
a b s t r a c t
Article history:
In this study, novel 2,3-dihydro-1H-inden-1-one chalcone-like compounds and their hydroxyl derivatives
were synthesized, and their inhibitory effects on mushroom tyrosinase activity were evaluated. Two of
the compounds synthesized inhibited the diphenolase activity of tyrosinase in a dose dependent manner
Received 1 August 2015
Revised 20 October 2015
Accepted 23 October 2015
Available online 24 October 2015
and exhibited much higher tyrosinase inhibitory activities (IC50 values of 12.3
tively) than the positive control, kojic acid (IC50: 27.5 M). Kinetic analysis showed that their inhibition
was reversible. Both the novel compounds displayed competitive inhibition with their Ki values of
10.3 M and 8.7 M, respectively. This study suggests hydroxy substituted 2,3-dihydro-1H-inden-1-
lM and 8.2 lM, respec-
l
Keywords:
l
l
Tyrosinase
Competitive
Diphenolase
Reversible
one chalcone-like compounds to serve as promising candidates for use as depigmentation agents.
Ó 2015 Elsevier Ltd. All rights reserved.
Melanin, a polymeric dark pigment is produced by melanocytes
within specialized organelles called melanosomes. The melano-
somes contain several enzymes that mediate the production of
melanin.1,2 The conversion of tyrosine to melanin requires
tyrosinase, also known as polyphenol oxidase, a copper containing
protein. Tyrosinase [EC 1.14.18.1], is the key enzyme in the mela-
to 3,4-dihydroxy phenylalanine (L-DOPA) and oxidation of L-DOPA
to dopaquinone.3,4 However, an excessive accumulation of the
pigment could lead to serious aesthetic issues. Alterations in
tyrosinase function could culminate with serious dermatological
nogenic pathway responsible for the hydroxylation of
L-tyrosine
Table 1
Substitution pattern and tyrosinase inhibition effects of 1-indanone derivatives
O
O
R1
H
R1
a
+
O
O
R1
R4
Scheme 1. General method for synthesis of 2,3-dihydro-1H-inden-1-one chalcones.
Reagents and conditions: (a) MeOH, NaOH, 0 °C, 24 h.
R2
R3
Compound R1
R2
R3
R4
Yield
(%)
Inhibition rate (%) at
50
l
M
O
O
1a
1b
1c
1d
1e
1f
1g
2a
H
H
OMe
H
OMe
H
H
OH
H
NO2
H
H
NO2
H
OMe OMe
OMe
H
OH
OH
H
H
H
H
H
H
H
95.5
87.6
64.2
54.1
85.4
77.2
22.6 0.44
19.8 0.15
25.4 0.55
24.2 0.33
30.6 0.47
32.2 0.11
38.6 0.32
65.2 0.15
74.6 0.14
50.1 0.38
45.2 0.22
NO2
NO2
OMe
OMe
OH
b
OMe
Scheme 2. Dealkylation of methoxy chalcones. Reagents and conditions: (b) BBr3,
CH2Cl2.
H
OMe 70.3
H
H
OH
OH
OH
H
51.2
45.2
42.6
2b
2c
Kojic acid
⇑
Corresponding author. Tel.: +61 426687300.
H
E-mail
address:
Sini.KaranayilRadhakrishnan@student.uts.edu.au
(S. Radhakrishnan).
http://dx.doi.org/10.1016/j.bmcl.2015.10.071
0960-894X/Ó 2015 Elsevier Ltd. All rights reserved.

5496
S. Radhakrishnan et al. / Bioorg. Med. Chem. Lett. 25 (2015) 5495–5499
a structural perspective, tyrosinase has two copper ions in its
120
100
active site which play a vital role in its catalytic activity. At the
active site of tyrosinase, a dioxygen molecule binds in side-on
coordination between two copper ions. Each of the copper ions is
coordinated by three histidines in the protein matrix. The copper
atoms participate directly in hydroxylation of monophenols to
diphenols (cresolase activity) and in the oxidation of o-diphenols
to o-quinones (catechol oxidase activity) that enhance the produc-
tion of the brown color.16,17
Several natural and synthetic tyrosinase inhibitors have been
reported, including aromatic aldehydes and acids, tropolone,
arbutin, flavonoids, and kojic acid.18,19 However, most of these
compounds have been reported to be either toxic towards cells
or have low stability towards oxygen and water.20–22
Indanones are bioactive molecules that are frequently used as
precursors in the synthesis of pharmaceutical and natural product
substances. Previously, indanone based curcumin analogs have
been reported to have strong inhibitory effects on tyrosinase.23
Another indanone derivative, tetrahydrocurcumin, was recom-
mended to be used in cosmetics as a lighting agent.24
Although indole and its derivatives are popular medicinal
agents, the related heterocycles like indene and their derivatives
like substituted 1-indanones (2,3-dihydro-1H-inden-1-one) have
much less been exploited for their biological potential. Previously
we have studied the tyrosinase inhibition of azachalcone com-
pounds.25 Hence, we considered that it might be interesting to syn-
thesize a series of novel 2,3-dihydro-1H-inden-1-one chalcone-like
80
60
40
2a
2b
20
0
0
5
10
15
20
[I] (µM)
Figure 1. Inhibition effects of compounds 2a and 2b on the diphenolase activity of
mushroom tyrosinase. Data are presented as means (n = 3).
disorders including melasma, ephelides (freckles), solar lentigo
(age spots), and sites of actinic damage.5–7 Tyrosinase is responsi-
ble for melanization in animals, cuticle formation in insects,
wound healing and browning of fruits and vegetables.8–15 There-
fore, tyrosinase inhibitors have potential applications in the treat-
ment of dermatological disorders associated with melanin
hyperpigmentation, in agriculture as bio-insecticides and also in
cosmetics for whitening and depigmentation after sunburn. From
250
2a
200
150
100
50
0
1
2
3
4
0
0
2
4
6
8
10
12
[E] (µg/mL)
180
160
140
120
100
80
2b
0
1
2
3
4
60
40
20
0
0
2
4
6
8
10
12
[E] (µg/mL)
Figure 2. The inhibitory mechanism of compounds 2a and 2b on mushroom tyrosinase were reversible. The concentration of inhibitor used for curves 0–4 are 0, 0.25, 0.5, 1.0
and 2.0 M, respectively.
l

S. Radhakrishnan et al. / Bioorg. Med. Chem. Lett. 25 (2015) 5495–5499
5497
compounds and their hydroxyl derivatives for use as depigmenta-
tion agents and as anti-browning food additives.
was seen to be greater than the positive control kojic acid which
exhibited 45.2% inhibition at 50 M. The inhibitory activities of
l
In the first step, 2,3-dihydro-1H-inden-1-one chalcone-like
compounds were synthesized by the base catalyzed Claisen–
Schmidt condensation of 1-indanone and an appropriate ketone
in a polar solvent like methanol (Scheme 1). Some selected
methoxy chalcones were then successfully dealkylated to their
corresponding hydroxy compounds in the presence of boron
tribromide (BBR3) (Scheme 2).
these newly synthesized compounds were compared to that of
kojic acid as a reference standard and the results are shown in
Table 1.
Results indicated that both compounds 2a and 2b could inhibit
the diphenolase activity of tyrosinase in a dose-dependent manner.
With increasing concentrations of inhibitors, the remaining
enzyme activity decreased exponentially. The IC50 for compounds
In the present study, tyrosinase extracted from the edible
mushroom Agaricus bisporus is used due to its easy availability
and high homology with the mammalian enzyme that renders it
well suited as a model for studies on melanogenesis.26 We used
2a and 2b was estimated to be 12.3 lM and 8.2 lM, respectively
(Fig. 1).
The plots of the remaining enzyme activity versus the concen-
tration of enzyme at different inhibitor concentrations gave a
family of straight lines, which all passed through the origin. The
presence of inhibitor did not reduce the amount of enzyme, but
just resulted in the inhibition of enzyme activity. The results
showed that both the compounds 2a and 2b were reversible inhi-
L
-DOPA as substrate to study the effect of inhibitor compounds
on the oxidation of
L
-DOPA by tyrosinase (diphenolase activity).27
Kojic acid, a well-known tyrosinase inhibitor served as the positive
control. We have further investigated the kinetic parameters and
inhibition mechanisms of active tyrosinase inhibitor compounds.
Dose-dependent inhibition experiments were performed in tripli-
cate to determine the IC50 of the active inhibitors 2a and 2b.
In terms of the structure–activity relationships, compound 2b
((2Z)-2-(3,4-dihydroxybenzylidene)-2,3-dihydro-1H-inden-1-one)
exhibited the most potent tyrosinase inhibitory activity with
inhibition of 74.6%. This could be accounted to the presence of a
3,4-dihydroxy group in the B-ring that shows structural
bitors of mushroom tyrosinase for oxidation of L-DOPA (Fig. 2).
Compounds 1c, 2b, 2c and 2d exhibited moderate tyrosinase
inhibition while compounds 1a, 1b and 1d–1f showed negligible
tyrosinase inhibition in comparison with kojic acid. Previous stud-
ies have recognized potent inhibitory effect of chalcones with
hydroxyl groups on tyrosinase.28–34 The hydroxyl groups in com-
pounds carry out the nucleophilic attack on the coppers of tyrosi-
nase active site and directly involved in transferring protons during
catalysis, which resulted in inactivation of tyrosinase.35,36 Further-
more, results indicated that electron-donating groups contributed
more to the inhibitory activity of the compounds on mushroom
resemblance with the substrate,
L-DOPA. Compound 2a with an
ortho–para dihydroxy substitution on ring B showed an inhibition
of 65.2% at 50
lM. The inhibition of both compounds 2a and 2b
400
2a
300
200
100
0
-4
-2
0
2
4
6
-100
-200
1/S (mM/L)
400
300
200
100
0
2b
-4
-2
0
2
4
6
-100
-200
1/S (mM/L)
Figure 3. Lineweaver Burk plot for inhibition of compounds 2a and 2b on mushroom tyrosinase. Data were obtained as mean values of 1/V, the inverse of the absorbance
increase at a wavelength of 492 nm per min of three independent tests with different concentrations of -DOPA as a substrate. The concentration of compounds 2a and 2b
from top to bottom is 20 M, 5 M, 1.25 M and 0 M, respectively.
L
l
l
l
l

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S. Radhakrishnan et al. / Bioorg. Med. Chem. Lett. 25 (2015) 5495–5499
50
40
30
20
10
0
2b
-40
-30
-20
-10
0
10
20
30
-10
-20
-30
-40
[inhibitor] µM
40
30
20
10
2a
0
0
-40
-30
-20
-10
10
20
30
-10
-20
-30
[inhibitor] µM
Figure 4. Dixon plot for the inhibitory effect of compounds 2a and 2b on
L
-DOPA oxidation catalyzed by mushroom tyrosinase. The inhibitor concentrations were 0, 10
M.
lM
and 20 M, respectively. The -DOPA concentrations were 200, 400 and 600 l
l
L
tyrosinase than the electron-withdrawing groups. Electron donat-
ing groups (e.g., –OMe, –OH) on the atoms adjacent to the system
Table 2
Effect on mushroom polyphenol oxidase activity and kinetic analysis of compounds
p
activate the aromatic ring by increasing the electron density on the
ring through a resonance donating effect.
In this study, we investigated in depth the tyrosinase inhibitory
activities of compounds 2b and 2a. We measured the reaction rates
in the presence of active inhibitors with various concentrations of
Compound
Type of inhibition
IC50
(
l
M)
RA*
Ki#
(lM)
2a
2b
Kojic acid
Competitive
Competitive
Competitive
12.3 0.22
8.2 0.44
27.5 0.56
2.23F
3.35F
10.3
8.7
12.2
#
Values were measured at 5
constant).
RA is the relative inhibitory activity compared to the standard kojic acid where
1.0F means one time activity of kojic acid.
lM of active compounds and Ki is the (inhibitor
L-DOPA as a substrate. As the concentrations of active inhibitors 2a
*
and 2b increased, Km values gradually increased, but Vmax values
did not change, thereby indicating the inhibitors act as competitive
inhibitors of mushroom tyrosinase (Fig. 3).
The inhibition kinetics were illustrated by Dixon plots, which
were obtained by plotting 1/V versus [I] with varying concentra-
tions of substrate. Dixon plots gave a family of straight lines pass-
ing through the same point at the second quadrant, giving the
inhibition constant (Ki) (Fig. 4).
12.3 lM and 8.2 lM, respectively. Both the compounds exhibited
reversible competitive inhibition. Compound 2b with a 3,4-dihy-
droxy group in the B-ring had structural resemblance to the
substrate
L-DOPA. This leads to the competitive displacement of
The Ki value for the compounds 2a and 2b estimated from this
L-DOPA from the active site of the enzyme tyrosinase in a lock-
Dixon plot was 10.3
l
M and 8.7
l
M, respectively. A comparison of
and key model. In addition, the presence of the hydroxy group at
the 4-position (ring B) is indispensable for significant tyrosinase
inhibition in this class of compounds. These preliminary findings
justify pursuing further work on hydroxy substituted 1-indanone
chalcone-like compounds to serve as a potential scaffold for the
future development of active tyrosinase inhibitors.
Notes: Chemical reagents and instruments: Melting points (mp)
were determined with WRS-1B melting point apparatus and the
thermometer was uncorrected. NMR spectra were recorded on
Agilent 500 spectrometer at 25 °C in CDCl3 or DMSO-d6. All
the Ki values of the compounds with that of kojic acid revealed that
they possess much higher affinity to tyrosinase than kojic acid
(Table 2).
To summarize, in this study, we synthesized novel 2,3-dihydro-
1H-inden-1-one (1-indanone) chalcone-like compounds and
their hydroxy derivatives, and studied their inhibition on the
diphenolase activity of mushroom tyrosinase. Hydroxy substituted
1-indanone chalcone-like compounds 2a and 2b were found to be
significantly more potent than kojic acid with their IC50 values of

S. Radhakrishnan et al. / Bioorg. Med. Chem. Lett. 25 (2015) 5495–5499
5499
chemical shifts (o) are quoted in parts per million downfield from
TMS and coupling constants (J) are given in Hz. Abbreviations used
in the splitting pattern were as follows: s = singlet, d = doublet,
t = triplet, m = multiplet. HRMS spectra were recorded using the
Agilent Technologies 6520 LC/MS- QTOF. All reactions were
monitored by TLC (Merck Kieselgel 60 F254) and the spots were
visualized under UV light. Infrared (IR) spectra were recorded on
Thermo Scientific NICOLET 6700 FT-IR spectrometer. Tyrosinase,
velocity (1/V) versus inhibitor concentration (I) with varying con-
centrations of the substrate.
Supplementary data
Supplementary data associated with this article can be found, in
the online version, at http://dx.doi.org/10.1016/j.bmcl.2015.10.
071.
L-3,4-dihydroxyphenylalanine (L-DOPA) and kojic acid were
purchased from Sigma–Aldrich Chemical Co.
References and notes
General method for the synthesis of 2,3-dihydro-1H-inden-1-one
(1-indanone) chalcone-like compounds (1a–1g): To a stirred solution
of 1-indanone (2.0 mmol) and the corresponding aldehyde
(2.0 mmol) in methanol (6 mL) at room temperature was added
freshly pulverized sodium hydroxide (160 mg, 4.0 mmol). The
solution was stirred overnight (monitored by TLC) and then poured
onto crushed ice. Once all the ice had melted, pH was adjusted to 5
using hydrochloric acid (5 M). The resulting solution was then
extracted with ethyl acetate, washed with brine and filtered after
drying with anhydrous sodium sulfate. The solution was then
concentrated with a rotary evaporator under vacuum to give the
respective compound.
Method for the synthesis of hydroxy indanone compounds (2a–2c):
A solution of BBr3 (2.5 mL for each methoxy group) was added to a
cooled solution of the corresponding methoxy chalcone (1 mmol)
in CH2Cl2 under argon. The cooling bath was removed and the dark
solution was warmed to rt and stirred for 1–5 h. The dark solution
was then poured into ice water and filtered. The aqueous layer was
extracted with chloroform twice. The organic extract was washed
with water followed by brine and anhydrous sodium sulfate. This
was then rotary evaporated to give the respective product.
Enzyme activity assay: The mushroom tyrosinase inhibition
activity of 1-indanone chalcone-like compounds was measured
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medium containing 0.5 mM
L-DOPA in 50 mM Na2HPO4–NaH2PO4
buffer (pH 6.8). The final concentrations of tyrosinase was
3.33 mg/ml. The substrate reaction progress curve was analyzed
to obtain the reaction rate constants. The reaction was carried
out at 30 °C and pH 6.8.
Determination of the inhibition type of compounds 2a and 2b on
mushroom tyrosinase: Inhibitors were first dissolved in DMSO and
used for the test after a 30-fold dilution. The final concentration
of DMSO in the test solution was 3.33%. Mushroom tyrosinase
(50
trations of enzyme substrate and 50
then 50 L of different concentrations of tested samples were
l
L; 0.2 mg mLÀ1) was incubated with 50
lL of various concen-
l
L of phosphate buffer, and
l
simultaneously added to the reaction mixtures. The measurement
was performed in triplicate for each concentration and averaged
before further calculation. The absorbance variations from these
studies were used to generate Lineweaver–Burk plots to determine
the inhibition type. The kinetic parameter (Km) of the tyrosinase
activity was calculated by linear regression from Lineweaver–Burk
plots. For the type of enzyme inhibition and the inhibition constant
(Ki) for an enzyme-inhibitor complex, the mechanisms were ana-
lyzed by Dixon plot, which is a graphical method plot of 1/enzyme
36. Espin, J. C.; Varon, R.; Fenoll, L. G.; Gilabert, M. A.; Garcia-Ruiz, P. A.; Tudela, J.;
Garcia-Canovas, F. Eur. J. Biochem. 2000, 267, 1270.