β-CYANOETHYL N,N-DIALKYLAMINO/N-MORPHOLINOMONOCHLORO PHOSPHOAMIDITES, NEW PHOSPHITYLATING AGENTS FACILITATING EASE OF DEPROTECTION AND WORK-UP OF SYNTHESIZED OLIGONUCLEOTIDES

Article abstract of DOI:10.1016/S0040-4039(00)94216-3

β-Cyanoethyl monochlorophosphoamidites of the secondary amines N,N-dimethylamine, N,N-diisopropylamine and morpholine have been prepared which showed quantitative phosphitylation of the protected deoxynucleosides.The phosphitylated deoxynucleosides have successfully been used in the formation of internucleotidic bonds. β-Cyanoethyl nucleoside-3'-N,N-diisopropylphosphoamidites have saticfactorily been used for the synthesis of the oligodeoxynucleotide d(CGGTACCG).