The Cu2+-promoted cleavage of mRNA 5'-cap analogs: A kinetic study with p1(7-methylguanosin-5'-yl) p3-(nucleosid-5'-yl) triphospates and p1- (7-methylguanosin-5'-yl) p4-(guanosin-5'-yl) tetraphosphate

Article abstract of DOI:10.1080/07328319908045590

A kinetic study on the cleavage of a number of mRNA 5'-cap analogs, m⁷GpppN and m⁷GppppG, with Cu²⁺ aquo ion has been performed. Time- dependent product distributions at various pH and metal ion concentrations have been determined by capillary zone electrophoresis, and these data have been used to calculate the rate constants for various parallel reactions of the breakdown of the cap analogs.

Full text of DOI:10.1080/07328319908045590

This article was downloaded by: [Uppsala universitetsbibliotek]
On: 04 October 2014, At: 02:55
Publisher: Taylor & Francis
Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered
office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK
Nucleosides and Nucleotides
Publication details, including instructions for authors and
subscription information:
http://www.tandfonline.com/loi/lncn19
The Cu2+-Promoted Cleavage of
mRNA 5′-cap Analogs: A Kinetic Study
with P1-(7-Methylguanosin-5′-yl) P3-
(Nucleosid-5′-yl) Triphospates and P1-(7-
Methylguanosin-5′-yl) P4-(Guanosin-5′-
yl) Tetraphosphate
Zbigniew Wieczorek a , Edward Darzynkiewicz b , Satu Kuusela c &
Harri Lönnberg c
a Department of Physics and Biophysics , University of Agriculture
and Technology , 10-957, Olsztyn, Poland
b Department of Biophysics , Institute of Experimental Physics,
University of Warsaw , 93 Zwirki i Wigury, 02-089, Warsaw, Poland
c Department of Chemistry , University of Turku , FIN-20014, Turku,
Finland
Published online: 04 Oct 2006.
To cite this article: Zbigniew Wieczorek , Edward Darzynkiewicz , Satu Kuusela & Harri
Lönnberg (1999) The Cu2+-Promoted Cleavage of mRNA 5′-cap Analogs: A Kinetic Study with
P1-(7-Methylguanosin-5′-yl) P3-(Nucleosid-5′-yl) Triphospates and P1-(7-Methylguanosin-5′-
yl) P4-(Guanosin-5′-yl) Tetraphosphate, Nucleosides and Nucleotides, 18:1, 11-21, DOI:
10.1080/07328319908045590
To link to this article: http://dx.doi.org/10.1080/07328319908045590
PLEASE SCROLL DOWN FOR ARTICLE
Taylor & Francis makes every effort to ensure the accuracy of all the information (the
“Content”) contained in the publications on our platform. However, Taylor & Francis,
our agents, and our licensors make no representations or warranties whatsoever as to
the accuracy, completeness, or suitability for any purpose of the Content. Any opinions
and views expressed in this publication are the opinions and views of the authors,
and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content
should not be relied upon and should be independently verified with primary sources
of information. Taylor and Francis shall not be liable for any losses, actions, claims,

proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or
howsoever caused arising directly or indirectly in connection with, in relation to or arising
out of the use of the Content.
This article may be used for research, teaching, and private study purposes. Any
substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing,
systematic supply, or distribution in any form to anyone is expressly forbidden. Terms &
Conditions of access and use can be found at http://www.tandfonline.com/page/terms-
and-conditions