PERMEABLE MEMBRANE - MASS SPECTROMETRIC MEASUREMENT OF REACTION KINETICS.

Article abstract of DOI:10.1021/ac00230a013

The compound to be analyzed is introduced into the source of a mass spectrometer after selective permeation through a thin membrane which permits passage of apolar molecules. The in situ measurement of ethyl 2-furoate produced in an alpha -chymotrypsin catalyzed transesterification is described. Under present experimental conditions, and in a selective ion mode operation, ethyl furoate production can be detected with a sensitivity of 0. 1 mu M and a response time of approximately 13 s at 25 degree C.