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Pyranine phosphate as a new fluorigenic substrate for acidic and alkaline phosphatases

DOI: 10.1248/cpb.40.786

Source and publish data:

Chemical and Pharmaceutical Bulletin p. 786 - 788 (1992)

Update date:2022-08-17

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Authors:

SatoSato

ChibaChiba

HoshiHoshi

KanaokaKanaoka

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Article abstract of DOI:10.1248/cpb.40.786

8-Hydroxypyrene-1,3,6-trisulfonic acid (HPTS, pyranine) is highly fluorescent not only in the alkaline range but also at an acidic pH, and in a water-soluble fluorescent compound. By phosphorylation of a pyranine, its fluorescence intensity was quenched markedly. A pyranine phosphate was shown to be a potential fluorogenic substrate for the assay of acid and alkaline phosphatases, and also for phosphatase activities in human serum.

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Full text of DOI:10.1248/cpb.40.786

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