Chemical and Pharmaceutical Bulletin p. 786 - 788 (1992)
Update date:2022-08-17
Topics:
Sato
Chiba
Hoshi
Kanaoka
8-Hydroxypyrene-1,3,6-trisulfonic acid (HPTS, pyranine) is highly fluorescent not only in the alkaline range but also at an acidic pH, and in a water-soluble fluorescent compound. By phosphorylation of a pyranine, its fluorescence intensity was quenched markedly. A pyranine phosphate was shown to be a potential fluorogenic substrate for the assay of acid and alkaline phosphatases, and also for phosphatase activities in human serum.
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