Ester synthesis by NAD(+)-dependent dehydrogenation of hemiacetal: production of methyl formate by cells of methylotrophic yeasts.

Article abstract of DOI:10.1271/bbb.61.1391

A water-soluble ester, methyl formate, was detected as a metabolite in the culture medium of methylotrophic yeasts. Methyl formate synthase, which catalyses NAD(+)-dependent dehydrogenation of the hemiacetal adduct of methanol and formaldehyde, catalyses the ester synthesis. The enzyme activity was induced on a methanol medium and was increased further by the addition of formaldehyde. In the reaction system using intact cells of Pichia methanolica AKU 4262, 135 mM (8.1 g/liter) methyl formate was produced from 2 M methanol. This is a new biological process for ester synthesis that couples spontaneous formation of hemiacetal and alcohol dehydrogenase.

Full text of DOI:10.1271/bbb.61.1391

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Ester Synthesis by NAD+-dependent Dehydrogenation
of Hemiacetal: Production of Methyl Formate by
Cells of Methylotrophic Yeasts
Agung Primanto Murdanotoa, Yasuyoshi Sakaia, Langkah Sembiringa, Yoshiki Tania & Nobuo
Katoa
a Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University,
Kitashirakawa Sakyo-ku, Kyoto 606–01, Japan
Published online: 12 Jun 2014.
To cite this article: Agung Primanto Murdanoto, Yasuyoshi Sakai, Langkah Sembiring, Yoshiki Tani & Nobuo Kato
(1997) Ester Synthesis by NAD+-dependent Dehydrogenation of Hemiacetal: Production of Methyl Formate by Cells of
Methylotrophic Yeasts, Bioscience, Biotechnology, and Biochemistry, 61:8, 1391-1393, DOI: 10.1271/bbb.61.1391
To link to this article: http://dx.doi.org/10.1271/bbb.61.1391
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