10.1002/cctc.201901870
ChemCatChem
FULL PAPER
Into a 50 mL-erlenmeyer with a skirted stopper was poured 10 mL of a
medium containing 50 mM MOPS buffer pH 8.5, 10 mM (1 mL from a
solution of 100 mM) butyric acid (1), 100 mM (2 mL from a solution of 500
mM) methylamine, 0.5 mM (50 µL from a solution of 100 mM) ATP, 2 mM
(0.2 mL from a solution of 100 mM) PolyP25, 10 mM (1 mL from a solution
of 100 mM ) MnCl2, 10 mM (1 mL from a solution of 100 mM ) MgCl2, 1
mg (116 µL from a solution of 8.6 mg/mL, 1.63 µM) GtheCoAlig, 0.2 mg
(408 µL from a solution of 0.49 mg/mL, 0.66 µM) DgeoPPK2-III and 0.02
mg PhPPase (168 µL from a solution of 0.12 mg/mL, 0.1 µM). The
reaction mixture was stirred at 90 rpm in an Infors® incubation shaker at
60°C for 24 h and then quenched with 10 mL of a saturated solution of
ammonium chloride. The aqueous layer was extracted with ethyl acetate
(4 x 5 mL) and DCM (4 x 5 mL). The combined organic layers were
washed with saturated sodium carbonate (10 mL), dried over magnesium
sulfate, filtered and concentrated under reduced pressure to dryness to
get a crude yellow oil (7.8 mg, crude yield = 77%). The TN was calculated
as the moles of product divided by the moles of catalyst. NMR analyses
were identical to those of the synthetic standard (vide supra, “Synthesis of
N-methylbutyrylamide (4)”).
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Keywords: amides • ATP regeneration • biocatalysis • cascade
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