10
K. KOÇDEMIR ET AL.
C¸akar MM, Ruupunen J, Mangas-Sanchez J, Birmingham WR,
Yildirim D, Turunen O, Turner NJ, Valjakka J, Binay B.
Acknowledgements
The authors gratefully acknowledge the help and support of
ETDAM (Enzyme Testing and Consultation Center, Gebze
Technical University, Turkey).
2020.
Engineered
formate
dehydrogenase
from
Chaetomium thermophilum, a promising enzymatic solu-
tion for biotechnical CO2 fixation. Biotechnol Lett. 42(11):
2251–2262.
Choe H, Joo JC, Cho DH, Kim MH, Lee SH, Jung KD, Kim YH.
2014. Efficient CO2-reducing activity of NAD-dependent
formate dehydrogenase from Thiobacillus sp. KNK65MA
for formate production from CO 2 gas. PLoS One. 9(7):
e103111.
Disclosure statement
We have no conflict of interest to declare.
Authors’ contributions
Fernandez-Lafuente R. 2009. Stabilization of multimeric
enzymes: strategies to prevent subunit dissociation.
Enzyme Microb Technol. 45(6–7):405–418.
Florides GA, Christodoulides P. 2009. Global warming and
carbon dioxide through sciences. Environ Int. 35(2):
390–401.
Galkin AG, Kutsenko AS, Bajulina NP, Esipova NG, Lamzin VS,
Mesentsev AV, Shelukho DV, Tikhonova TV, Tishkov VI,
Ustinnikova TB, et al. 2002. Site-directed mutagenesis of
the essential arginine of the formate dehydrogenase
active centre. Biochim Biophys Acta. 1594(1):136–149.
BB has conceived and designed the study. BB, BY, MMC¸ and
YAW have predicted the mutations. KK, FS¸, YAW, BY, MMC¸,
MB, IS, and MCB have carried out mutagenesis and charac-
terization studies. MMC¸ has carried out protein modelling.
BB oversaw the project. The manuscript was written by KK,
FS¸, YAW, BY, MMC¸, ESB and BB.
Funding
This work was partially supported by a grant from the
TUBITAK [Grant number: 119Z155].
€ €
Glueck SM, Gumus S, Fabian WMF, Faber K. 2010.
Biocatalytic carboxylation. Chem Soc Rev. 39(1):313–328.
Hatrongjit R, Packdibamrung K. 2010. A novel NADPþ-
dependent formate dehydrogenase from Burkholderia sta-
bilis 15516: screening, purification and characterization.
Enzyme Microb Technol. 46(7):557–561.
References
Hawkins AS, McTernan PM, Lian H, Kelly RM, Adams MWW.
2013. Biological conversion of carbon dioxide and hydro-
gen into liquid fuels and industrial chemicals. Curr Opin
Biotechnol. 2013;24:376–384.
Kargov IS, Kleimenov SY, Savin SS, Tishkov VI, Alekseeva AA.
2015. Improvement of the soy formate dehydrogenase
properties by rational design. Protein Eng Des Sel. 28(6):
171–178.
Alekseeva AA, Serenko AA, Kargov IS, Savin SS, Kleymenov
SY, Tishkov VI. 2012. Engineering catalytic properties and
thermal stability of plant formate dehydrogenase by sin-
gle-point mutations. Protein Eng Des Sel. 25(11):781–788.
Alissandratos A, Kim HK, Easton CJ. 2013. Formate produc-
tion through biocatalysis. Bioengineered. 4(5):348–350.
Alissandratos A, Kim HK, Easton CJ. 2014. Formate produc-
tion through carbon dioxide hydrogenation with recom-
binant whole cell biocatalysts. Bioresour Technol. 164:
7–11.
Arnold K, Bordoli L, Kopp J, Schwede T. 2006. The SWISS-
MODEL workspace: a web-based environment for protein
structure homology modelling. Bioinformatics. 22(2):
195–201.
Aslan AS, Valjakka J, Ruupunen J, Yildirim D, Turner NJ,
Turunen O, Binay B. 2017. Chaetomium thermophilum for-
mate dehydrogenase has high activity in the reduction of
hydrogen carbonate (HCO3-) to formate. Protein Eng Des
Sel. 30(1):47–55.
Avilova TV, Egorova OA, Ioanesyan LS, Egorov AM. 1985.
Biosynthesis, isolation and properties of NAD-dependent
formate dehydrogenase from the yeast Candida methyl-
ica. Eur J Biochem. 152(3):657–662.
Bassegoda A, Madden C, Wakerley DW, Reisner E, Hirst J.
2014. Reversible interconversion of CO2 and formate by a
molybdenum-containing formate dehydrogenase. J Am
Chem Soc. 136(44):15473–15476.
Bolivar JM, Wilson L, Ferrarotti SA, Fernandez-Lafuente R,
Guisan JM, Mateo C. 2007. Evaluation of different immo-
bilization strategies to prepare an industrial biocatalyst of
formate dehydrogenase from Candida boidinii. Enzyme
Microb Technol. 40(4):540–546.
€
Karimaki J, Parkkinen T, Santa H, Pastinen O, Leisola M,
Rouvinen J, Turunen O. 2004. Engineering the substrate
specificity of xylose isomerase. Protein Eng Des Sel.
17(12):861–869.
Kim S, Kim MK, Lee SH, Yoon S, Jung KD. 2014. Conversion
of CO2 to formate in an electroenzymatic cell using
Candida boidinii formate dehydrogenase. J Mol Catal B:
Enzym. 102:9–15.
Labrou NE, Rigden DJ. 2001. Active-site characterization of
Candida boidinii formate dehydrogenase. Biochem J.
354(2):455–463.
Lamzin VS, Dauter Z, Popov VO, Harutyunyan EH, Wilson KS.
1994. High resolution structures of holo and apo formate
dehydrogenase. J Mol Biol. 236(3):759–785.
Lenz M, Fademrecht S, Sharma M, Pleiss J, Grogan G, Nestl
BM. 2018. New imine-reducing enzymes from b-hydroxya-
cid dehydrogenases by single amino acid substitutions.
Protein Eng Des Sel. 31(4):109–120.
Leopoldini M, Chiodo SG, Toscano M, Russo N. 2008.
Reaction mechanism of molybdoenzyme formate
dehydrogenase. Chemistry. 14(28):8674–8681.
Lu Y, Yi Jiang Z, Wei Xu S, Wu H. 2006. Efficient conversion
of CO2 to formic acid by formate dehydrogenase immobi-
lized in a novel alginate-silica hybrid gel. Catal Today.
115(1-4):263–268.