Esterase-Catalyzed Siderophore Hydrolysis Activates an Enterobactin-Ciprofloxacin Conjugate and Confers Targeted Antibacterial Activity

Article abstract of DOI:10.1021/jacs.8b01042

Enteric Gram-negative bacteria, including Escherichia coli, biosynthesize and deploy the triscatecholate siderophore enterobactin (Ent) in the vertebrate host to acquire iron, an essential nutrient. We report that Ent-Cipro, a synthetic siderophore-antibi

Full text of DOI:10.1021/jacs.8b01042

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Article
Esterase-Catalyzed Siderophore Hydrolysis Activates an Enterobactin–
Ciprofloxacin Conjugate and Confers Targeted Antibacterial Activity
Wilma Neumann, Martina Sassone-Corsi, Manuela Raffatellu, and Elizabeth M. Nolan
J. Am. Chem. Soc., Just Accepted Manuscript • DOI: 10.1021/jacs.8b01042 • Publication Date (Web): 26 Mar 2018
Downloaded from http://pubs.acs.org on March 26, 2018
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Esterase-Catalyzed Siderophore Hydrolysis Activates an Enterobactin–Ciprofloxacin
Conjugate and Confers Targeted Antibacterial Activity
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Wilma Neumann, Martina Sassone-Corsi, Manuela Raffatellu, and Elizabeth M. Nolan
1Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, United
States
2Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA
92697, United States
#Present address: Department of Pediatrics, University of California, San Diego, La Jolla, CA
92093, United States
*Corresponding author: lnolan@mit.edu
Phone: 617-452-2495
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Abstract
Enteric Gram-negative bacteria, including Escherichia coli, biosynthesize and deploy the
triscatecholate siderophore enterobactin (Ent) in the vertebrate host to acquire iron, an essential
nutrient. We report that Ent–Cipro, a synthetic siderophore–antibiotic conjugate based on the
native Ent platform that harbors an alkyl linker at one of the catechols with a ciprofloxacin cargo
attached, affords targeted antibacterial activity against E. coli strains that express the pathogen-
associated iroA gene cluster. Attachment of the siderophore to ciprofloxacin, a DNA gyrase
inhibitor and broad-spectrum antibiotic that is used to treat infections caused by E. coli, generates
an inactive prodrug and guides the antibiotic into the cytoplasm of bacteria that express the Ent
uptake machinery (FepABCDG). Intracellular hydrolysis of the siderophore restores the activity
of the antibiotic. Remarkably, Fes, the cytoplasmic Ent hydrolase expressed by all E. coli, does
not contribute to Ent–Cipro activation. Instead, this processing step requires IroD, a cytoplasmic
hydrolase that is only expressed by E. coli that harbor the iroA gene cluster and are predominantly
pathogenic. In the uropathogenic E. coli UTI89 and CFT073, Ent–Cipro provides antibacterial
activity comparable to unmodified ciprofloxacin. This work highlights the potential of leveraging
and targeting pathogen-associated microbial enzymes in narrow-spectrum antibacterial
approaches. Moreover, because E. coli include harmless gut commensals as well as resident
microbes that can contribute to disease, Ent–Cipro may provide a valuable chemical tool for strain-
selective modulation of the microbiota.
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Introduction
Bacterial infections, especially those caused by Gram-negative pathogens that have
developed resistance against antibiotics in clinical use, pose a tremendous threat to global health
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and motivate investigations of new antibacterial strategies. Moreover, an increasing appreciation
for the important role of the human microbiome in health and disease stimulates consideration of
how this complex community can be preserved during antibiotic therapy, as well as the
identification of strategies that can be used to modulate its composition to resolve pathological
conditions such as inflammatory bowel disease.2,3 The design and utilization of narrow-spectrum
antibiotics that target specific pathogens is one strategy to limit the spread of antibiotic resistance,4-
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and also provides chemical tools for fundamental studies that involve manipulating the
microbiota. The activity spectrum of established broad-spectrum antibiotics, such as b-lactams or
fluoroquinolones, can be narrowed by attaching a targeting moiety. For targeting Gram-negative
bacteria such as pathogenic Escherichia coli, outer membrane receptors provide opportunities for
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selective recognition and intracellular delivery. These b-barrel proteins are involved in the uptake
of various molecules that include essential nutrients.9
Transition metal ions are essential nutrients that bacterial pathogens must acquire from the
host environment. To starve invading microbial pathogens, the human host reduces metal
availability in a process termed 'nutritional immunity.'10 Bacteria utilize a variety of metal
acquisition systems to successfully colonize the host,11 and these machineries provide
opportunities for new antibiotic strategies. One strategy to acquire Fe(III) involves the secretion
of siderophores, which are low-molecular-weight iron chelators.12-14ꢀ Pathogenic strains are often
characterized by the utilization of multiple siderophores, which contribute to virulence and enable
them to outcompete other microbes colonizing the same niche.15,16
We have employed the native siderophore enterobactin (Ent, Figure 1A) for the targeted
delivery of small-molecule cargo, including antibiotics, to Gram-negative bacteria that utilize this
siderophore.17-19 Ent is a triscatecholate siderophore produced by various Gram-negative species
including E. coli, Salmonella enterica, and Klebsiella pneumoniae. The extraordinary iron-binding
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affinity of the triscatecholate (K ~10 M ) enables bacteria to extract iron from host proteins.
a
Ferric Ent is recognized by the outer membrane (OM) receptor FepA and transported into the
periplasm with energy provided by a TonB-ExbB-ExbD complex (Figure 1B). FepB, a periplasmic
binding protein, mediates translocation to the inner membrane (IM), and an ATP-binding cassette
(ABC) transporter, FepCDG, transports the ferric siderophore across the IM. Cytoplasmic release
of the bound iron requires hydrolysis of the trilactone by the esterase Fes, and is likely
accompanied by reduction of the metal ion.22-24 Several Ent producers also biosynthesize and
utilize salmochelins, C-glucosylated analogs of Ent (Figure 1A), to acquire iron and evade the Ent-
scavenging host-defense protein lipocalin-2 (also termed “siderocalin”).25-27 The C-
glycosyltransferase required for glucosylation of Ent is encoded by the pathogen-associated iroA
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gene cluster (iroBCDEN), along with additional transport proteins and hydrolase enzymes. In
particular, the OM transporter IroN enables uptake of the ferric salmochelins,29,30 and the esterase
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IroD hydrolyzes ferric salmochelins for iron release (Figure 1B). An additional periplasmic
hydrolase, IroE, is predicted to partially hydrolyze the salmochelin trilactone during export of the
siderophores.22,26,31
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Figure 1. Siderophores and siderophore uptake machinery relevant to this work. (A) Structures of
enterobactin (Ent) and salmochelin S4. (B) Cartoon depiction of the Ent and salmochelin transport
and processing machinery in E. coli. IroD can also catalyze the hydrolysis of ferric Ent. The
periplasmic esterase IroE is not shown. Reduction of Fe(III) to Fe(II) that occurs during release of
the metal ion is not catalyzed by the hydrolases. DHBS, 2,3-dihydroxybenzoyl serine; Glc,
glucose.
Some bacteria biosynthesize and deploy siderophores tethered to antibiotic moieties to
target and kill competitors that express the requisite siderophore receptor.8,32 These
“sideromycins” have inspired the design and chemical synthesis of many siderophore–drug
conjugates to target bacterial siderophore uptake machinery for antibiotic delivery.33-37ꢀIndeed,
conjugation of antibiotics with periplasmic targets, such as b-lactams, to siderophores can afford
significantly increased antibacterial activity against Gram-negative pathogens.18,38-46 However, to
the best of our knowledge, attempts to employ this strategy for delivering antibiotics with
cytoplasmic targets to Gram-negative strains have not yet succeeded. Conjugation of antibiotics
with cytoplasmic targets, such as fluoroquinolones, to siderophores usually results in significantly
attenuated activity of the drugs.47-60 Along these lines, we found that two Ent conjugates carrying
the fluoroquinolone ciprofloxacin, Ent–PEG
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–Cipro 1 and Ent–Cipro 2 (Chart 1), were inactive
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against E. coli K-12 as well as Pseudomonas aeruginosa PAO1, a Gram-negative opportunistic
pathogen that cannot biosynthesize Ent but expresses a receptor for ferric Ent.17
Ciprofloxacin is widely used in the clinic to treat a number of Gram-negative bacterial
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infections, including urinary tract infections (UTIs). It inhibits the DNA gyrase, a topoisomerase
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that plays an important role during DNA replication and transcription. Despite our prior report
and other failed attempts to identify siderophore–ciprofloxacin conjugates that exhibit growth
inhibitory activity against Gram-negative bacteria, we continued to investigate whether
ciprofloxacin can be a useful tool for studying siderophore-mediated antibiotic delivery to the
bacterial cytoplasm. During these efforts, we revisited conjugates 1 and 2 and uncovered that alkyl-
linked Ent–Cipro 2 exhibits potent antibacterial activity against select E. coli strains, including
uropathogenic E. coli (UPEC).
Herein, we report this discovery and studies that decipher the uptake and fate of Ent–Cipro
2. This conjugate is delivered into the cytoplasm of E. coli where it acts as a prodrug that is
activated by intracellular hydrolysis of the siderophore. This hydrolysis is catalyzed by the
salmochelin esterase IroD that is only expressed in strains harboring the iroA gene cluster. These
investigations reveal a new approach to convert broad-spectrum antibiotics into more selective
therapeutics by exploiting siderophore processing machinery predominantly used by pathogenic
strains.
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Chart 1. Chemical structures of the ciprofloxacin conjugates and hydrolytic products. For 3, only
one possible regioisomer of the ester is shown.
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Results
An Enterobactin–Ciprofloxacin Conjugate is Active Against Uropathogenic E. coli
The antibacterial activity of Ent–ciprofloxacin conjugates 1 and 2 (Chart 1) was evaluated
against a panel of non-pathogenic and uropathogenic E. coli strains in a minimal M9 medium
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previously employed in studies of siderophore–b-lactam conjugates. Although the conjugates
only differ in the linker between the antibiotic and siderophore, they exert strikingly different
antibacterial activity under these conditions. Whereas the PEG -linked conjugate 1 exhibits no
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growth inhibitory activity against any of the four tested strains (E. coli K-12, B, UTI89 and
CFT073), the alkyl-linked Ent–Cipro 2 exerts high antibacterial activity against two UPEC strains,
UTI89 and CFT073, with minimum inhibitory concentration (MIC) values similar to that of
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ciprofloxacin (0.1–1 µM) (Figures 2, S1, S2; Table S5). In agreement with our prior work, the
growth of the non-pathogenic laboratory strains E. coli K-12 and B is not affected by Ent–Cipro 2.
Given this potent and strain-specific antibacterial activity, Ent–Cipro 2 provides an excellent
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chemical tool to study the determinants for successful siderophore-mediated delivery of antibiotics
into the cytoplasm of Gram-negative pathogens.
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Figure 2. Antibacterial activity of ferric Ent–Cipro 2 against non-pathogenic and pathogenic
E. coli strains. (A) Laboratory strain E. coli K-12. (B) Laboratory strain E. coli B. (C)
Uropathogenic E. coli UTI89. (D) Uropathogenic E. coli CFT073. All assays were performed in
modified M9 medium (t = 20 h, T = 30 °C; mean ± SDM, n = 3). Ent–Cipro was pre-loaded with
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.9 equiv Fe(III); data for assays performed with apo Ent–Cipro are presented in Figure S2.
Growth Conditions and Iron Loading Influence Antibacterial Activity of Ent–Cipro 2
The siderophore biosynthesis and transport machinery is controlled by the ferric uptake
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regulator (Fur) and expressed under low-Fe conditions. Thus, studies of siderophore–antibiotic
conjugates are often performed employing a growth medium, such as Mueller Hinton Broth
(
MHB), supplemented with the iron chelator 2,2'-dipyridyl (DP). Guided by our prior studies of
the antibacterial activity of Ent-b-lactam conjugates against E. coli,18,19 we performed the
antibacterial activity assays in MHB (50%) with and without 200 µM DP, but observed no growth
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inhibitory activity for Ent–Cipro 2 against E. coli under these conditions (Figure S12).
Antibacterial activity was only observed in the modified M9 medium (Figures 2, S2), a low-iron
growth medium (0.6 µM iron content; Table S4) that is not supplemented with an iron chelator
such as DP. Thus, all subsequent studies with conjugate 2 were performed employing this medium.
Next, we supplemented the M9 medium with iron (10 or 50 µM). As expected, this supplement
attenuates the antibacterial activity of conjugate 2 because the expression of siderophore uptake
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machinery is repressed when sufficient iron is available (Figure S3). In contrast, the addition of
iron has negligible effect on the antibacterial activity of ciprofloxacin. These results indicate that
both iron levels and medium composition influence the antibacterial activity of Ent–Cipro 2, and
that these variables should be examined when assaying the growth inhibitory activity of
siderophore–antibiotic conjugates.
In addition, siderophore–antibiotic conjugates can be prepared and employed as either apo
or Fe(III)-bound molecules. To determine whether iron pre-loading affects the antibacterial
activity of Ent–Cipro 2, we treated E. coli with the apo conjugate or the ferric complex, and
observed that the Fe(III)-bound conjugate exhibits higher antibacterial activity than the apo
conjugate against the UPEC strains (Figure S2). Because the OM siderophore receptors recognize
and transport the ferric siderophores, iron pre-loading of the conjugate may facilitate rapid binding
at the receptor and thus uptake. In the case of the non-pathogenic strains K-12 and B, incubation
with apo conjugate 2 causes growth inhibition at high concentrations (i.e., 10 µM), indicating that
the siderophore causes an iron-withholding effect when present in excess to the iron content of the
growth medium. Moreover, this starvation effect suggests that the uptake of Ent–Cipro 2 into these
strains or the release of iron from the siderophore after uptake is impeded.
Ent–Cipro Enters E. coli Through the Ent Uptake Machinery
A major difference among the strains employed in this study is that the UPEC strains
UTI89 and CFT073 harbor the iroA gene cluster, whereas the laboratory strains K-12 and B do
not.64-66 Thus, both UTI89 and CFT073 produce and transport Ent as well as salmochelins. The
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observation that Ent–Cipro 2 is only active against the UPEC strains may suggest that it requires
the salmochelin transporter IroN and that uptake into the non-pathogenic strains that only express
FepA is impaired. To probe the cellular uptake pathway of Ent–Cipro 2, we employed the UPEC
strain CFT073 and generated six transporter mutants (Table S2). The fepA, iroN, and ihA mutants
and the fepA iroN double mutant were employed to probe recognition and transport across the OM.
The fepC mutant (ATPase) and the fepD fepG double mutant (IM translocase) were used to
investigate transport across the IM. Antibacterial activity assays with the OM transporter mutants
reveal that deletion of iroN does not affect the activity of the conjugate (Figures 3, S5). Moreover,
single deletion of fepA or ihA, an additional catecholate receptor expressed in E. coli CFT073,66,67
does not attenuate the activity of 2. Only double deletion of fepA and iroN abolishes the activity
of Ent–Cipro 2, indicating that the conjugate can enter E. coli CFT073 through both FepA and
IroN. This observation is in agreement with prior studies of Ent–b-lactam conjugates, which
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revealed that both FepA and IroN provide transport into the periplasm. In contrast, any deletion
of components of the IM transporter FepCDG abolishes the antibacterial activity of Ent–Cipro 2
(Figures 3, S6). This observation indicates that the conjugate is transported into the cytoplasm by
FepCDG, and that cytoplasmic delivery is required for E. coli growth inhibition. Overall, these
studies demonstrate that Ent–Cipro 2 crosses two membranes and enters the E. coli cytoplasm
through the Ent uptake machinery FepABCDG. IroN provides a second conduit for the conjugate
to cross the OM of CFT073, but is not essential.
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Figure 3. Antibacterial activity of ferric Ent–Cipro 2 against siderophore transporter mutants of
E. coli CFT073. (A) Wild-type and OM transporter knock-out mutants. (B) Wild-type and IM
transporter knock-out mutants. All assays were performed in modified M9 medium (t = 20 h,
T = 30 °C; mean ± SDM, n = 3). Ent–Cipro was pre-loaded with 0.9 equiv Fe(III); data for assays
performed with apo Ent–Cipro are presented in Figures S5, S6.
The low MIC values obtained for Ent–Cipro 2 against UPEC suggest that the conjugate is
not significantly outcompeted by endogenous siderophores for binding at the siderophore
receptors, at least under the assay conditions. Nevertheless, the conjugate exhibits slightly
increased antibacterial activity against an entC mutant of E. coli CFT073, which is unable to
biosynthesize Ent (Figure S8). When the UPEC strains are co-treated with Ent–Cipro 2 and Ent
(
1:1 molar ratio), growth promotion is observed at high concentrations (Figure S4), suggesting that
the conjugate can be outcompeted by the native siderophore for binding at the receptor when high
concentrations of the endogenous siderophore are present.
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Ent–Cipro Requires Intracellular Hydrolysis to Exert Antibacterial Activity
The uptake studies with the OM receptor mutants indicate that Ent–Cipro 2 likely enters
the cytoplasm of all E. coli strains through FepABCDG. Thus, we questioned if the ferric
siderophore processing machinery could account for the different susceptibilities of the strains. All
E. coli express the cytoplasmic ferric Ent hydrolase Fes, and strains that harbor the iroA gene
cluster also express the salmochelin hydrolase IroD in this locale. We therefore prepared two
hydrolase-deficient strains of CFT073 (a fes mutant and an iroD mutant), and examined the
susceptibility of these mutants to Ent–Cipro 2. Whereas deletion of the Ent hydrolase Fes does not
significantly affect its activity, deletion of the salmochelin hydrolase IroD abolishes the
antibacterial activity of Ent–Cipro 2 (Figures 4, S7). Moreover, complementation of E. coli K-12
with iroD makes this strain susceptible to the conjugate (Figures 4, S9). Taken together, these
results demonstrate that IroD plays a crucial role for the antibacterial activity of Ent–Cipro 2, and
indicate that the conjugate acts as a prodrug that is activated in the cytoplasm by IroD-catalyzed
hydrolysis of the siderophore. Consistent with these observations, incubation of Ent–Cipro 2 with
purified IroD shows that the enzyme catalyzes the hydrolysis of the attached siderophore
(Figures 5, S15, S18). Hydrolysis of the siderophore trilactone of 2 proceeds similarly to that of
Ent and results in release of 2,3-dihydroxybenzoyl serine (DHBS), as well as formation of
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(DHBS) –Cipro 3 and ultimately the monocatecholate DHBS–Cipro 4 (Chart 1, Scheme S1).
2
Overall, it appears that Ent–Cipro 2 must be hydrolyzed in the E. coli cytoplasm to exert
antibacterial activity, that Fes cannot perform this function sufficiently, and that the requirement
for IroD confers selectivity to Ent–Cipro 2 such that it only exhibits antibacterial activity against
E. coli strains that harbor the iroA gene cluster. E. coli include harmless gut commensal organisms,
pathogens and pathobionts (i.e., organisms that are generally harmless but can cause disease in
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some circumstances), but because the iroA gene cluster is associated with pathogenicity, Ent–
Cipro 2 targets E. coli strains that are problematic for human health.
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Figure 4. Antibacterial activity of ferric Ent–Cipro 2 against siderophore hydrolase mutants of
E. coli CFT073 and E. coli K-12. (A) Wild-type and hydrolase knock-out mutants of E. coli
CFT073. (B) E. coli K-12(DE3), wild-type and complemented with iroD. All assays were
performed in modified M9 medium (t = 20 h, T = 30 °C; mean ± SDM, n = 3). Ent–Cipro was pre-
loaded with 0.9 equiv Fe(III); data for assays performed with apo Ent–Cipro are presented in
Figures S7, S9.
Figure 5. Hydrolysis of the ferric siderophores by IroD. (A) Hydrolysis of ferric Ent to linear Ent
(
lin.), (DHBS)
Cipro 4, and DHBS. (C) Hydrolysis of ferric Ent–PEG
DHBS–PEG –Cipro (mon.), and DHBS. Analytical HPLC traces (316 nm absorption) from
2
, and DHBS. (B) Hydrolysis of ferric Ent–Cipro 2 to (DHBS)
2
–Cipro 3, DHBS–
3
–Cipro 1 to (DHBS) –PEG
2
3
–Cipro (dim.),
3
enzymatic activity assays performed with 100 µM ferric siderophore (pre-loaded with 1 equiv
Fe(III)) and 0.15 µM IroD in 75 mM Tris-HCl, pH 8.0. Data from additional time points, assays
with apo siderophores, and control assays are presented in Figures S13–S18; molecular structures
of the compounds are presented in Chart 1 and Scheme S1.
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Siderophore Conjugation Attenuates DNA Gyrase Inhibitory Activity of Ciprofloxacin
When ciprofloxacin inhibits DNA gyrase, the fluoroquinolone moiety intercalates into the
DNA that is bound by the gyrase, and the carboxyl group of the antibiotic participates in a metal-
ion–water bridge that stabilizes the drug–enzyme–DNA complex.69-72 Whereas modification and
neutralization of the carboxyl moiety result in inactivation, some structural modifications at the
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piperazinyl moiety are tolerated without significant loss of antibacterial activity. However,
attachment of large residues at the piperazine impairs binding at the DNA–gyrase complex. The
inhibition of E. coli DNA gyrase by Ent–Cipro 2 was tested by monitoring the negative
supercoiling of plasmid DNA in the presence of the enzyme. Consistent with prior reports of
siderophore–ciprofloxacin conjugates,47,56,58 the DNA gyrase inhibitory activity of Ent–Cipro 2
(IC50: 70 µM) is ≈280-fold lower than that of unmodified ciprofloxacin (IC50: 0.25 µM) (Table S8,
Figure S21). The final hydrolytic product DHBS–Cipro 4 (IC50: 20 µM) exhibits somewhat
enhanced antibacterial activity relative to Ent–Cipro 2, but ≈80-fold lower activity than
unmodified ciprofloxacin. These results indicate that modification of the piperazinyl moiety of
ciprofloxacin with an alkyl linker appended to Ent or DHBS attenuates its ability to inhibit DNA
gyrase. Moreover, it is difficult to reconcile the IC50 values obtained from this assay and the MIC
values obtained for Ent–Cipro 2 against UPEC. Together, these data may indicate that hydrolysis
product 4 is not the active species that inhibits DNA gyrase, that the active species inhibits a target
other than DNA gyrase, or that multiple targets including DNA gyrase exist. Alternatively,
siderophore-mediated delivery could result in an increased accumulation of DHBS–Cipro 4 in the
cytoplasm compared to ciprofloxacin, which may compensate for its lower DNA gyrase inhibitory
activity.
The Intact Siderophore is Required for Antibacterial Activity
Given that IroD and thus hydrolysis of conjugate 2 is essential for its antibacterial activity,
we questioned whether its hydrolytic products would exert inhibitory activity against all strains
independent of IroD expression. We performed large-scale degradation assays of Ent–Cipro 2 to
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obtain sufficient quantities of (DHBS)
2
–Cipro 3 and DHBS–Cipro 4 for bacterial susceptibility
testing against the UPEC strains as well as E. coli K-12 and B. Neither (DHBS) –Cipro 3 nor
2
DHBS–Cipro 4 exhibits growth inhibitory activity against the four strains evaluated (Figures 6,
S10, S11). Moreover, pre-loading of (DHBS) –Cipro 3 with Fe(III) (0.9 equiv) to enable formation
] complex, or addition of Fe(III) (0.9 equiv) and DHBS (1.0 equiv) to form
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of an [Fe(3)(H
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[Fe(3)(DHBS)] , which structurally resembles ferric Ent–Cipro 2, does not result in growth
inhibition. Lastly, growth inhibition does not occur for the CFT073 entC mutant (Figure S8),
indicating that displacement by the endogenous siderophore at the Ent receptors does not
significantly affect the activity of the DHBS conjugates. In total, these observations suggest that
the truncated siderophore conjugate may not be well recognized or transported by the siderophore
uptake machinery, and demonstrate that the intact Ent scaffold is required for the antibacterial
activity of the ciprofloxacin conjugate.
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Figure 6. Antibacterial activity of the hydrolytic products of Ent–Cipro 2, (DHBS) –Cipro 3 and
DHBS–Cipro 4, against uropathogenic E. coli strains. (A) E. coli UTI89. (B) E. coli CFT073. All
assays were performed in modified M9 medium (t = 20 h, T = 30 °C; mean ± SDM, n = 3). Ent–
2
Cipro and (DHBS) –Cipro were pre-loaded with 0.9 equiv Fe(III); data for assays performed with
2
additional strains and apo conjugate are presented in Figures S10, S11.
Discussion
Herein, we show that conjugation of the broad-spectrum antibiotic ciprofloxacin to Ent
narrows the activity spectrum of the antibiotic and affords selective growth inhibition of E. coli
strains that harbor the iroA gene cluster, and in particular UPEC. We elucidate that Ent–Cipro 2 is
delivered into the cytoplasm of E. coli strains through the Ent transport machinery FepABCDG
(
Figure 7). Moreover, the attached siderophore abolishes the antibacterial activity of ciprofloxacin
and the conjugate needs to be hydrolyzed after uptake to activate the prodrug. This hydrolysis step
requires the salmochelin esterase IroD that is only expressed in strains harboring the iroA gene
cluster. Many UPEC isolates carry this gene cluster within pathogenicity islands, and its genes are
expressed in vivo during urinary tract infection (UTI).73,74 Thus, it is possible that Ent–Cipro 2 may
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be effective in vivo in an UTI model while minimally perturbing the commensal microbiota. This
conjugate, and more broadly IroD-catalyzed prodrug activation, may also have utility for targeting
other infectious Gram-negative strains that harbor the iroA gene cluster and cause diseases that
range from food-borne illness to pneumonia and sepsis, including other pathogenic E. coli, S.
enterica and K. pneumoniae.19,26 Moreover, some E. coli are associated with inflammatory disease
and dysbiosis in the gut and “precision editing” of the gut microbiome is a topic of current
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interest. We posit that Ent–Cipro 2 and other siderophore–antibiotic conjugates may provide
useful tools for manipulating the gut microbiome.
Figure 7. Proposed model for the antibacterial action of Ent–Cipro 2.
The current work can be compared and contrasted to our reported studies of siderophore-
mediated b-lactam delivery to the E. coli periplasm.18,19 b-Lactam antibiotics such as ampicillin
and amoxicillin inhibit cell wall biosynthesis by inhibiting penicillin-binding proteins in the
periplasm. In this prior work, we established that Ent–b-lactam conjugates are transported into
E. coli via the OM transporters FepA and IroN and exert antibacterial activity in the periplasm.
Moreover, we demonstrated that substitution of the Ent for salmochelin S4 (Figure 1) provided
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targeted delivery of b-lactams into E. coli that express IroN. This strategy of antibiotic targeting
relies on conjugate recognition and transport by OM siderophore receptors expressed by
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pathogenic E. coli. The current work also targets E. coli that harbor the iroA cluster, but the
selectivity of Ent–Cipro 2 is based on the encoded cytoplasmic siderophore processing machinery.
To the best of our knowledge, Ent–Cipro 2 is the first reported synthetic siderophore–
antibiotic conjugate carrying a cytoplasmic antibiotic that exhibits antibacterial activity against
Gram-negative pathogens comparable to that of the unmodified antibiotic. The requirement of
intracellular processing and release of the antibiotic is reminiscent of the naturally occurring
albomycins, where intracellular release of the tRNA synthetase inhibitor from the ferrichrome-like
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siderophore is essential for the antibacterial activity. Intracellular release of antibiotics from
synthetic conjugates has been attempted by introducing cleavable linkers; however, these prior
investigations uncovered limitations. Ester linkages were employed to enable release of the
attached antibiotic by intracellular esterase- or acid-catalyzed hydrolysis, but the hydrolytic lability
of these ester bonds resulted in premature cleavage of the conjugates before uptake into the
cells.48,49,51,53,59,77-79 Another approach utilizing a trimethyl-lock linker based on a reduction-
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triggered cleavage mechanism did not yield a conjugate with high antibacterial activity. Ent–
Cipro 2 benefits from utilizing a native siderophore in that the cleavage of the conjugate is
associated with the endogenous siderophore processing machinery, which may be advantageous
to linkers that rely on separate cleavage mechanisms. On the other hand, a linker that enables
release of the unmodified antibiotic may provide a conjugate with higher activity, as long as
negligible premature cleavage before entry into the cytoplasm occurs and recognition and transport
of the conjugate are not impeded by the linker.
The impact of structural modifications on the transmembrane transport of the conjugate as
well as the exact mechanism of action of Ent–Cipro 2 warrant further investigations. Notably, the
hydrolytic product DHBS–Cipro 4 exhibits an IC50 value for inhibition of the DNA gyrase (20 µM)
significantly higher than the MIC value of Ent–Cipro 2 for the growth inhibition of the UPEC
strains (0.1–1 µM). At this point, it is not apparent whether DHBS–Cipro 4 is the actual active
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species or if the molecule is further metabolized during or after hydrolysis of the siderophore.
Future investigations in a cellular context should inform whether cytoplasmic accumulation occurs
to a greater extent for DHBS-Cipro 4 than for ciprofloxacin, which could account for the similar
MIC values of these compounds despite the differing IC50 values for DNA gyrase inhibition.
Moreover, further studies designed to identify the cellular target(s) of Ent–Cipro 4 should inform
whether DNA gyrase is the actual target inhibited by the conjugate.
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Our studies emphasize that delivery of siderophore–antibiotic conjugates into the
cytoplasm of Gram-negative bacteria strongly depends on the molecular structure of the conjugate.
Although PEG linkers are commonly employed because they provide flexibility and spatial
separation between different functional moieties, our prior and current observations suggest that
this linker may impede translocation across the IM. Given that the structurally-related Ent–PEG
3
–
b-lactam conjugates exhibited high antibacterial activity,18,19 it is possible that the PEG
-linked
3
conjugate 1 is transported across the OM by FepA/IroN but may get trapped in the periplasm. If
conjugate 1 was transported into the cytoplasm, we would expect an antibacterial activity similar
to that of Ent–Cipro 2 based on the observation that hydrolysis by IroD proceeds comparably
(Figures 5, S14, S17) and that the hydrolytic product exhibits similar inhibitory activity against
DNA gyrase (Table S8, Figure S21). However, the length or hydrophilicity of the PEG linker
3
could affect further intracellular processing of the hydrolytic products or inhibition of an as-yet
unidentified target.
Despite the fact that intracellular hydrolysis of Ent–Cipro 2 is essential for antibacterial
activity, conjugation of ciprofloxacin to the hydrolytic fragments of Ent abolishes the activity of
the antibiotic, indicating that a truncated siderophore likely impedes delivery into the cytoplasm.
Currently, the extent to which (DHBS) –Cipro 3 and DHBS–Cipro 4 are taken up into the cells is
2
unknown. In addition to the Ent transport machinery, uptake into the periplasm could be mediated
by the DHBS receptors (Fiu, Cir), as previously shown for b-lactam–catechol conjugates.39
However, transport across both the OM and IM may be hampered or fast efflux through the
siderophore export machinery or multi-drug efflux pumps may occur, and could prevent
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cytoplasmic accumulation of the conjugates to an amount sufficient for growth inhibition. Overall,
we reason that intact native siderophores are preferable for antibiotic delivery to ensure strong
recognition by the siderophore transporters and to minimize outcompetition by endogenous
siderophores, which was observed previously for a b-lactam conjugate with a truncated
siderophore.80
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The development of antibiotic resistance in microbial pathogens is a serious concern for
existing therapies as well as any new approach to treat microbial infections. We believe that future
studies that follow resistance to Ent–Cipro 2 in E. coli CFT073 and UTI89 will be informative.
For siderophore–antibiotic conjugates, resistance can occur via OM transporter mutations or
downregulation of siderophore uptake systems.81,82 Ent–Cipro 2 can enter E. coli through FepA
and IroN, and both transporters are important virulence factors that become upregulated during
urinary tract infection.73,74,83 It is possible that this feature makes resistance from OM transporter
mutations less likely because both transporters would need to acquire loss-of-function mutations.
For pathogenic strains that rely on Ent or the salmochelins for iron acquisition in the vertebrate
host, we reason that the loss or mutation of OM receptors that would prevent siderophore-based
targeting is unlikely. These organisms require iron for replication, and loss or mutation of OM
transporters such that the ferric siderophores are no longer transported would attenuate growth in
vivo.
In closing, this contribution uncovers that the siderophore uptake and processing
machinery can be leveraged to convert a broad-spectrum antibiotic into a narrow-spectrum
antibiotic. More broadly, this work highlights the potential of targeting pathogen-associated
microbial enzymes in narrow-spectrum antibacterial approaches. We look forward to examining
whether IroD-catalyzed hydrolysis can activate Ent-based conjugates harboring other cytoplasmic
warheads. We also hope that this study inspires new strategies that harness virulence-associated
mechanisms for combating infectious disease, limiting the spread of antibiotic resistance and
preserving the vitally important microbiota.
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Supporting Information
Complete experimental methods, Tables S1-S8, Figures S1-S30. This information is available free
of charge via the Internet at http://pubs.acs.org.
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Author Information
Corresponding Author: *lnolan@mit.edu
ORCID ID:
Wilma Neumann: 0000-0002-1728-5140
Martina Sassone-Corsi: 0000-0002-4137-8371
Manuela Raffatellu: 0000-0001-6487-4215
Elizabeth M. Nolan: 0000-0002-6153-8803
Notes: E.M.N. holds a patent related to this work.
Acknowledgements
We thank the NIH (Grants 1R21AI126465 and 1R01AI114625) for financial support; Prof. L.
Cegelski for providing E. coli UTI89; Prof. K. T. Hughes for providing plasmid pJK611; Dr. Dean
Gia Nguyen for technical assistance. W. N. acknowledges the German National Academy of
Sciences Leopoldina for a postdoctoral fellowship (LPDS 2015-08). M. R. holds an Investigator
in the Pathogenesis of Infectious Disease Award from the Burroughs Wellcome Fund.
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