Macrolide biosynthesis: A single cytochrome P450, PicK, is responsible for the hydroxylations that generate methymycin, neomethymycin, and picromycin in Streptomyces venezuelae

Article abstract of DOI:10.1016/S0960-894X(98)00553-8

The final step in the biosynthesis of methymycin, neomethymycin, and picromycin is an hydroxylation, shown to be carded out by the cytochrome P- 450 monooxygenase, PicK. Direct comparison of the relative k(cat)/K(m) values for the two substrates, YC-17 and narbomycin, showed a threefold rate preference of pick for narbomycin.